Browse > Article
http://dx.doi.org/10.14776/piv.2015.22.2.81

Distribution of Human Rotavirus Genotypes in a Tertiary Hospital, Seoul, Korea During 2009-2013  

Han, Tae Hee (Department of Diagnostic Laboratory Medicine, Sanggyepaik Hospital, Inje University College of Medicine)
Park, Sang-Hun (Seoul Health Environmental Center, Virus Team)
Chung, Ju-Young (Department of Pediatrics, Sanggyepaik Hospital, Inje University College of Medicine)
Hwang, Eung-Soo (Department of Microbiology and Immunology, Seoul National University School of Medicine)
Publication Information
Pediatric Infection and Vaccine / v.22, no.2, 2015 , pp. 81-90 More about this Journal
Abstract
Purpose: Group A rotavirus (RV) is most common etiologic agent of acute gastroenteritis (AGE) in children worldwide. Recently, vaccination has been introduced in several countries to reduce the disease burden caused by RV infections, but continuous surveillance of RV strains is necessary to detect the emergence of potential variants induced by vaccine-immune pressure. This study aimed to investigate the changing pattern of RV genotypes in children with AGE, following the introduction of vaccination in Korea. Methods: Genotyping of RVs by RT-PCR on the basis of VP7 and VP4 gene segment sequence was carried out on 201 rotavirus-positive stool samples, from children hospitalized with AGE between August 2009 and June 2013. We have directly sequenced PCR products and analyzed the phylogenetic tree. Results: The most prevalent G genotype was G9 (33.3%), followed by G1 (22.4%), G3 (15.9%), G2 (6.0%), G4 (3.0%), G10 (1.5%), and mixed G-type (15.4%), with some nontypeable cases (2.5%). The detected P genotypes were P[4] (45.3%), P[8] (43.8%), mixed P-type (10.4 %), and P[2] (0.5%). The G9P[4] genotype was predominantly observed in hospitalized cases in Seoul in 2010/2011, however G1P[8] has been re-emerged as the predominant genotype in the following season (P =0.004). Conclusions: It seems that the periodic fluctuation in predominance of the G1, G3, and G9 strains occurred in Korea during 2009-2013, following the introduction of RV vaccination.
Keywords
Rotavirus; Genotype; Gastroenteritis; Vaccine;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Matthijnssens J, Ciarlet M, McDonald SM, Attoui H, Banyai K, Brister K, et al. Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol 2011;156:1397-413.   DOI   ScienceOn
2 Matthijnssens J, Van Ranst M. Genotype constellation and evolution of group A rotaviruses infecting humans. Curr Opin Virol 2012;2:426-33.   DOI
3 Banyai K, Laszlo B, Duque J, Steele AD, Nelson EA, Gentsch JR, et al. Systemic review of regional and temporal trends in global rotavirus strain diversity in the pre rotavirus vaccine era: insights for understanding the impact of rotavirus vaccine programs. Vaccine 2012;30:A122-30.   DOI
4 Than VT, Jeong S, Kim W. A systemic review of genetic diversity of human rotavirus circulating in South Korea. Infect Genet Evol 2014;28:462-91.   DOI
5 Han TH, Kim CH, Chung JY, Park SH, Hwang ES. Genetic characterization of rotavirus in children in South Korea from 2007 to 2009. Arch Virol 2010;155:1663-73.   DOI
6 Carvalho-Costa FA, Araujo IT, de Assis RMS, Fialho AM, Martins CM, Boja MN, et al. Rotavirus genotype distribution after vaccine introduction, Rio de Janeiro, Brazil. Emerg Infect Dis 2009;15:95-7.   DOI
7 Gurgel RQ, Cuevas LE, Vieira SC, Barros VC, Fontes PB, Salustino EF, et al. Predominance of rotavirus P[4]G2 in a vaccinated population, Brazil. Emerg Infect Dis 2007;13:1571-3.   DOI
8 Kirkwood CD, Boniface K, Barnes GL, Bishop RF. Distribution of rotavirus genotypes after introduction of rotavirus vaccines, Rotarix$^{(R)}$ and RotaTeq$^{(R)}$, into the National Immunization Program of Australia. Pediatr Infect Dis J 2011;30:S48-53.   DOI
9 Choe YJ, Yang JJ, Park SK, Choi EH, Lee HJ. Comparative estimation of coverage between national immunization program vaccines and non-NIP vaccines in Korea. J Korean Med Sci 2013;28:1283-8.   DOI   ScienceOn
10 Lee SG, Jeon SY, Kim KY. 2012 Korea National Immunization Survey [internet]. Korean CDC [cited 2014 Aug 4]. Available from http://www.cdc.go.kr/CDC/info/CdcKrInfo0201.jsp?menuIds=HOME001-MNU1155-MNU1083-MNU1375-MNU0025&cid=20768.
11 Iturriza-Gomara M, Kang G, Gray J. Rotavirus genotyping:keeping up with an evolving population of human rotaviruses. J Clin Virol 2004;31:259-65.   DOI
12 Banerjee I, Ramani S, Primrose B, Iturriza-Gomara M, Gray JJ, Brown DW, et al. Modification of rotavirus multiplex RT-PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India. J Med Virol 2007;79:1413-21.   DOI
13 Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 1990;28:276-82.
14 Gentsch JR, Glass RI, Woods P, Gouvea V, Flores J, Das BK, et al. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 1992;30:1365-73.
