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A survey of Kudoa septempunctata in olive flounder (Paralichthys olivaceus) hatcheries in the southwestern coast of Korea between 2014 and 2015

2014-2015년 남서해안 종묘장에서 생산된 넙치(Paralichthys olivaceus) 치어의 Kudoa septempunctata 감염실태 조사

  • Kim, Wi-Sik (Control Center for Aquatic Animal Diseases, Chonnam National University) ;
  • Kong, Kyoung-Hui (Department of Aqualife Medicine, Chonnam National University) ;
  • Jung, Sung-Ju (Control Center for Aquatic Animal Diseases, Chonnam National University) ;
  • Jung, Myung-Hwa (Department of Aqualife Medicine, Chonnam National University) ;
  • Jeon, Chan-Hyeok (Department of Marine Bioscience, Gangneung-Wonju National University) ;
  • Kim, Jeong-Ho (Department of Marine Bioscience, Gangneung-Wonju National University) ;
  • Oh, Myung-Joo (Control Center for Aquatic Animal Diseases, Chonnam National University)
  • 김위식 (전남대학교 수산질병관리원) ;
  • 공경희 (전남대학교 수산생명의학과) ;
  • 정성주 (전남대학교 수산질병관리원) ;
  • 정명화 (전남대학교 수산생명의학과) ;
  • 전찬혁 (강릉원주대학교 해양자원육성학과) ;
  • 김정호 (강릉원주대학교 해양자원육성학과) ;
  • 오명주 (전남대학교 수산질병관리원)
  • Received : 2015.07.22
  • Accepted : 2015.08.19
  • Published : 2015.08.30

Abstract

A survey was conducted to investigate the infection of Kudoa septempunctata in 660 olive flounder (Paralichthys olivaceus) (132 pooling samples) cultured in 11 hatcheries in 6 different regions of Korea between 2014 and 2015. Polymerase chain reaction (PCR) results were negative for K. septempunctata for all samples. Based on the kudoa diagnostic manual, K. septempunctata was not detected in olive flounder hatcheries.

2014년 1월부터 2015년 5월까지 충청남도, 전라북도 및 전라남도에 위치한 6개지지역 (충청남도: 태안, 보령, 전라북도: 고창, 전라남도: 무안, 영광, 완도) 총 11개소의 넙치 종묘장으로부터 채집한 넙치 치어 총 660마리 (132 pooling 시료)를 대상으로 Kudoa septempunctata의 감염현황을 조사하였다. 쿠도아 검사를 실시한 결과, 모든 넙치 종묘에 서 PCR 음성 결과가 확인되었다. 이상의 결과로 쿠도아 진단 매뉴얼 방법에 의해 조사된 종묘장의 넙치에서는 K. septempunctata가 검출되지 않는 것으로 나타났다.

Keywords

References

  1. Chun, S.K. Fish parasitology. Korea Fisheries Publishing Co., Korea, pp 123-178, 2006.
  2. Egusa, S., Wakabayashi, H. and Muroga, K. Infectious and parasitic diseases of fish and shellfish. Life Science Publishing Co., Korea, 2006.
  3. Grabner, D., Yokoyama, H., Shirakashi, S. and Kinami, R.: Diagnostic PCR assays to detect and differentiate Kudoa septempunctata, K. thyrsites and K. lateolabracis (Myxozoa, Multivalvulida) in muscle tissue of olive flounder (Paralichthys olivaceus). Aquaculture, 338: 36-40, 2012.
  4. Jeon, C.H., Wi, S., Song, J.Y., Choi, H.S. and Kim, J.H. Development of loop-mediated isothermal amplification method for detection of Kudoa septempunctata (Myxozoa: Multivalvulida) in olive flounder (Paralichthys olivaceus). Parasitol. Res., 113: 1759-1767, 2014. https://doi.org/10.1007/s00436-014-3821-0
  5. Kaur, H.: Myxozoan infestation in freshwater fishes in wetlands and aquaculture in punjab (India). Adv. Anim. Vet. Sci., 2: 488-502, 2014. https://doi.org/10.14737/journal.aavs/2014/2.9.488.502
  6. Kawai, T., Sekizuka, T., Yahata, Y., Kuroda, M., Kumeda, Y., Iijima, Y., Kamata, Y., Sugita-Konishi, Y. and Ohnishi, T.: Identification of Kudoa septempunctata as the causative agent of novel food poisoning outbreaks in Japan by consumption of Paralichthys olivaceus in raw fish. Clin. Infect. Dis., 54: 1046-1052, 2012. https://doi.org/10.1093/cid/cir1040
  7. Lom, J. and Dykova, I.: Myxosporea (Phylum Myxozoa). Fish diseases and disorders, Vol 1. Protozoan and metazoan infections. In: Woo PTK (ed). CAB International, USA, pp 97-148, 1995.
  8. Matsukane, Y., Sato, H., Tanaka, S., Kamata, Y. and Sugita-Konishi, Y.: Kudoa septempunctata n. sp. (Myxosporea: Multivalvulida) from an aquacultured olive flounder (Paralichthys olivaceus) imported from Korea. Parasitol. Res., 107: 865-872, 2010. https://doi.org/10.1007/s00436-010-1941-8
  9. Moran, J.D.W., Whitaker, D.J. and Kent, M.L.: A review of the myxosporean genus Kudoa Meglitsch, 1947, and its impact on the international aquaculture industry and commercial fisheries. Aquaculture, 172: 163-196, 1999. https://doi.org/10.1016/S0044-8486(98)00437-2
  10. NFRDI (National Fisheries Research and Development Institute). Kudoa diagnostic manual, 2014.
  11. Ohnishi, T, Kikuchi, Y, Furusawa, H, Kamata, Y. and Sugita- Konishi, Y.: Kudoa septempuncatata invasion increases the permeability of human intestinal epithelial monolayer. Foodborne Pathog. Dis., 10: 137-142, 2013. https://doi.org/10.1089/fpd.2012.1294
  12. Song, J.Y., Choi, J.H., Choi, H.S., Jung, S.H. and Park, M.A. Monitoring of Kudoa septempunctata in cultured olive flounder and wild fish in Jeju island during 2012. J. Fish Pathol., 26: 129-137, 2013. https://doi.org/10.7847/jfp.2013.26.3.129
  13. Song, J.Y., Kim, M.J., Choi, H.S. and Jung, S.H. Monitoring Kudoa septempunctata in cultured olive flounder Paralichthys olivaceus in different regions of Korea in 2013. Kor. J. Fish. Aquat. Sci., 47: 611-621, 2014.

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  2. Foodborne Illness Outbreaks in Gyeonggi Province, Korea, Following Seafood Consumption Potentially Caused by Kudoa septempunctata between 2015 and 2016 vol.9, pp.2, 2018, https://doi.org/10.24171/j.phrp.2018.9.2.05