생태와환경 (Korean Journal of Ecology and Environment)

  • 제48권2호
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  • Pages.86-94
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  • 2015
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  • 2288-1115(pISSN)
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  • 2288-1123(eISSN)
한국수생태학회 (The Korean Society of Limnology)
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실내종 Chironomus riparious와 야외종 Chironomus plumosus의 중금속 노출에 따른 분자지표 유전자 발현

The Molecular Biomarker Genes Expressions of Rearing Species Chironomus riparious and Field Species Chironomus plumosus Exposure to Heavy Metals

  • Kim, Won-Seok (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Kim, Rosa (Korea Institute of Toxicology) ;
  • Park, Kiyun (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Chamilani, Nikapitiya (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Kwak, Ihn-Sil (Department of Fisheries and Ocean Science, Chonnam National University)
  • 투고 : 2015.01.20
  • 심사 : 2015.06.05
  • 발행 : 2015.06.30
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초록

Chironomous is aquatic insect belonging to order Diptera, family Chironomidae. Their larval stage can be found mainly in aquatic benthic environment, hence good model organism to study environmental toxicology assessments and consider as useful bio indicators of contamination of the aquatic environment. In this study, Chironomus Heat Shock Proteins, Cytochrome 450, Glutathione S-transferase, Serine-type endopeptidase gene expressions were compared between polluted field areas (Chironomus plumosus) and under laboratory conditions (Chironomus riparious) to investigate molecular indicators for environmental contaminant stress assessment. Heavy metal (Al, Fe, Mn, Cu, Cr, Zn, Se, Pb, As, Cd) concentrations in sediments collected from three study areas exceeded the reference values. Moreover, HSPs, CYP450 and GST gene expression except SP for C. plumosus showed higher expression than C. riparious gene expression. Similar gene expression pattern was observed in C. riparious that exposed environment waters up to 96 h when compared to C. plumosus exposed to waters that grown in lab conditions. In summary, this comparative gene expression analysis in Chironomous between field and laboratory condition gave useful information to select candidate molecular indicators in heavy metal contaminations in the environment.

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참고문헌

  1. Agradi, E., R. Baga, F. Cillo, S. Ceradini and D. Heltai. 2000. Environmental contaminants and biochemical response in eel exposed to Po river water. Chemosphere 41: 1555-1556. https://doi.org/10.1016/S0045-6535(00)00067-9
  2. Andreia, C.M.R., G. Carlos, Q. Carla, G. Oksana, Z. Vladimir, B. Carlos, M.V.M.S. Amaden and L.T.P. Joao. 2015. Life history and biochemical effects of Chlorantraniliprole on Chironomus riparious. Science of the Total Environment 508: 506-513. https://doi.org/10.1016/j.scitotenv.2014.12.021
  3. Bian, G., A.S. Raikhel and J. Zhu. 2008. Characterization of a juvenile hormone-regulated chymotrypsin-like serine protease gene in Aedes aegypti mosquito. Insect Biochemistry and Molecular Biology 38: 190-200. https://doi.org/10.1016/j.ibmb.2007.10.008
  4. Buhler, D.R. and D.E. Williams. 1988. The role of biotrasnformation in the toxicity of chemicals. Aquatic Toxicology 11: 19-28. https://doi.org/10.1016/0166-445X(88)90004-5
