Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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Monitoring of VHS and RSIVD in cultured Paralichthys olivaceus of Jeju in 2014

2014년 제주도 양식 넙치, Paralichthys olivaceus에 대한 VHS 및 RSIVD 모니터링

  • PARK, Hyun-Kyung (Faculty of Marine Biomedical Science, Jeju National University) ;
  • KIM, Seung-Min (Faculty of Marine Biomedical Science, Jeju National University) ;
  • LEE, Da-Won (Faculty of Marine Biomedical Science, Jeju National University) ;
  • JUN, Lyu-Jin (Fish Vaccine Resarch Center, Jeju National University) ;
  • JEONG, Joon-Bum (Faculty of Marine Biomedical Science, Jeju National University)
  • 박현경 (제주대학교 해양의생명과학부) ;
  • 김승민 (제주대학교 해양의생명과학부) ;
  • 이다원 (제주대학교 해양의생명과학부) ;
  • 전려진 (제주대학교 수산백신연구센터) ;
  • 정준범 (제주대학교 해양의생명과학부)
  • Received : 2015.06.08
  • Accepted : 2015.06.19
  • Published : 2015.06.30
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Abstract

The outbreak of viral diseases caused by viral haemorrhagic septicaemia virus (VHSV) and red seabream iridovirus (RSIV) have been reported in cultured olive flounder, Paralichthys olivaceus. VHSV has been a serious viral disease that infects the olive flounders in South Korea. Clinical signs of VHSV infection are skin darkening, abdominal distension and haemorrhages. Outbreaks of fish iridoviral disease was first reported from red seabream, Pagrus major farms in Japan. Recently, iridovirus infection have occurred frequently from olive flounder farms in South Korea. In this study, disease surveillance was performed to monitor the prevalence of VHSV and RSIV in olive flounder in 2014. The samples were collected from 60 different olive flounder farms in Jeju from April, May, September, November and December in 2014. RT-PCR (VHSV) or PCR (RSIV) results showed that VHSV were detected in 5 farms, but RSIV has not been detected in any farms. The migration of olive flounder was restricted for the quarantine in 5 farms of VHS outbreak. The nucleocapsid protein (N) gene and glycoprotein (G) gene sequences of the 5 Korean VHSV isolates were successfully amplified and sequenced. Phylogenetic analysis was performed using the VHSV sequences reported here together comparison with the nucleotide sequences available from the GenBank database. Phylogenetic analysis indicated that most of Korea VHSV belong to the genotype IVa and closely related to the strains from Japan and China.

Keywords

References

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