한국식품저장유통학회지 (Food Science and Preservation)

  • 제22권2호
  • /
  • Pages.281-289
  • /
  • 2015
  • /
  • 3022-5477(pISSN)
  • /
  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
권호 표지
DOI QR코드

DOI QR Code

청국장 식이 보충이 당뇨 쥐의 산화적 스트레스와 항산화 영양소 함량에 미치는 영향

Effect of Chungkukjang supplementation on oxidative stress and antioxidant nutrients of diabetic rats induced by streptozotocin

  • 박현숙 (대구공업대학교 식음료계열) ;
  • 공현주 (대구한의대학교 대학원 한방식품학과) ;
  • 이언희 (영남대학교 대학원 식품영양학과) ;
  • 최은미 (경북대학교 대학원 식품영양학과) ;
  • 장정현 (동의과학대학교 미용계열) ;
  • 이명희 (송호대학교 뷰티케어과) ;
  • 홍주연 (대구한의대학교 한방식품조리영양학부) ;
  • 황수정 (대구한의대학교 한방식품조리영양학부) ;
  • 정현아 (대구한의대학교 한방식품조리영양학부) ;
  • 양경미 (대구한의대학교 한방식품조리영양학부)
  • Park, Heyun-Sook (Division of Hotel Culinary Arts, Daegu Technical College) ;
  • Kong, Hyun-Joo (Department of Herbal Food Science, Graduate School of Daegu Haany University) ;
  • Lee, Eon-Hee (Department of Food and Nutrition, Graduate School, Yeungnam University) ;
  • Choi, Eun-Mi (Department of Food Science and Nutrition, Graduate School, Kyungpook National University) ;
  • Jang, Joung-Hyeon (Division of Cosmetology, Dong-Eui Institute of Technology) ;
  • Lee, Myoung-Hee (Department of beauty care, Songho college) ;
  • Hong, Ju-Yeon (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Hwang, Su-Jung (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Jung, Hyeon-A (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Yang, Kyung-Mi (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University)
  • 투고 : 2014.09.16
  • 심사 : 2015.02.12
  • 발행 : 2015.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 산화적 스트레스에 대한 당뇨병의 예방과 관리에 대한 청국장과 블랙푸드의 효과를 평가하고자 대두청국장, 약콩청국장 그리고 블랙푸드를 첨가한 약콩청국장 분말을 식이에 첨가하여 STZ로 유도된 당뇨쥐에게 7주간 급여한 후 지질과산화물 함량, 항산화 효소 및 항산화 영양소 상태를 측정하였다. 그 결과 당뇨로 인하여 혈청과 간조직 내 증가된 지질과산화물 함량은 대두 및 블랙푸드가 첨가된 약콩청국장 공급군에서 p<0.05 수준에서 유의적으로 낮았다. 항산화 효소 중에서 당뇨 유도로 혈청 내 catalase 활성은 변화 없었으나 SOD 활성은 감소되었으며, 이때 약콩청국장의 공급으로 SOD 활성이 유도되었다. 항산화 영양소 중에서 혈청 내 retinol 함량은 당뇨 유도로 낮아졌지만 약콩청국장의 공급으로 7.5배 가량의 증가 현상을 보였고, 간 조직 내 retinol과 ${\alpha}$-tocopherol 함량은 세종류의 청국장의 공급으로 높일수 있었다. 당뇨 유도로 낮아진 간 조직 내의 GSH/GSSG와 비장 조직의 anthocyanin 함량은 약콩청국장, 그리고 혈청 내 총 항산화능은 블랙푸드 청국장의 공급으로 높아진 것으로 나타났다. 이상의 결과 당뇨병이 유발되었을 때 산화적 스트레스 지표인 지질과산화물 함량은 증가된 반면에, 항산화 효소인 catalase 및 SOD 활성과 항산화 영양소인 retinol, ${\alpha}$-tocopherol, GSH/GSSG, TAC 및 anthocyanin 함량이 낮아졌다. 그러나 세종류의 청국장이 당뇨쥐의 산화적 스트레스 반응과 항산화 영양소 상태에 긍정적인 반응 효과를 보였다. 이를 기반으로 향후 당뇨병의 예방과 관리를 위한 건강 기능성 식품으로서 청국장 및 블랙푸드의 활용 방안에 대한 좀 더 많은 연구가 이루어져야 하겠다.

