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http://dx.doi.org/10.11614/KSL.2015.48.2.086

The Molecular Biomarker Genes Expressions of Rearing Species Chironomus riparious and Field Species Chironomus plumosus Exposure to Heavy Metals  

Kim, Won-Seok (Department of Fisheries and Ocean Science, Chonnam National University)
Kim, Rosa (Korea Institute of Toxicology)
Park, Kiyun (Department of Fisheries and Ocean Science, Chonnam National University)
Chamilani, Nikapitiya (Department of Fisheries and Ocean Science, Chonnam National University)
Kwak, Ihn-Sil (Department of Fisheries and Ocean Science, Chonnam National University)
Publication Information
Korean Journal of Ecology and Environment / v.48, no.2, 2015 , pp. 86-94 More about this Journal
Abstract
Chironomous is aquatic insect belonging to order Diptera, family Chironomidae. Their larval stage can be found mainly in aquatic benthic environment, hence good model organism to study environmental toxicology assessments and consider as useful bio indicators of contamination of the aquatic environment. In this study, Chironomus Heat Shock Proteins, Cytochrome 450, Glutathione S-transferase, Serine-type endopeptidase gene expressions were compared between polluted field areas (Chironomus plumosus) and under laboratory conditions (Chironomus riparious) to investigate molecular indicators for environmental contaminant stress assessment. Heavy metal (Al, Fe, Mn, Cu, Cr, Zn, Se, Pb, As, Cd) concentrations in sediments collected from three study areas exceeded the reference values. Moreover, HSPs, CYP450 and GST gene expression except SP for C. plumosus showed higher expression than C. riparious gene expression. Similar gene expression pattern was observed in C. riparious that exposed environment waters up to 96 h when compared to C. plumosus exposed to waters that grown in lab conditions. In summary, this comparative gene expression analysis in Chironomous between field and laboratory condition gave useful information to select candidate molecular indicators in heavy metal contaminations in the environment.
Keywords
Chironomus; heavy metal; molecular biomarker; Heat Shock Protein; Cytochrome P450; Glutathione S-transferase; Serine-type endopeptidase;
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