Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antimicrobial and Immunological activities of Vinca minor Extracts

빈카 마이너 추출물의 항균 및 면역활성 연구

  • Kim, Jun-Sub (Department of Biotechnology, Korea National University of Transportation) ;
  • Kang, Jo-Eun (Department of Biotechnology, Korea National University of Transportation) ;
  • Yu, Il-Hwan (Department of Biotechnology, Korea National University of Transportation) ;
  • Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation) ;
  • Moon, Gi-Seong (Department of Biotechnology, Korea National University of Transportation) ;
  • Lee, Hyang-Yeol (Department of Biotechnology, Korea National University of Transportation)
  • 김준섭 (한국교통대학교 생명공학과) ;
  • 강조은 (한국교통대학교 생명공학과) ;
  • 유일환 (한국교통대학교 생명공학과) ;
  • 정경환 (한국교통대학교 생명공학과) ;
  • 문기성 (한국교통대학교 생명공학과) ;
  • 이향렬 (한국교통대학교 생명공학과)
  • Received : 2015.03.05
  • Accepted : 2015.03.25
  • Published : 2015.03.30
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Abstract

Vinca alkaloid compounds including vincamine from Vinca minor L. have been extracted by ethanol and hot water extraction methods. Antimicrobial properties of those extracts have been investigated against dandruff causing microorganism Malassezia furfur, yeast, Gram positive and negative bacteria. Vincamine standard and ethanol extract showed no sign of antimicrobial activity, whereas hot-water extract had the activity against M. furfur. Furthermore hot water extract had antimicrobial activity against Gram positive Bacillus sp. and Gram negative Escherichia coli. Cytotoxic properties of those extracts have also been investigated with HaCaT cell (human keratinocyte), HT-29 cell (human colorectal adenocarcinoma cell) and Raw cell, showing no significant cytotoxic effects. We also measured the ROS using dichlorofluorescein diacetate (DCFDA), a popular fluorescence-based probe for reactive oxygen species detection. The result showed the increasement of reactive oxygen species formation (20%) in HaCaT and HT-29 cell lines.

EtOH 추출법과 열수 추출법을 사용하여 빈카민 등의 빈카 알칼로이드 화합물을 함유한 빈카 마이너 추출물을 얻었다. 이들 추출물을 사용하여 비듬균, 효모, 그람양성 및 음성세균에 대한 항균활성을 측정 하였다. 비듬균(Malassezia furfur)에 대하여 표준물질인 빈카민과 EtOH 추출물은 항균활성을 보이지 않은 반면 열수 추출물은 항균활성을 나타내었다. 또한 열수 추출물은 그람양성 및 음성세균에 속하는 바실러스(Bacillus sp.)와 대장균(Escherichia coli)에 대해서도 항균활성을 나타내었다. 추출물의 세포독성은 HaCaT 세포(인간 케라티노사이트 세포), HT-29 세포(인간 결직장 선종암 세포), Raw 세포(인간 대식세포)를 대상으로 측정하였으며, 해당 세포들에 대해 특별한 세포독성은 발견 되지 않았다. 또한 형광기반의 탐색물질인 디클로로플루오신 디아세테이트(DCFDA)를 이용한 활성산소종을 측정하였다. 그 결과 HaCaT 세포와 HT-29 세포군에서 활성산소종의 형성이 약 20% 정도 증가하는 것을 알 수 있었다.

Keywords

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