This study was conducted to examine the effects of a supplemented Chungkukjang diet on oxidative stress and antioxidant nutrients in Streptozotocin (STZ, 45 mg/kg of BW, IP injection)-induced diabetic rats. Diets that contained soybean Chungkukjang powder (SC), Yakkong Chungkukjang powder (YC), and Yakkong Chungkukjang powder with black food added (YCB) were administered to the STZ-induced diabetic rats for seven weeks. The increased lipid peroxide contents of their serum and liver were slightly controlled by providing them three types of Chungkukjang. The retinol level in the serum was 7.5 times higher in the STZ-induced diabetic group after the provision of YC. The total antioxidant capacity (TAC) level in the serum was higher in the STZ-induced diabetic group after the provision of YCB. Also, the retinol and tocopherol levels in the liver of the STZ-induced diabetic rats increased after they were provided YCB, and the decreased reduced glutathione (GSH) /oxidized glutathione (GSSG) level in their liver improved after they were fed a diet that contained YC. Moreover, the decreased anthocyanin level in the liver of the STZ-induced diabetic group improved after the provision of three types of Chungkukjang powder. These findings suggest that the Chungkukjang diet is a valuable food for the management of the health of diabetic patients and for the prevention of diabetic complications.

키워드

참고문헌

  1. Park HS, Jang YJ, Choi DS, Namgung MA, Lee YJ, Jang SA (1995) Increased oxidative stress in Sciatic nerves of streptozotocin induced diabetic rats : lack of vitamin C effect. Diabetes, 19, 279-286
  2. Park SK, Park NY, Lim YS (2009) Effects of short term antioxidant cocktail supplementation on the oxidative stress and inflammatory response of renal inflammation in diabetic mice. Korean J Nutr, 42, 673-681 https://doi.org/10.4163/kjn.2009.42.8.673
  3. Atkinson MA, Maclaren NK (1994) The pathogenesis of insulin-dependent diabetes mellitus. N Engl J Med, 331, 1428-1436 https://doi.org/10.1056/NEJM199411243312107
  4. Behr GA, Silva EG, Ferreira AR, Cerski CT, Dal-Pizzol F, Moreira JC (2008) Pancreas beta-cells morphology, liver antioxidant enzymes and liver oxidative parameters in alloxan-resistant and alloxan-susceptible Wistar rats : a viable model system for the study of concepts into reactive oxygen species. Fundam Clin Pharmacol, 22, 657-666 https://doi.org/10.1111/j.1472-8206.2008.00628.x
  5. Santini SA, Marra G, Giardina B, Cotroneo P, Mordente, A, Martorana GE, Manto A, Ghirlanda G (1997) Defective plasma antioxidant defense and enhanced susceptability to lipid peroxidant in uncomplicated IDDM. Diabetes, 46, 1853-1858 https://doi.org/10.2337/diab.46.11.1853
  6. Akitha MK, Gondi M, Sakthivelu G, Giridhar P, Rajasekaran T, Ravishankar GA (2009) Functional attributes of soybean seeds and products, with reference to isoflavone content and antioxidant activity. Food Chem, 114, 771-776 https://doi.org/10.1016/j.foodchem.2008.10.011
  7. Joo EY, Park CS (2010) Antioxidative and fibrinolytic activity of extracts from soybean and Chungkukjang(fermented soybeans) prepared from a black soybean cultivar. Korean J Food Preserv, 17, 874-880
  8. Ramirez-Tortosa C, Andersen OM, Gardner PT, Morrice PC, Wood SG, Duthie SJ, Collins AR, Duthie GG (2001) Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E depleted rats. Free Radic Biol Med, 31, 1033-1037 https://doi.org/10.1016/S0891-5849(01)00618-9
  9. Jung MG, Lim JD (2012) Antioxidant, anticancer and immune activation of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Korean J Medicinal Crop Sci, 20, 259-269 https://doi.org/10.7783/KJMCS.2012.20.4.259
  10. Myung JE, Hwang IK (2008) Functional components and antioxidative activities of soybean extracts. Korean Soybean Digest, 25, 23-29
  11. Wei H, Cai Q, Rahn R (1996) Inhibition of UV light and fenton reaction-induced oxidative DNA damage by the soybean isoflavone genistein. Carcinogenesis, 17, 73-78 https://doi.org/10.1093/carcin/17.1.73
  12. Lee MH, Park YH, Oh HS, Kwak TS (2002) Isoflavone content in soybean and its processed products. Food Sci Biotechnol, 34, 365-369
  13. Philip GR, Forrest HN, George CF (1999) AIN-93 purified diets for laboratory rodents : final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr, 123, 1939-1951
