Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Activation of Cryptic hop Genes from Streptomyces peucetius ATCC 27952 Involved in Hopanoid Biosynthesis

  • Ghimire, Gopal Prasad (Institute of Biomolecule Reconstruction (iBR), Department of BT-convergent Pharmaceutical Engineering, Sun Moon University) ;
  • Koirala, Niranjan (Institute of Biomolecule Reconstruction (iBR), Department of BT-convergent Pharmaceutical Engineering, Sun Moon University) ;
  • Sohng, Jae Kyung (Institute of Biomolecule Reconstruction (iBR), Department of BT-convergent Pharmaceutical Engineering, Sun Moon University)
  • Received : 2014.08.25
  • Accepted : 2014.11.18
  • Published : 2015.05.28
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Abstract

Genes encoding enzymes with sequence similarity to hopanoids biosynthetic enzymes of other organisms were cloned from the hopanoid (hop) gene cluster of Streptomyces peucetius ATCC 27952 and transformed into Streptomyces venezuelae YJ028. The cloned fragments contained four genes, all transcribed in one direction. These genes encode polypeptides that resemble polyprenyl diphosphate synthase (hopD), squalene-phytoene synthases (hopAB), and squalene-hopene cyclase (hopE). These enzymes are sufficient for the formation of the pentacyclic triterpenoid lipid, hopene. The formation of hopene was verified by gas chromatography/mass spectrometry.

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References

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