Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells

  • Jeong, Kyu-Tae (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry) ;
  • Lee, Eujin (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry) ;
  • Park, Na-Young (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Sun-Gun (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry) ;
  • Park, Hyo-Hyun (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry) ;
  • Lee, Jiean (R&D Center, Morechem Co., Ltd.) ;
  • Lee, Youn Ju (School of Medicine, Catholic University of Daegu) ;
  • Lee, Eunkyung (Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry)
  • Received : 2015.02.25
  • Accepted : 2015.06.17
  • Published : 2015.09.01
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Abstract

Imperatorin has been known to exert many biological functions including anti-inflammatory activity. In this study, we investigated the inhibitory effects of imperatorin on the production of inflammatory mediators in mouse bone marrow-derived mast cells (BMMC). Imperatorin inhibited degranulation and the generation of eicosanoids (leukotriene $C_4$ ($LTC_4$) and prostaglandin $D_2$ ($PGD_2$) in IgE/antigen (Ag)-stimulated BMMC. To elucidate the molecular mechanism involved in this process, we investigated the effect of imperatorin on intracellular signaling in BMMC. Biochemical analyses of the IgE/Ag-mediated signaling pathway demonstrated that imperatorin dramatically attenuated degranulation and the production of 5-lipoxygenase-dependent $LTC_4$ and cyclooxygenase-2-dependent $PGD_2$ through the inhibition of intracellular calcium influx/phospholipase $C{\gamma}1$, cytosolic phospholipase $A_2$/mitogen-activated protein kinases and/or nuclear factor-${\kappa}B$ pathways in BMMC. These results suggest that the effects of imperatorin on inhibition of degranulation and eicosanoid generation through the suppression of multiple steps of IgE/Ag-mediated signaling pathways would be beneficial for the prevention of allergic inflammation.

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References

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