Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Phenolic Compounds from the Leaves of Stewartia pseudocamellia Maxim. and their Whitening Activities

  • Roh, Hyun Jung (Natural Product Laboratory, School of Pharmacy, Sungkyunkawn University) ;
  • Noh, Hye-Ji (Lifetree Biotech Co. Ltd.) ;
  • Na, Chun Su (Lifetree Biotech Co. Ltd.) ;
  • Kim, Chung Sub (Natural Product Laboratory, School of Pharmacy, Sungkyunkawn University) ;
  • Kim, Ki Hyun (Natural Product Laboratory, School of Pharmacy, Sungkyunkawn University) ;
  • Hong, Cheol Yi (Lifetree Biotech Co. Ltd.) ;
  • Lee, Kang Ro (Natural Product Laboratory, School of Pharmacy, Sungkyunkawn University)
  • Received : 2014.12.11
  • Accepted : 2015.01.15
  • Published : 2015.05.01
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Abstract

The half-dried leaves of Stewartia. pseudocamellia were extracted with hot water (SPE) and partitioned with n-hexane (SPEH), dichloromethane (SPED), and ethyl acetate (SPEE) successively. SPE and SPEE showed significant inhibitory effects against melanogenesis and tyrosinase activities. By bioassay-guided isolation, ten phenolic compounds were isolated by column chromatography from SPEE. The whitening effect of the isolated compounds from SPEE were tested for the inhibitory activities against melanogenesis using B16 melanoma cells, in vitro inhibition of tyrosinase, and L-3,4-dihydorxy-indole-2-carboxylic acid (L-DOPA) auto-oxidation assay. A cytotoxic activity assay was done to examine the cellular toxicity in Raw 264.7 macrophage cells. Of the compounds isolated, gallic acid and quercetin revealed significant inhibitory activities against melanogenesis compared to arbutin. In particular, quercetin exhibited similar inhibitory activities against tyrosinase and L-DOPA oxidation without cytotoxicity. These results suggested that SPE could be used as a potential source of natural skin-whitening material in cosmetics as well as in food products.

Keywords

References

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