  14. Ohkwwa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  15. Aebi H (1974) Catalase. In : Methods of enzymatic analysis Begmeyer HU editor. Verlag Chemie Academic Press. New York USA, p 673-684
  16. Bieri JG, Tolliver JJ, Catignani GL (1979) Simultaneous determination of ${\alpha}$-tocopherol and retinol in plasma or red cell by high pressure liquid chromatography. J Clin Nutr, 32, 2143-2148
  17. Furr HC, Amedee-Manesme O, Olson JA (1984) Gradient reversed-phased high-performance liquid chromatographic separation of naturally occurring retinoids. J Chromato, 309, 299-307 https://doi.org/10.1016/0378-4347(84)80037-7
  18. Theodrous PM, Helmut S (1981) Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Method Enzymology, 77, 373-382 https://doi.org/10.1016/S0076-6879(81)77050-2
  19. Park YE, Cho JH, Cho HM, Yi JY, Seo HW, Choung MG (2009) A new potato cultivar "Hongyoung", with red skin and flesh color, and high concentrations of anthocyanins. Korean J Breed Sci, 41, 502-506
  20. Fuleki T, Francis FJ (1968) Quantitative methods for anthocyanins. 2. Determination of total anthocyanin and degradation index for cranberry juice. J Food Sci, 33, 78-82 https://doi.org/10.1111/j.1365-2621.1968.tb00888.x
  21. Freitas JP, Filipe PM, Rodrigo FG (1997) Lipid peroxidation in type 2 normolipidemic diabetic patients. Diabetes Res Clin Prac, 36, 71-76 https://doi.org/10.1016/S0168-8227(97)00032-6
  22. Havel PJ, Uriu-Hare JY, Liu T, Stanhope KL, Stern JS, Keen CL, Ahren BO (1998) Marked and rapid decrease of circulating leptin in streptozotocin diabetic rats : reversal by insulin. Am J Physiol, 274, R1482-R1491
  23. Yan SD, Schmidt AM, Anderson CM, Zhang J, Brett J, Zou YS, Pinsky D, Stern D (1994) Enhanced cellular oxidant stress by the interaction of advanced glycation end products with their receptor/binding protein. J Biol Chem, 269, 9889-9897
  24. Arai K, Maguchi S, Fujii S, Ishibashi H, Oikawa K, Taniguchi N (1987) Glycation and inactivation of human Cu-Zn-superoxide dismutase. J Biol Chem, 262, 16969-16972
  25. KIm SS, Son SM (2008) Oxidative stress and cell dysfunction in diabetes : role of ROS produced by mitochondria and NAD(P)H oxidase. Korean Diabetes J, 32, 389-398 https://doi.org/10.4093/kdj.2008.32.5.389
  26. Sundaram RK, Bhaskar A, Vijayalingam S, Viswanthan M, Mohan R, Shanmugasundaram KR (1996) Antioxidant status and lipid peroxidation in type II diabetes mellitus with and without complication. Clin Sci, 90, 255-260 https://doi.org/10.1042/cs0900255
  27. Dave GS, Kalia K (2001) Hyperglycemia induced oxidative stress in type-1 and type-2 diabetic patients with and without nephropathy. Cell Mol Biol, 53, 68-78
  28. Ceriello A, Bortolotti N, Falleti E, Taboga C, Tonutti L, Crescentini A, Motz E, Lizzio S, Russo A, Bartoli E (1997) Total radical trapping antioxidant parameter in NIDDM patients. Diabetes Care, 20, 194-197 https://doi.org/10.2337/diacare.20.2.194
  29. Seo JS, Yang KM, Choi MJ (1995) Effects of dietary vitamin A on the status of antioxidants in ethanol-treatedrats. J Korean Soc Food Nutr, 24, 848-858
  30. Videla LA (1984) Chemically induced antioxidantsenstive respiration. Relation to glutathione content and lipid peroxidation in the perfused rat liver. Fed Eur Biochem Soc, 178, 119-121 https://doi.org/10.1016/0014-5793(84)81253-3
  31. Burk RF, Trumble MJ, Lawrance RA (1980) Rat hepatic cytosolic glutathione-dependent enzyme protection against lipid peroxidation in the NADPH-microsomal lipid peroxidation-system. Biochem Biophys Acta, 618, 35-41 https://doi.org/10.1016/0005-2760(80)90051-X
  32. Opara EC, Abdel-Rahman E, Ssoliman S, Kamel WA, Souka S, Lowe JE, Abdel-Aaleem S (1999) Depletion of total antioxidant capacity in type 2 diabetes. Metabolism, 48, 1414-1417 https://doi.org/10.1016/S0026-0495(99)90152-X
  33. Kim JH, Kim MJ (2004) Dietary intakes and plasma antioxdant vitamins levels in Korean elderly with diabetes. Asia Pac J CLin Nutr, 13, S152
  34. Kim YH, Yun HT, Park KY (2004) Biological effects of black colored soybean. Plant Resources, 7, 195-199
  35. Ryu SH (2001) Studies on antioxidative effects and antioxidative componets of soybean and Chongkukjang. Ph D Thesis, Inje University, Korea, p 46-47

피인용 문헌

  1. Antioxidant Effect of Chungkukjang Supplementation against Memory Impairment induced by Scopolamine in Mice vol.26, pp.3, 2016, https://doi.org/10.17495/easdl.2016.6.26.3.237