DOI QR코드

DOI QR Code

Effect of Microbial and Chemical Combo Additives on Nutritive Value and Fermentation Characteristic of Whole Crop Barley Silage

  • Kim, Dong Hyeon (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University) ;
  • Amanullah, Sardar M. (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University) ;
  • Lee, Hyuk Jun (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University) ;
  • Joo, Young Ho (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University) ;
  • Kim, Sam Churl (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University)
  • Received : 2015.02.08
  • Accepted : 2015.04.29
  • Published : 2015.09.01

Abstract

This study was conducted to assess the effects of microbial and chemical combo additives on nutritive values, fermentation indices and aerobic stability of whole crop barley silage. Barley forage (Youngyang) was harvested at about 30% dry matter (DM) by treatments, chopped to 5 cm length and treated with distilled water only (CON), Lactobacillus plantarum (INO), propionic acid (PRO) or an equal mixture of INO and PRO (MIX). Barley forages were ensiled in 4 replications for 0, 2, 7, and 100 days. On 100 days of ensiling, MIX silage had higher (p<0.05) in vitro DM digestibility than CON silage, but lower (p<0.05) acid detergent fiber concentration. The pH in all treated silages was lower (p<0.05) than CON silage. The MIX silage had higher (p<0.05) lactate concentration and lactate to acetate ratio than in CON, but lower (p<0.05) yeast count. Aerobic stability in CON, PRO, and MIX silages were higher (p<0.05) than in INO silage. It is concluded that microbial and chemical combo additives using L. plantarum and propionic acid could efficiently improve nutritive values of barley silage in terms of increased in vitro DM digestibility compared to other treatments. In addition, all treatments except CON reduced yeast count which is the initiate microorganism of aerobic spoilage.