  5. Contardo-Jara, V. and C. Wiegand. 2008. Biotransformation and antioxidant enzymes of Lumbriculus variegates as biomarkers of contaminated sediment exposure. Chemosphere 70: 1879-1888. https://doi.org/10.1016/j.chemosphere.2007.07.072
  6. Demirak, A., F. Yilmaz, A.L. Tuna and N. Ozdemir. 2006. Heavy metals in water, sediment and tissues of Leuciscus cephalus from a stream in southwestern Turkey. Chemosphere 63: 1451-1458. https://doi.org/10.1016/j.chemosphere.2005.09.033
  7. Faria, M., D. Huertas, D.X. Soto, J.O. Grimalt, J. Catalan, M.C. Riva and C. Barata. 2010. Contaminant accumulation and multi-biomarker responses in field collected zebra mussels (Dreissena polymorpha) and crayfish (Procambarus clarkii), to evaluate toxicological effects of industrial hazardous dumps in the Ebro river (NE Spain). Chemosphere 78: 232-240. https://doi.org/10.1016/j.chemosphere.2009.11.003
  8. Faria, M.S., R.J. Lopes, J. Malcato, A.J. Nogueira and A.M. Soares. 2008. In situ bioassays with Chironomus riparius larvae to biomonitor metal pollution in rivers and to evaluate the efficiency of restoration measures in mine areas. Environmental Pollution 151: 213-221. https://doi.org/10.1016/j.envpol.2007.01.050
  9. Gagne, F., C. Blaise and J. Hellou. 2004. Endocrine disruption and health effects of caged mussels, Elliptio complanata, placed downstream from a primary-treated sewage municipal effluent plume for 1 year. Comparative Biochemistry and Physiology Part C 138: 33-44. https://doi.org/10.1016/j.cbpb.2004.02.014
  10. Garcia Sampaio, F., C. de Lima Boijink, E. Tie Oba, L. Romagueira Bichara dos Santos, A. Lucia Kalinin and F. Tadeu Rantin. 2008. Antioxidant defenses and biochemical changes in pacu (Piaractus mesopotamicus) in response to single and combined copper and hypoxia exposure. Comparative Biochemistry and Physiology Part C 147: 43-51.
  11. George, D., N. Ferry, E.J. Back and A.M. Gatehouse. 2008. Characterisation of midgut digestive proteases from the maize stem borer Busseola fusca. Pest Management Science 64: 1151-1158. https://doi.org/10.1002/ps.1610
  12. Hightower. 1991. Heat shock, stress proteins, chaperones, and proteotoxicity. Cell 66: 191-197. https://doi.org/10.1016/0092-8674(91)90611-2
  13. Huang, Q., Y. Chen, Y. Chi, Y. Lin, H. Zhang, C. Fang and S. Dong. 2015. Immunotoxic effects of perfluorooctane sulfonate and di(2-ethylhexyl) phthalate on the marine fish Oryzias melastigma. Fish & Shellfish Immunology 44: 302-306. https://doi.org/10.1016/j.fsi.2015.02.005
  14. Kalmar, B. and L. Greensmith. 2009. Induction of heat shock proteins for protection against oxidative stress. Advanced Drug Delivery Reviews 61: 310-318. https://doi.org/10.1016/j.addr.2009.02.003
  15. Kilemade, M., M.G. Hartl, J. O'Halloran, N.M. O'Brien, D. Sheehan, C. Mothersill and F.N. van Pelt. 2009. Effects of contaminated sediment from Cork Harbour, Ireland on the cytochrome P450 system of turbot. Ecotoxicology and Environmental Safety 72(3): 747-755. https://doi.org/10.1016/j.ecoenv.2008.09.026
  16. Kim, M., K. Park, J.Y. Park and I.S. Kwak. 2013. Heavy metal contamination and metallothionein mRNA in blood and feathers of Black-tailed gulls (Larus crassirostris) from South Korea. Environmental Monitoring and Assessment 185: 2221-2230. https://doi.org/10.1007/s10661-012-2703-0
  17. Limon-Pacheco, J. and M.E. Gonsebatt. 2009. The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutation Research 674: 137-147. https://doi.org/10.1016/j.mrgentox.2008.09.015
