Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Gene expression in plant according to RNAi treatment of the tobacco whitefly

RNAi 기법으로 담배가루이 방제를 위해 선발된 유전자의 식물체내 발현

  • Kim, Jeong-Hee (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Jung-Kyu (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, Yong-Man (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Youn, Young-Nam (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 김정희 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 서은영 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 김정규 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 임현섭 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2015.06.16
  • Accepted : 2015.06.24
  • Published : 2015.06.30
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Abstract

Three genes selected from cDNA library of tobacco whitefly, Bemisia tabaci, were checked whether these genes expressed in plant or not, and confirmed the change of gene expression using qRT-PCR in the tobacco whitefly. First of all, three genes were inserted in Tobacco rattle virus (TRV) RNA2 vector using Sac I and Xho I restriction enzymes, and conducted agro-infiltration in tobacco plants (Nicotiana benthamianana). And then, it was confirmed that TRV RNA2 vector and genes inserted in TRV RNA2 vector were expressed in plant. So, after feeding the tobacco whitefly the plants inoculated the genes and induced RNAi of the genes, we plan to confirm the RNAi in the whitefly and investigate the changes of gene expression through the qRT-PCR.

Keywords

References

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