Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Free Radical Scavenging Effect and Oxidative Stress Protective Activity of Domestic Processed Polygoni Multiflori Radix

국내산 법제 하수오의 라디칼 소거능 및 산화적 스트레스 개선 효과

  • Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Kim, Jun Young (Busan Regional Korea Food and Drug Administration, Ministry of Food and Drug Safety) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Myung (Department of Food Science and Nutrition, Pusan National University) ;
  • Hwang, Chung Eun (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Hee Yul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Ahn, Min Ju (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Jin Hwan (National Institute of Chemical Safety, Ministry of Environment) ;
  • Kim, Yun-Geun (Sancheong Oriental Medicinal Herb Institute) ;
  • Ko, Keon Hee (Sancheong Oriental Medicinal Herb Institute) ;
  • Goo, Young-Min (Sancheong Oriental Medicinal Herb Institute) ;
  • Oh, Kyeong Yeol (Sancheong Oriental Medicinal Herb Institute) ;
  • Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
  • 김현영 (경남과학기술대학교 식품과학과) ;
  • 김준영 (식품의약품안전처 부산지방식품의약품안전청) ;
  • 조은주 (부산대학교 식품영양학과) ;
  • 최지명 (부산대학교 식품영양학과) ;
  • 황정은 (경남과학기술대학교 식품과학과) ;
  • 이희율 (경남과학기술대학교 식품과학과) ;
  • 안민주 (경남과학기술대학교 식품과학과) ;
  • 이진환 (환경부 화학물질안전원) ;
  • 김윤근 ((재)산청한방약초연구소) ;
  • 고건희 ((재)산청한방약초연구소) ;
  • 구영민 ((재)산청한방약초연구소) ;
  • 오경렬 ((재)산청한방약초연구소) ;
  • 조계만 (경남과학기술대학교 식품과학과)
  • Received : 2015.04.08
  • Accepted : 2015.05.29
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we confirmed biological compounds from methanol (MeOH) extract of processed Polygoni Multiflori Radix (PPMR), and the radical scavenging effect and oxidative stress protective activity of MeOH extract of PPMR were investigated under in vitro conditions using LLC-$PK_1$ renal epithelial cells. In HPLC analysis, MeOH extract of PPMR contained four species of biological compounds named 2,3,5,4'-tetrahydroxystilbene 2-O-${\beta}$-D-glucoside, emodin, chrysophanol, and rhein. 2,3,5,4'-Tetrahydroxystilbene 2-O-${\beta}$-D-glucoside was detected as the main compound in PPMR as 115.02 mg/kg. MeOH extract of PPMR showed 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS), and hydroxyl radical scavenging activities in a concentration- dependent manner. In particular, upon $50{\mu}g/mL$ of PPMR extract treatment, DPPH, ABTS, and hydroxyl radical scavenging activities were approximately 48.4%, 57.9%, and 81.2%, respectively. LLC-$PK_1$ cell viability declined in response to oxidative stress induced by pyrogallol, sodium nitroprusside (SNP), and morpholinosydnonimine (SIN-1) generators of NO, $O_2{^-}$, and $ONOO^-$, respectively. However, MeOH extract of PPMR significantly and dose-dependently inhibited oxidative-stressed LLC-$PK_1$ cell cytotoxicity. In fact, upon $50{\mu}g/mL$ of PPMR extract treatment, LLC-$PK_1$ cell viabilities were approximately 82.1%, 89.1%, and 77.6% compared to stress levels induced by pyrogallol, SNP, and SIN-1, respectively.

