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http://dx.doi.org/10.4014/mbl.1410.10008

Development of Diagnostic PCR System for Three Seedtransmitted Quarantine Viruses Associated with Cucurbitaceae  

Lee, Siwon (Environmental Infrastructure Research Department, National Institute of Environmental Research)
Min, Byung-Dae (Environmental Infrastructure Research Department, National Institute of Environmental Research)
Lee, Jin-Young (Incheon International Airport Regional Office, Animal and Plant Quarantine Agency)
Shin, Yong-Gil (Incheon International Airport Regional Office, Animal and Plant Quarantine Agency)
Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
Jheong, Weon Hwa (Environmental Infrastructure Research Department, National Institute of Environmental Research)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.1, 2015 , pp. 79-83 More about this Journal
Abstract
The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.
Keywords
Cucurbitaceae; Plant quarantine; SqMV; CGMMV; KGMMV;
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Times Cited By KSCI : 8  (Citation Analysis)
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1 Park SJ, Lee JH, Nam M, Park CY, Kim JS, Lee JH, et al. 2011. Virus disease incidences and transmission ecology of oriental melons in seongju area. Res. Plant Dis. 17: 342−350.   DOI   ScienceOn
2 Lee SK, Song WY, Kim HM. 2004. Detection of Cucumber green mottle mosaic virus in bottle gourd seeds by RT-PCR. Res. Plant Dis. 10: 53-57.   DOI
3 Nelson M, McClelland M. 1992. Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes. Methods Enzymol. 216: 279-303.   DOI
4 Pan YB, Burner DM, Legendre BL. 2000. An assessment of the phylogenetic relationship among sugarcane and related taxa based on the nucleotide sequence of 5S rRNA intergenic spacers. Genetica. 108: 285-295.   DOI
5 Priou S, Gutarra L, Aley P. 2006. An improved enrichment broth for the sensitive detection of Ralstonia solanacearum (biovars 1 and 2A) in soil using DAS-ELISA. Plant Pathol. J. 55: 36-45.   DOI   ScienceOn
6 Rural Development Administration. 2012. Present condition and henceforward prospect of seed industry in the world. J. Korea Soc. Seed Sci. Ind. 9: 32-38.
7 Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95-98.
8 Shin YG. 2009. An Advanced Quarantine System for the Inspection of Seed-borne Viruses in Korea. Ph. D. thesis. Kyungpook National University, Daegu, Gyeongsangbuk-do, Korea.
9 Yang DC, Lee JS, Kim DW, Lim YP, Min BH. 1998. Efficient diagnosis of Cucumber Green Mottle Mosaic Virus in watermelon using RT-PCR and cloning of coat protein gene. Korean J. Plant Tissue Culture 25: 519-524.
10 Cho JD, Kim JS, Lee SH, Chung BN. 2007. Triplex virion capture (VC)/RT-PCR for three seed transmissible Tobamoviruses of CGMMV, ZGMMV and KGMMV occurring on Cucurbitaceae. Res. Plant Dis. 13: 82-87.   DOI
11 Lee HI, Kim JH, Yea MJ. 2010. Immunocapture RT-PCR for detection of seed-borne viruses on Cucurbitaceae Crops. Res. Plant Dis. 16: 121-124.   DOI
12 Lee S. 2013. A Study of Molecular Biological Detection Methods for Seed-transmitted Viruses in Quarantine. Ph. D. thesis. Dankook University, Cheonan, Chungcheongnam-do, Korea.
13 Lee S, Cha M, Kim SM, Heo NY, Shin YG, Lee SH. 2014. Development of nucleotide primers for diagnostic RT-PCR and nested PCR detection of three seed-transmitted viruses (CRLV, SpLV and WClMV) in quarantine. J. Agric. Life Sci. 48: 75-83.
14 Lee S, Kang EH, Chu YM, Shin YG, Ahn TY. 2013a. Development of PCR diagnosis system for plant quarantine seedborne Wheat streak mosaic virus. Korean J. Microbiol. 49:112-117.   DOI   ScienceOn
15 Animal, Plant and Fisheries Quarantine and Inspection Agency. 2013. List of plant quarantine viruses in Korea in newly revised in 2013. Res. Plant Dis. 19: 67-75.
16 Caruso P, Bertolini E, Cambra M, López MM. 2003. A new and sensitive co-operational polymerase chain reaction for rapid detection of Ralstonia solanacearum in water. J. Microbiol. Methods 55: 257-272.   DOI   ScienceOn
17 Lee S, Kang EH, Heo NY, Kim SM, Kim YJ, Shin YG. 2013b. Detection of Carnation necrotic fleck vrus and Carnation ring-spot virus using RT-PCR. Res. Plant Dis. 19: 36-44.   DOI   ScienceOn
18 Lee S, Kang EH, Shin YG, Lee SH. 2013c. Development of RT-PCR and nested PCR for detection of four quarantine plant viruses belonging to Nepovirus. Res. Plant Dis. 19: 220-225.   DOI   ScienceOn
19 Lee S, Shin YG. 2014. Development and practical use of RT-PCR for seed-transmitted Prune dwarf virus in quarantine. Plant Pathol. J. 30: 178-182.   DOI