Pediatric Infection and Vaccine

The Korean Society of Pediatric Infectious Diseases (대한소아감염학회)
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Distribution of Human Rotavirus Genotypes in a Tertiary Hospital, Seoul, Korea During 2009-2013

2009년부터 2013년까지 서울의 일개 대학병원에서 동정된 로타바이러스 유전형의 분포

  • Han, Tae Hee (Department of Diagnostic Laboratory Medicine, Sanggyepaik Hospital, Inje University College of Medicine) ;
  • Park, Sang-Hun (Seoul Health Environmental Center, Virus Team) ;
  • Chung, Ju-Young (Department of Pediatrics, Sanggyepaik Hospital, Inje University College of Medicine) ;
  • Hwang, Eung-Soo (Department of Microbiology and Immunology, Seoul National University School of Medicine)
  • 한태희 (인제의대 상계백병원 진단검사의학교실) ;
  • 박상훈 (서울시 보건환경연구원 바이러스팀) ;
  • 정주영 (인제의대 상계백병원 소아청소년과) ;
  • 황응수 (서울의대 미생물학교실)
  • Received : 2015.03.30
  • Accepted : 2015.06.15
  • Published : 2015.08.25
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Abstract

Purpose: Group A rotavirus (RV) is most common etiologic agent of acute gastroenteritis (AGE) in children worldwide. Recently, vaccination has been introduced in several countries to reduce the disease burden caused by RV infections, but continuous surveillance of RV strains is necessary to detect the emergence of potential variants induced by vaccine-immune pressure. This study aimed to investigate the changing pattern of RV genotypes in children with AGE, following the introduction of vaccination in Korea. Methods: Genotyping of RVs by RT-PCR on the basis of VP7 and VP4 gene segment sequence was carried out on 201 rotavirus-positive stool samples, from children hospitalized with AGE between August 2009 and June 2013. We have directly sequenced PCR products and analyzed the phylogenetic tree. Results: The most prevalent G genotype was G9 (33.3%), followed by G1 (22.4%), G3 (15.9%), G2 (6.0%), G4 (3.0%), G10 (1.5%), and mixed G-type (15.4%), with some nontypeable cases (2.5%). The detected P genotypes were P[4] (45.3%), P[8] (43.8%), mixed P-type (10.4 %), and P[2] (0.5%). The G9P[4] genotype was predominantly observed in hospitalized cases in Seoul in 2010/2011, however G1P[8] has been re-emerged as the predominant genotype in the following season (P =0.004). Conclusions: It seems that the periodic fluctuation in predominance of the G1, G3, and G9 strains occurred in Korea during 2009-2013, following the introduction of RV vaccination.

목적: 한국에 백신이 도입된 이후 장염으로 입원한 소아에서 확인된 로타바이러스 유전형의 분포를 알아보고자 하였다. 방법: 2009년 8월부터 2013년 6월 사이에 급성 위장관염으로 입원한 소아에게서 수집된 201개의 로타바이러스 양성 대변 검체를 대상으로 로타바이러스 유전형 분석이 시행되었다. 결과: G 유전형은 G9 (33.3%), G1 (22.4%), G3 (15.9%), G2 (6.0%), G4 (3.0%), G10 (1.5%)이었고 혼합형(15.4%) 및 분류불능(2.5%)이 관찰되었다. P 유전형은 P[4] (45.3%)과 P[8] (43.8%)가 주로 검출되었고 혼합형(10.4%)과 P[2] (0.5%)가 발견되었다. G9P[4] 유전형이 2010/2011년에 자주 검출되었으나 이후에 G1P[8] 유전형이 주로 검출되었다. 결론: 로타바이러스 백신 도입 이후 2009-2013년 동안 G1, G3 및 G9이 번갈아가며 주로 검출되는 것을 확인하였다.

Keywords

References

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