본 연구에서는 국내산 법제 하수오 메탄올 추출물로부터 생리활성물질을 확인하였고, 추출물의 라디칼 소거능과 LLC-$PK_1$ cell을 이용한 산화적 스트레스 개선 효과를 살펴보았다. HPLC 분석 결과 4종의 생리활성물질인 2,3,5,4'-tetrahydroxystilbene 2-O-${\beta}$-D-glucoside, emodin, chrysophanol 및 rhein을 확인하였다. 특히 주요 화합물인 2,3, 5,4'-tetrahydroxystilbene 2-O-${\beta}$-D-glucoside는 115.02 mg/kg이었다. 법제 하수오 추출물의 DPPH, ABTS, hydroxyl 라디칼 소거능은 농도 의존적으로 증가하였다. 추출물의 $50{\mu}g/mL$ 처리 시 DPPH 라디칼 소거능은 48.4%, ABTS 라디칼 소거능은 57.9% 및 hydroxyl 라디칼 소거능은 81.2%로 나타내었다. 한편 LLC-$PK_1$ cell에서 각각의 NO, $O_2{^-}$, 및 $ONOO^-$ 생성물질인 pyrogallol, sodium nitroprusside(SNP) 및 morpholinosydnonimine(SIN-1) 처리에 의해 유도된 산화적 스트레스 상에서 세포 생존율은 감소하였다. 그러나 법제 하수오 메탄올 추출물은 농도 의존적으로 세포 독성을 저해하였다. 추출물의 $50{\mu}g/mL$ 처리 시 세포 생존율은 각각 82.1%(pyrogallol), 89.1%(SNP) 및 77.6%(SIN-1)였다.