  18. Lopes, P.A., T. Pinheiro, M.C. Santos, M.D.L. Mathias, M.J. Collares-Pereira and A.M. Viegas-Crespo. 2001. Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides complex) to inorganic pollutants exposure. Science of the Total Environment 280: 153-163. https://doi.org/10.1016/S0048-9697(01)00822-1
  19. Maria, V.L., A.C. Correia and M.A. Santos. 2003. Genotoxic and hepatic biotransformation responses induced by the overflow of pulp and secondary-treated effluents on Aguilla anguilla L. Ecotoxicology and Environment Safety 55: 126-137. https://doi.org/10.1016/S0147-6513(02)00098-2
  20. Martinez-Paz, P., M. Morales, J.L. Martinez-Guitarte and G. Morcillo. 2012. Characterization of a cytochrome P450 gene (CYP4G) and modulation under different exposures to xenobiotics (tributyltin, nonylphenol, bisphenol A) in Chironomus riparius aquatic larvae. Comparative Biochemistry and Physiology Part C 155(2): 333-343. https://doi.org/10.1016/j.cbpc.2011.10.001
  21. Michailova, P., J. Ilkova and K.N. White. 2003. Functional and structural rearrangements of salivary gland polyene chromosomes of Chironomus riparius Mg. (Diptera,Chironomidae) in response to freshly neutralized aluminum. Environmental Pollution 123(2): 193-207. https://doi.org/10.1016/S0269-7491(02)00405-0
  22. Ministry of Maritime Affairs and Fisheries. 2005. Maritime environment Pollutant' Testing method. p. 238-243.
  23. Monica, M., M. Pedro, M. Raquel, P. Rosario, U. Josune, M. Jose and M. Gloria. 2014. Transcriptional changes induced by in vivo exposure to Pentachlorophenol(PCP) in Chironomus riparious (Diptera) aquatic larvae. Aquatic Toxicology 157: 1-9. https://doi.org/10.1016/j.aquatox.2014.09.009
  24. Nadella, S.R., J.L. Fitzpatrick, N. Franklin, C. Bucking, S. Smith and C.M. Wood. 2009. Toxicity of dissolved Cu, Zn, Ni and Cd to developing embryos of the blue mussel (Mytilus trossolus) and the protective effect of dissolved organic carbon. Comparative Biochemistry and Physiology Part C 149: 340-348.
  25. Nair, P.M. and J. Choi. 2011. Identification, characterization and expression profiles of Chironomus riparius glutathione S-transferase (GST) genes in response to cadmium and silver nanoparticles exposure. Aquatic Toxicology 101(3-4): 550-560. https://doi.org/10.1016/j.aquatox.2010.12.006
  26. OECD. 2001. Organization for Economic Cooperation and Development, Guideline for testing of chemicals, sediment-water chironomid toxicity test using spiked sediment. p. 218.
  27. Park, K. and I.S. Kwak. 2008. Expression of Chironomus riparius serine-type endopeptidase gene under di-(2-ethylhexyl)-phthalate (DEHP) exposure. Comparative Biochemistry and Physiology Part B 151(3): 349-354. https://doi.org/10.1016/j.cbpb.2008.08.004
  28. Park, K. and I.S. Kwak. 2009. Alcohol dehydrogenase gene expression in Chironomus riparius exposed to di(2-ethylhexyl) phthalate. Comparative Biochemistry and Physiology Part C 150(3): 361-367. https://doi.org/10.1016/j.cbpc.2009.05.015
  29. Park, K. and I.S. Kwak. 2010. Molecular effects of endocrinedisrupting chemicals on the Chironomus riparius estrogen-related receptor gene. Chemosphere 79(9): 934-941.
  30. Park, K. and I.S. Kwak. 2014a. Characterization and gene expression of heat shock protein 90 in marine crab Charybdis japonica following bisphenol A and 4-nonylphenol exposures. Environmental Health and Toxicology 29: 1-7.
  31. Park, K. and I.S. Kwak. 2014b. The effect of temperature gradients endocrine signaling and antioxidant gene expression during Chironomus riparious development. Science of the Total Environment 471: 1003-1011.