15 Tamura K, Dudley J, Nei M, Kumar S. MEGA 4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007;24:1596-9.   DOI
16 Choi UY, Lee SY, Ma SH, Jang YT, Kim JY, Kim HM, et al. Epidemiological changes in rotavirus gastroenteritis in children under 5 years of age after the introduction of RV vaccines in Korea. Eur J Pediatr 2013;172:947-52.   DOI
17 da Silva MF, Gomez MM, Rose TL, Volotao Ede M, Carvalho-Costa FA, Bello G, et al. VP8*P[8] lineages of group A rotaviruses circulating over 20 years in Brazil: proposal of six different sub-lineages for P[8]-3 clade. Infect Genet Evol 2013;16:200-5.   DOI
18 Linhares AC, Stupka JA, Ciapponi A, Bardach AE, Glujovsky D, Aruj PK, et al. Burden and typing of rotavirus group A in Latin America and the Caribbean: systematic review and meta-analysis. Rev Med Virol 2011;21:89-109.   DOI
19 Huh JW, Kim WH, Yoon MH, Lim YH. Genotypic distribution of rotavirus strains causing severe gastroenteritis in Gyeonggi province, South Korea, from 2003 to 2005. Arch Virol 2009;154:167-70.   DOI
20 Martinez M, Amarilla AA, Galeano ME, Aquino VH, Farina N, Russomando G, et al. Predominance of rotavirus G2P[4] and emergence of G12P[9] strains in Asuncion, Paraguay, 2006-2007. Arch Virol 2010;155:525-33.   DOI
21 Jeong HS, Lee KB, Jeong AY, Jo MY, Jung SY, Ahn JH, et al. Genotypes of the circulating rotavirus strains in the seven prevaccine seasons from September 2000 to August 2007 in South Korea. Clin Microbiol Infect 2011;17:232-5.   DOI   ScienceOn
22 Kang JO, Kilgore P, Kim JS, Nyambat B, Kim J, Suh HS, et al. Molecular epidemiological profile of rotavirus in South Korea, July 2002 through June 2003; emergence of G4P[6] and G9P[8] strains. J Infect Dis 2005;192:S57-63.   DOI
23 Kim JS, Kang JO, Cho SC, Jang YT, Min SA, Park TH, et al. Epidemiological profile of rotavirus infection in the Republic of Korea results from prospective surveillance in the Jeongeub District, 1 July 2002 through 30 June 2004. J Infect Dis 2005;195:S49-56.
24 Shim JO, Thai Than V, Ryoo E, Lim I, Yoon Y, Kim K, et al. Distribution of rotavirus G and P genotypes approximately two years following the introduction of rotavirus vaccines in South Korea. J Med Virol 2013;85:1307-12.   DOI
25 Hull JJ, Teel EN, Kerin TK, Freeman MM, Esona MD, Gentsch JR, et al, National Rotavirus Surveillance System. United States rotavirus strain surveillance from 2005 to 2008: genotype prevalence before and after vaccine introduction. Pediatr Infect Dis J 2011;30:S42-7.   DOI
26 Esona MD, Banyai K, Foytich K, Freeman M, Mijatovic- Rustempasic S, Hull J, et al. Genomic characterization of human rotavirus G10 strains from the African Rotavirus Network: relationship to animal rotaviruses. Infect Genet Evol 2011;11:237-41.   DOI
27 Bok K, Matson DO, Gomez JA. Genetic variation of capsid protein VP7 in genotype g4 human rotavirus strains: simultaneous emergence and spread of different lineages in Argentina. J Clin Microbiol 2002;40:2016-22.   DOI
28 Bucardo F, Karlsson B, Nordgren J, Paniaqua M, Gonzalez A, Amador JJ, et al. Mutated G4P[8] rotavirus associated with a nationwide outbreak of gastroenteritis in Nicargua in 2005. J Clin Microbiol 2007;45:990-7.   DOI
29 Matthijnssens J, Helen E, Zeller M, Rahman M, Lemey P, Van Ranst M. Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Mol Biol Evol 2010;27:2431-6.   DOI
30 Chitambar SD, Ranshing SS, Pradhan GN, Kalrao VR, Dhongde RK, Bavdekar AR. Changing trends in circulating rotavirus strains in Pune, western India in 2009-2012: Emergence of a rare G9P[4] rotavirus strain. Vaccine 2014;32:A29-32.   DOI
31 Tatte VS, Chothe NS, Chitambar SD. Characterisation of rotavirus strains identified in adolescents and adults with acute gastroenteritis highlights circulation of non-typeable strains: 2008-2012. Vaccine 2014;32 Suppl 1:A68-74.   DOI
32 Afrad MH, Rahman MZ, Matthijnssens, Das SK, Faruque AS, Azim T, et al. High incidence of reassortant G9P[4] rotavirus strain in Bangladesh: Fully heterotypic from vaccine strains. J Clin Virol 2013;58:755-6.   DOI
33 Yen C, Figueroa JR, Uribe ES, Carmen-Hernandez LD, Tate JE, Parashar UD, et al. Monovalent rotavirus vaccine provides protection against an emerging fully heterotypic G9P[4] rotavirus strain in Mexico. J Infect Dis 2011;204:783-6.   DOI
34 Hemming M, Vesikari T. Genetic diversity of G1P[8] rotavirus VP7 and VP8 antigens in Finland over a 20-year period: no evidence for selection pressure by universal mass vaccination with $RotaTeq^{(R)}$ vaccine. Infect Genet Evol 2013;19:51-8.   DOI
35 Afrad MH, Hassan Z, Farjana S, Moni S, Barua S, Das SK, et al. Changing profile of rotavirus genotypes in Bangladesh, 2006-2012. BMC Infect Dis 2013;13:e320.   DOI