Keywords

Acknowledgement

Supported by : 농림축산식품부

References

  1. Kim IC. 2008. Antioxidative properties and whitening effects of the Polygoni Multiflori Radix, Polygonati Rhizoma and Ephedrae Herba. J Korean Oil Chemists' Soc 25: 533-538.
  2. Sin SM, Mok SY, Lee S, Cho KM, Cho EJ, Kim HY. 2011. Protective effect of bitter melon (Momordica charantia) against oxidative stress. Cancer Prev Res 16: 86-92.
  3. Oberley TD, Schultz JL, Li N, Oberley LW. 1995. Antioxidant enzyme levels as a function of growth state in cell culture. Free Radic Biol Med 19: 53-65. https://doi.org/10.1016/0891-5849(95)00012-M
  4. Darley-Usmar V, Wiseman H, Halliwell B. 1995. Nitric oxide and oxygen radicals: a question of balance. FEBS Lett 369: 131-135. https://doi.org/10.1016/0014-5793(95)00764-Z
  5. Lim DG. 2004. Oxidative stress: reactive oxygen species and nitric oxide. Korean J Crit Care Med 19: 81-85.
  6. Kim HY, Lee YA, Cho EJ. 2012. Free radical scavenging effect and protective activity from oxidative stress of broccoli and sprouts. CNU J Agric Sci 39: 81-86. https://doi.org/10.7744/cnujas.2012.39.1.081
  7. Choi JM, Choi MJ, Lee SL, Yamabe N, Lee S, Cho EJ. 2012. Protective effects of ethylacetate fraction from Taraxacum coreanum against peroxynitrite-induced oxidative damage under cellular system. Cancer Prev Res 17: 251-256.
  8. Andersen HR, Nielsen JB, Nielsen F, Grandjean P. 1997. Antioxidative enzyme activities in human erythrocytes. Clin Chem 43: 562-568.
  9. Nuttall SL, Kendall MJ, Martin U. 1999. Antioxidant therapy for the prevention of cardiovascular disease. OJM 92: 239-244.
  10. Kim HY, Hwang BR, Wu TT, Cho EJ. 2012. The protective effect of Perilla frutescens from ONOO--induced oxidative stress and antiaging effect under cellular system. CNU J Agric Sci 39: 467-471. https://doi.org/10.7744/cnujas.2012.39.4.467
  11. Kim HY, Sin SM, Lee S, Cho KM, Cho EJ. 2013. The butanol fraction of bitter melon (Momordica charantia) scavenges free radicals and attenuates oxidative stress. Prev Nutr Food Sci 18: 18-22. https://doi.org/10.3746/pnf.2013.18.1.018
  12. Bae SH, Kim DH, Bae YS, Lee KJ, Kim DW, Yoon JB, Hong JH, Kim SH. 2010. Toxic hepatitis associated with Polygoni multiflori. Korean J Hepatol 16: 182-186. https://doi.org/10.3350/kjhep.2010.16.2.182
  13. Choi J, Lee H, Kim Y, Kim B, Kim I, Lee C. 2012. Effect of Polygonum multiflorum Thunberg extract on lipid metabolism in rats fed high-cholesterol diet. J Korean Soc Food Sci Nutr 41: 957-962. https://doi.org/10.3746/jkfn.2012.41.7.957
  14. Chiu PY, Mak DH, Poon MK, Ko KM. 2002. In vivo antioxidant action of a lignan-enriched extract of Schisandra fruit and an anthraquinone-containing extract of Polygonum root in comparison with schisandrin B and emodin. Planta Med 68: 951-956. https://doi.org/10.1055/s-2002-35661
  15. Yim TK, Wu WK, Mak DH, Ko KM. 1998. Myocardial protective effect of an anthraquinone-containing extract of Polygonum multiflorum ex vivo. Planta Med 64: 607-611. https://doi.org/10.1055/s-2006-957531
  16. Wang W, Cao CY, Wang DQ, Zhao DZ. 2006. Effect of prepared Polygonum multiflorum on striatum extracellular acetylcholine and choline in rats of intracerebral perfusion with sodium azide. Zhongguo Zhong Yao Za Zhi 31: 751-753.
  17. Xiao PG, Xing ST, Wang LW. 1993. Immunological aspects of Chinese medicinal plants as antiageing drugs. J Ethnopharmacol 38: 167-175. https://doi.org/10.1016/0378-8741(93)90013-U
  18. Liang Z, Chen H, Yu Z, Zhao Z. 2010. Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry. Chin Med 5: 29. https://doi.org/10.1186/1749-8546-5-29
  19. Choi JS, Kim HY, Seo WT, Lee JH, Cho KM. 2012. Roasting enhances antioxidant effect of bitter melon (Momordica charantia L.) increasing in flavan-3-ol and phenolic acid contents. Food Sci Biotechnol 21: 19-26. https://doi.org/10.1007/s10068-012-0003-7
  20. Zhang YZ, Shen JF, Xu JY, Xiao JH, Wang JL. 2007. Inhibitory effects of 2,3,5,4'-tetrahydroxystilbene-2-O-${\beta}$-D-glucoside on experimental inflammation and cyclooxygenase 2 activity. J Asian Nat Prod Res 9: 355-363. https://doi.org/10.1080/10286020600727772
  21. Wang X, Zhao L, Han T, Chen S, Wang J. 2008. Protective effects of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside, an active component of Polygonum multiflorum Thunb, on experimental colitis in mice. Eur J Pharmacol 578: 339-348 https://doi.org/10.1016/j.ejphar.2007.09.013
