한국어병학회지 (Journal of fish pathology)

  • 제28권2호
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  • Pages.71-78
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  • 2015
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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신속한 항생제 감수성 테스트 법의 개발을 위한 Water-Soluble Tetrazolium Salt의 적용

Application of water-soluble tetrazolium salt for development of rapid antimicrobial susceptibility testing methods

  • 황성돈 (국립수산과학원 수산생물방역과) ;
  • 조동희 (경상대학교 해양생명과학과) ;
  • 김광일 (국립수산과학원 수산생물방역과) ;
  • 조미영 (국립수산과학원 수산생물방역과) ;
  • 지보영 (국립수산과학원 수산생물방역과) ;
  • 박명애 (국립수산과학원 수산생물방역과) ;
  • 박찬일 (경상대학교 해양생명과학과)
  • Hwang, Seong Don (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Jo, Dong Hee (Department of Marine Biology and Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Kim, Gwang-Il (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Cho, Mi Young (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Jee, Bo Young (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Park, Myoung-Ae (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Park, Chan-Il (Department of Marine Biology and Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
  • 투고 : 2015.05.10
  • 심사 : 2015.08.17
  • 발행 : 2015.08.30
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초록

In this study, we conducted to the development of a rapid antimicrobial susceptibility test method using WST-1 which is known to water-soluble tetrazolium salt, in order to rapidly response against bacterial diseases in fish. Eight of antibiotics which are permissioned for marine organism from government were used to rapid antimicrobial susceptibility testing using the WST-1. As a result, a similar tendency was verified compare to conventional antibiotic susceptibility test results. Generally, the antibiotic susceptibility test method required about 3 days (72 hours) for determine the effective antibiotics, however, we have confirmed that the our method using WST-1 was required at least 36 hours in this study. Consequentially, our method will contribute to development of rapid antimicrobial susceptibility testing for bacterial diseases in fish.

키워드

참고문헌

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