Journal of the Korean Society of Radiology (한국방사선학회논문지)

The Korean Society of Radiology (한국방사선학회)
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Active Transport Characteristics of Anions through a Cell Membrane Model which Irradiated by γ-ray

감마선이 조사된 세포막모델을 통한 음이온의 능동 전달 특성

  • Ko, In-Ho (Dept. of Radiotechnology, Cheju Halla University) ;
  • Yeo, Jin-Dong (Dept. of Radiotechnology, Sorabol University)
  • 고인호 (제주한라대학교 방사선과) ;
  • 여진동 (서라벌대학교 방사선과)
  • Received : 2015.03.06
  • Accepted : 2015.06.25
  • Published : 2015.06.30
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Abstract

The active transport characteristics of anions of cell membrane model which irradiated by $^{60}Co\;{\gamma}-ray$ was investigated. The cell membrane model used in this experiment was a sulfonated copolymerized membrane of poly(1-methyl-4-vinylpyridiniumiodide-co-divinylbenzene : MeVP-DVBI). First, the initial flux of $OH^-$ and $Cl^-$, $Na^+$ of membrane which was not irradiated was decreased with increase of thickness of membrane $80-200{\mu}m$, increased with increase of NaOH concentration 0-0.5mol/L and MeVP-DVBI concentration 20-80% was increased with initial flux of $OH^-$ and $Cl^-$, decreased with initial flux of $Na^+$. Second, the initial flux of membrane which was irradiated was less than that. And the driving force of pH of irradiated membrane was significantly increased more than membrane which was not irradiated. The initial flux of the $OH^-$ ion was decreased with increase of $H^+$ ion concentration. As selective transport of $OH^-$ and $Cl^-$ of cell membrane model were abnormal, cell damages were appeared at cell.

감마선이 조사된 세포막 모델에서 음이온의 능동전달특성을 연구하였다. 이 실험에 사용된 세포막 모델은 세포막의 구형단백질, 당단백질 등의 기능을 지니고 있는 친수성의 극성인 poly(1-methyl-4-vinylpyridinium iodide-co-divinylbenzene: MeVP-DVBI)분말을 세포막의 인지질에 해당하는 소수성의 극성인 polysulfone과 결합시킨 불균질의 복합막을 사용하였다. 첫 번째 조사되지 않은 막에서 $OH^-$, $Cl^-$, $Na^+$의 초기플럭스는 실험조건을 제막의 두께 $80-200{\mu}m$로 할 때 정비례로 감소하였고, MeVP-DVBI의 농도를 20-80%로 할 때 ($OH^-$, $Cl^-$는 지수 값으로 증가하였고 $Na^+$는 지수 값으로 감소하여 음이온($OH^-$, $Cl^-$)의 능동전달을 증대시키고 NaOH의 농도를 0-0.5mol/L로 할 때 $0-2mole/cm^2{\cdot}h$로 지수 값으로 증가하였다. 두 번째 조사된 막에서 $OH^-$, $Cl^-$, $Na^+$의 초기플럭스는 대체적으로 조사되지 않은 막과 비교해서 감소하여 음이온의 능동전달이 감소하였는데 이는 방사선조사로 $H^+$이온 농도의 증가를 가져와 음이온의 초기플럭스가 감소하였기 때문으로 생각된다. 조사된 막의 pH의 추진력은 조사되지 않은 막보다 유의성 있게 증가하였고 세포막모델에서 $OH^-$와 $Cl^-$의 능동전달특성이 비정상적이기 때문에 세포장해가 세포에서 발현된다.

Keywords

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