한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제32권1호
  • /
  • Pages.16-22
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  • 2015
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  • 1225-9098(pISSN)
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  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
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빈카 마이너 추출물에 함유된 알칼로이드들의 분광학적 분석 및 항산화 효능 연구

Antioxidant Effects and Spectrophotometry Analysis of Alkaloids from Vinca minor

  • 김준섭 (한국교통대학교 생명공학과) ;
  • 주지혜 (한국교통대학교 생명공학과) ;
  • 강조은 (한국교통대학교 생명공학과) ;
  • 장선동 (한국교통대학교 생명공학과) ;
  • 정경환 (한국교통대학교 생명공학과) ;
  • 문기성 (한국교통대학교 생명공학과) ;
  • 이향렬 (한국교통대학교 생명공학과)
  • Kim, Jun-Sub (Department of Biotechnology, Korea National University of Transportation) ;
  • Joo, Ji-Hye (Department of Biotechnology, Korea National University of Transportation) ;
  • Kang, Jo-Eun (Department of Biotechnology, Korea National University of Transportation) ;
  • Jang, Sun-Dong (Department of Biotechnology, Korea National University of Transportation) ;
  • Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation) ;
  • Moon, Gi-Seong (Department of Biotechnology, Korea National University of Transportation) ;
  • Lee, Hyang-Yeol (Department of Biotechnology, Korea National University of Transportation)
  • 투고 : 2015.03.05
  • 심사 : 2015.03.25
  • 발행 : 2015.03.30
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초록

빈카마이너 L.의 알칼로이드는 HPLC의 PDA 검출기를 이용하여 측정하고 이를 표준시료인 빈카민의 자외선/가시광선 흡수 스펙트럼과 비교하여 분석하였다. 플라보노이드류를 포함하여 추출물에서 9개 이상의 화합물에 대한 자외선/가시광선 흡수 스펙트럼을 얻었다. 추출방법에 따라 에탄올 추출물과 열수 추출물을 얻고 이를 DPPH 라디칼 소거법에 의해 항산화 효과를 조사하였다. 그 결과 에탄올 추출물과 열수 추출물 모두 비교군인 케르세틴과 비타민 C 대비 약 14~15%의 항산화 효과를 보여 추출법에 상관없이 유사한 결과를 보였다.

Alkaloids of Vinca minor L. were characterized by HPLC equipped with the photo diode array detector and compared their UV/vis absorption spectra with an index material such as vincamine. UV/vis absorption spectra of more than 9 compounds belong to various classes such as flavonoids were shown and characterized. Extracts with ethanol or hot water were tested against the DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical to investigate their antioxidant activity. Based on the results, those extracts show about 14-15% of antioxidant activity of quercetine and vitamin C used as standards.

키워드

참고문헌

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피인용 문헌

  1. Biological Effects of Vinca minor extract; Tyrosinase inhibition, stimulation of ROS generation and increasement of cell migration activity in keratinocytes vol.33, pp.4, 2016, https://doi.org/10.12925/jkocs.2016.33.4.788