  32. Park, K., H.W. Bang, J. Park and I.S. Kwak. 2009. Ecotoxicological multilevel-evaluation of the effects of fenbendazole exposure to Chironomus riparius larvae. Chemosphere 77(3): 359-367. https://doi.org/10.1016/j.chemosphere.2009.07.019
  33. Park, K., J. Park, J. Kim and I.S. Kwak. 2010. Biological and molecular responses of Chironomus riparius (Diptera, Chironomidae) to herbicide 2,4-D (2,4-dichlorophenoxyacetic acid). Comparative Biochemistry and Physiology Part C 151(4): 439-446. https://doi.org/10.1016/j.cbpc.2010.01.009
  34. Park, K., J.S. Lee, J. Kang, J.W. Kim and I.S. Kwak. 2015. Cascading effects from survival to physiological activities, and gene expression of heat shock protein 90 on the abalone Haliotis discus hannai responding to continuous thermal stress. Fish and shellfish Immunology 42: 233-240. https://doi.org/10.1016/j.fsi.2014.10.036
  35. Rainbow, P.S. 2002. Trace metal concentrations in aquatic invertebrates: why and so what?. Environmental Pollution 120: 497-507. https://doi.org/10.1016/S0269-7491(02)00238-5
  36. Rand, G.M., P.G. Wells and L.S. McCarty. 2003. Introduction to aquatic toxicology. In: Rand GM (ed) Fundamentals of aquatic toxicology. Taylor and Francis, New York. p. 123-167.
  37. Rios-Sicairos, J., M. Betancourt-Lozano, B. Leal-Tarin, R. Hernandez-Cornejo, G. Aguilar-Zarate, L.M. Garcia-De-La- Parra, J.N. Gutierrez, F. Marquez-Rocha and A. Garcia-Gasca. 2010. Heat-shock protein (Hsp70) and cytochrome P-450 (CYP1A) in the white mullet Mugil curema (Pisces: Mugilidae) as biomarkers to assess environmental quality in coastal lagoons. Journal of Environmental Science and Health Part A 45(1): 68-74. https://doi.org/10.1080/10934520903388855
  38. Rocha-e-Silva, T.A., F.T. Rantin, J.E. Matsumura-Tundisi, T. Matsumura-Tundisi, J.G. Tundisi and I.A. Degterev. 2004. Dynamics of cytochrome P450 inducers in polluted sites of Sao Paulo city reservoirs. Ecotoxicology and Environmental Safety 59(1): 109-115. https://doi.org/10.1016/S0147-6513(03)00097-6
  39. Shu, Y., Y. Du and J. Wang. 2011. Molecular characterization and expression patterns of Spodoptera litura heat shock protein 70/90, and their response to zinc stress. Comparative biochemistry and physiology Part A 158(1): 102-110. https://doi.org/10.1016/j.cbpa.2010.09.006
  40. Terra, W.R., B.P. Jordao and R.J. Dillon. 1996. Digestive enzymes. In: Bilingsley, P., M. Lehane, (eds.), Biologyof the Insect Midgut. Chapman and Hall, London. p. 159-194.
  41. Tuikka, A.I., C. Schmitt, S. Hoss, N. Bandow, P.C. von der Ohe, D. de Zwart, E. de Deckere, G. Streck, S. Mothes, B. van Hattum, A. Kocan, R. Brix, W. Brack, D. Barcelo, A.J. Sormunen and J.V. Kukkonen. 2011. Toxicity assessment of sediments from three European river basins using a sediment contact test battery. Ecotoxicology and Environmental Safety 74: 123-131. https://doi.org/10.1016/j.ecoenv.2010.08.038
  42. Volpicella, M., J. Cordewener, M.A. Jongsma. R. Gallerani, L.R. Ceci and J. Beekwilder. 2006. Identification and characterization of digestive serine proteases from inhibitorresistant Helicoverpa zea larval midgut. Journal of Chromatography B 833: 26-32. https://doi.org/10.1016/j.jchromb.2005.10.021