Keywords

References

  1. Adesogan, A. T., L. E. Sollenberger, Y. C. Newman, and J. M. B. Vendramin. 2002. Factors affecting forage quality. In: Florida Forage Handbook (Ed. Y. C. Newman). University of Florida IFAS Exension, Gainesville, FL, USA. pp. 179-183.
  2. Adesogan, A. T., N. Krueger, M. B. Salawu, D. B. Dean, and C. R. Staples. 2004. The influence of treatment with dual purpose bacterial inoculants or soluble carbohydrates on the fermentation and aerobic stability of bermudagrass. J. Dairy Sci. 87:3407-3416. https://doi.org/10.3168/jds.S0022-0302(04)73476-1
  3. Amanullah, S. M., D. H. Kim, H. J. Lee, Y. H. Joo, S. B. Kim, and S. C. Kim. 2014. Effects of microbial additives on chemical composition and fermentation characteristics of barley silage. Asian Australas. J. Anim. Sci. 27:511-517. https://doi.org/10.5713/ajas.2013.13617
  4. AOAC. 1995. Official Methods of Aanalysis. 15th edn, Association of Official Analytical Chemists, Arlington, VA, USA.
  5. Baah, J., W. Addah, E. K. Okine, and T. A. McAllister. 2011. Effects of homolactic bacterial inoculant alone or combined with an anionic surfactant on fermentation, aerobic stability and in situ ruminal degradability of barley silage. Asian Australas. J. Anim. Sci. 24:369-378. https://doi.org/10.5713/ajas.2011.10320
  6. Bolsen, K. K., D. R. Bonilla, G. L. Huck, M. A. Young, and R. A. Hart-Thakur. 1996. Effect of a propionic acid bacterial inoculant on fermentation and aerobic stability of whole-plant corn silage. Kansas Agric. Exp. Stn. Res. Rep. 756:77-88.
  7. Chaney, A. L. and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130-132.
  8. Cleale R. M. IV, J. L. Firkins, F. Van Der Beek, J. H. Clark, E. H. Jaster, G. C. McCoy, and T. H. Klusmeyer. 1990. Effect of inoculation of whole plant corn forage with Pediococcus acidilactici and Lactobacillus xylosus on preservation of silage and heifer growth. J. Dairy Sci. 73:711-718. https://doi.org/10.3168/jds.S0022-0302(90)78724-3
  9. Courtin, M. G. and S. F. Spoelstra. 1990. A simulation model of the microbiological and chemical changes accompanying the initial stage of aerobic deterioration of silage. Grass Forage Sci. 45:153-165. https://doi.org/10.1111/j.1365-2494.1990.tb02196.x
  10. Driehuis. F., S. J. W. H. Oude Elferink, and P. G. Van Wikselaar. 2001. Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria. Grass Forage Sci. 56:330-343. https://doi.org/10.1046/j.1365-2494.2001.00282.x
  11. Filya I. and E. Sucu. 2007. The effect of bacterial inoculants and a chemical preservative on the fermentation and aerobic stability of whole-crop cereal silages. Asian Australas. J. Anim. Sci. 20:378-384. https://doi.org/10.5713/ajas.2007.378
  12. Hargreaves, A., J. Hill, and J. D. Leaver. 2009. Effect of stage of growth on the chemical composition, nutritive value and ensilability of whole-crop barley. Anim. Feed Sci. Technol. 152:50-61. https://doi.org/10.1016/j.anifeedsci.2009.03.007
  13. Henderson, N. 1993. Silage additives. Anim. Feed Sci. Technol. 45:35-56. https://doi.org/10.1016/0377-8401(93)90070-Z
  14. Higginbotham G. E., S. C. Mueller, K. K. Bolsen, and E. J. DePeters. 1998. Effects of inoculants containing propionic acid bacteria on fermentation and aerobic stability of corn silage. J. Dairy Sci. 81:2185-2192. https://doi.org/10.3168/jds.S0022-0302(98)75797-2
  15. Hristov, A. N. and T. A. McAllister. 2002. Effect of inoculants on whole-crop barley silage fermentation and dry matter disappearance in situ. J. Anim. Sci. 80:510-516.
  16. Kung, L., Jr. and N. K. Ranjit. 2001. The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. J. Dairy Sci. 84:1149-1155. https://doi.org/10.3168/jds.S0022-0302(01)74575-4
  17. Kung, L., Jr., J. R. Robinson, N. K. Ranjit, J. H. Chen, C. M. Golt, and J. D. Pesek. 2000. Microbial populations, fermentation end-products, and aerobic stability of corn silage treated with ammonia or a propionic acid-based preservative. J. Dairy Sci. 83:1479-1486. https://doi.org/10.3168/jds.S0022-0302(00)75020-X
  18. Kung, L., Jr., A. C. Sheperd, A. M. Smagala, K. M. Endres, C. A. Bessett, N. K. Ranjit, and J. L. Glancey. 1998. The effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration. J. Dairy Sci. 81:1322-1330. https://doi.org/10.3168/jds.S0022-0302(98)75695-4
  19. Lindgren, S., K. Petterson, A. Kaspersson, A. Jonsson, and P. Lingvall. 1985. Microbial dynamics during aerobic deterioration of silages. J. Sci. Food Agric. 36:765-774. https://doi.org/10.1002/jsfa.2740360902
  20. McDonald, P., A. R. Henderson, and S. J. E. Heron. 1991. The Biochemistry of Silage. 2nd edn. Chalcombe Publications, Bucks, England.
  21. Mills, J. A. and L. Kung, Jr. 2002. The effect of delayed ensiling and application of a propionic acid-based additive on the fermentation of barley silage. J. Dairy Sci. 85:1969-1975. https://doi.org/10.3168/jds.S0022-0302(02)74273-2
  22. Muck, R. E. 1993. The role of silage additives in making high quality silage. In: Silage Production: from Seed to Animal. Proceedings from the National Silage Production Conference, Syracuse, NY, USA. pp. 46-47.
  23. Muck, R. E. 2004. Effects of corn silage inoculants on aerobic stability. Trans. ASAE. 47:1011-1016. https://doi.org/10.13031/2013.16571
  24. Muck, R. E. and L. Kung, Jr. 1997. Effects of silage additives on ensiling. In: Silage: Field to Feedbunk. NRAES-99. Northeast Regional Agricultural Engineering Service, Ithaca, NY, USA. pp. 187-199.
  25. Ohyama, Y., S. Masaki, A. Takigawa, and T. Morichi. 1971. Studies on various factors affecting silage fermentation IX: Synergistic effect of inoculation of lactobacillus plantarum and addition of glucose on silage quality. Jap. J. Zootech. Sci. 42:1-8.
  26. Pitt, R. E., Y. Liu, and R. E. Muck. 1991. Simulation of the effect of additives on aerobic stability of alfalfa and corn silages. Trans. ASAE. 34:1633-1641. https://doi.org/10.13031/2013.31781
  27. Ranjit, N. K. and L. Kung, Jr. 2000. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J. Dairy Sci. 83:526-535. https://doi.org/10.3168/jds.S0022-0302(00)74912-5
  28. SAS Institute Inc. (2002). SAS/STAT User's Guide (Version 9). Cary, NC, USA.
  29. Sucu, E. and I. Filya. 2006. Effects of bacterial inoculants on fermentation, aerobic stability and rumen degradability characteristics of wheat silages. Turkish J. Vet. Anim. Sci. 30:187-193.
  30. Tilley, J. M. A. and R. A. Terry. 1963. A two-stage technique for the in vitro digestion of forage crops. Grass Forage Sci. 18:104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
  31. Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  32. Weinberg, Z. G. and R. E. Muck. 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiol. Rev. 19:53-68.
  33. Woolford, M. K. 1975. Microbiological screening of the straight chain fatty acids (C1-C12) as potential silage additives. J. Sci. Food Agric. 26:219-228. https://doi.org/10.1002/jsfa.2740260213
  34. Woolford, M. K. 1984. The Silage Fermentation. Marcel Dekker, Inc., New York, NY, USA.
  35. Zahiroddini, H., J. Baah, W. Absalom, and T. A. McAllister. 2004. Effect of an inoculant and hydrolytic enzymes on fermentation and nutritive value of whole crop barley silage. Anim. Feed Sci. Technol. 117:317-330. https://doi.org/10.1016/j.anifeedsci.2004.08.013
  36. Zahiroddini, H., J. Baah, and T. A. McAllister. 2006. Effects of microbial inoculants on the fermentation, nutrient retention, and aerobic stability of barley silage. Asian Australas. J. Anim. Sci. 19:1429-1436. https://doi.org/10.5713/ajas.2006.1429

Cited by

  1. Microbiological and chemical profiles of elephant grass inoculated with and without Lactobacillus plantarum and Pediococcus acidilactici pp.1432-072X, 2017, https://doi.org/10.1007/s00203-017-1447-1
  2. L.) straw on the Tibetan Plateau pp.17446961, 2017, https://doi.org/10.1111/grs.12191
  3. Isolation and molecular identification of lactic acid bacteria from King grass and their application to improve the fermentation quality of sweet Sorghum vol.34, pp.1, 2018, https://doi.org/10.1007/s11274-017-2387-2
  4. Effect of microbial inoculants on fermentation quality and aerobic stability of sweet potato vine silage vol.31, pp.12, 2018, https://doi.org/10.5713/ajas.18.0264
  5. L.) in Tibet vol.73, pp.3, 2018, https://doi.org/10.1111/gfs.12363
  6. The changes in dominant lactic acid bacteria and their metabolites during corn stover ensiling vol.125, pp.3, 2018, https://doi.org/10.1111/jam.13914
  7. A Comparison of Rice Straw and Whole-Crop Barley (Hordeum vulgare L.) Silage Supplements on Performance and Carcass Characteristics of Hanwoo (Bos taurus coreanae) Steers vol.10, pp.21, 2015, https://doi.org/10.3390/app10217725
  8. A Preliminary Study on Effects of Fermented Feed Supplementation on Growth Performance, Carcass Characteristics, and Meat Quality of Hanwoo Steers during the Early and Late Fattening Period vol.11, pp.11, 2015, https://doi.org/10.3390/app11115202