  22. Zeng C, Xiao JH, Chang MJ, Wang JL. 2011. Beneficial effects of THSG on acetic acid-induced experimental colitis: Involvement of upregulation of PPAR-$\gamma$ and inhibition of the Nf-Κb inflammatory pathway. Molecules 16: 8552-8568. https://doi.org/10.3390/molecules16108552
  23. Lv LS, Gu XH, Tang J, Ho CT, Tang J. 2006. Stilbene glycosides from the roots of Polygonum multiflorum Thunb and their in vitro antioxidant activities. J Food Lipids 13: 131-144. https://doi.org/10.1111/j.1745-4522.2006.00039.x
  24. Lv LS, Gu XH, Tang J, Ho CT. 2007. Antioxidant activity of stilbene glycoside from Polygonum multiflorum Thunb in vivo. Food Chem 104: 1678-1681. https://doi.org/10.1016/j.foodchem.2007.03.022
  25. Liu QL, Xiao JH, Ma R, Ban Y, Wang JL. 2007. Effect of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside on lipoprotein oxidation and proliferation of coronary arterial smooth cells. J Asian Nat Prod Res 9: 689-697. https://doi.org/10.1080/17415990500209064
  26. Chiang YC, Huang GH, Ho YL, Hsieh PC, Chung HP, Chou FI, Chang YS. 2011. Influence of gamma irradiation on microbial load and antioxidative characteristics of Polygoni Multiflori Radix. Process Biochem 46: 777-782. https://doi.org/10.1016/j.procbio.2010.12.004
  27. Um MY, Choi WH, Aan JY, Kim SR, Ha TY. 2006. Protective effect of Polygonum multiflorum Thunb on amyloid ${\beta}$- peptide 25-35 induced cognitive deficits in mice. J Ethnopharmacol 104: 144-148. https://doi.org/10.1016/j.jep.2005.08.054
  28. Wang R, Tang Y, Feng B, Ye C, Fang L, Zhang L, Li L. 2007. Changes in hippocampal synapses and learning-memory abilities in age-increasing rats and effects of tetrahydroxystilbene glucoside in aged rats. Neuroscience 149: 739-746. https://doi.org/10.1016/j.neuroscience.2007.07.065
  29. Chen Y, Wang M, Rosen RT, Ho CT. 1999. 2,2-Diphenyl-1- picrylhydrazyl radical-scavenging active components from Polygonum multiflorum Thunb. J Agric Food Chem 47: 2226- 2228. https://doi.org/10.1021/jf990092f
  30. Luo A, Fan Y, Luo A. 2011. In vitro free radicals scavenging activities of polysaccharide from Polygonum multiflorum Thunb. J Med Plants Res 5: 966-972.
  31. Guan S, Su W, Wang N, Li P, Wang Y. 2008. A potent tyrosinase activator from Radix Polygoni multiflori and its melanogenesis stimulatory effect in B16 melanoma cells. Phytother Res 22: 660-663. https://doi.org/10.1002/ptr.2358
  32. Jiang ZQ, Xu JM, Long MH, Tu ZM, Yang GX, He GY. 2009. 2,3,5,4'-tetrahydroxystilbene-2-O-${\beta}$-D-glucoside (THSG) induces melanogenesis in B16 cells by MAP kinase activation and tyrosinase upregulation. Life Sci 85: 345-350. https://doi.org/10.1016/j.lfs.2009.05.022
  33. Park HJ, Zhang N, Park DK. 2011. Topical application of Polygonum multiflorum extract induces hair growth of resting hair follicles through upregulating Shh and ${\beta}$-catenin expression in C57BL/6 mice. J Ethnopharmacol 135: 369-375. https://doi.org/10.1016/j.jep.2011.03.028
  34. Yen GC, Chen HW, Duh PD. 1998. Extraction and identification of an antioxidative component from Jue Ming Zi (Cassia tora L.). J Agric Food Chem 46: 820-824. https://doi.org/10.1021/jf970690z
  35. Chang CH, Lin CC, Yang JJ, Namba T, Hattori M. 1996. Anti-inflammatory effects of emodin from Ventilago leiocarpa. Am J Chin Med 24: 139-142. https://doi.org/10.1142/S0192415X96000189
  36. Hatano T, Uebayashi H, Ito H, Shiota S, Tsuchiya T, Yoshida T. 1999. Phenolic constituents of cassia seeds and antibacterial effect of some naphthalenes and anthraquinones on methicillin-resistant Staphylococcus aureus. Chem Pharm Bull (Tokyo) 47: 1121-1127. https://doi.org/10.1248/cpb.47.1121
  37. Kuo YC, Sun CM, Ou JC, Tsai WJ. 1997. A tumor cell growth inhibitor from Polygonum hypoleucum Ohwi. Life Sci 61: 2335-2344. https://doi.org/10.1016/S0024-3205(97)00937-5
  38. Chan TC, Chang CJ, Koonchanok NM, Geahlen RL. 1993. Selective inhibition of the growth of ras-transformed human bronchial epithelial cells by emodin, a protein-tyrosine kinase inhibitor. Biochem Biophys Res Commun 193: 1152-1158. https://doi.org/10.1006/bbrc.1993.1746
  39. Shao L, Zhao SJ, Cui TB, Liu ZY, Zhao W. 2012. 2,3,5,4'-tetrahydroxystilbene-2-O-${\beta}$-D-glycoside biosynthesis by suspension cells cultures of Polygonum multiflorum Thunb and production enhancement by methyl jasmonate and salicylic acid. Molecules 17: 2240-2247. https://doi.org/10.3390/molecules17022240
  40. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  41. Hatano T, Edamatsu R, Hiramatsu M, Mori A, Fujita Y, Yasuhara T, Yoshida T, Okuda T. 1989. Effects of the interaction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull 37: 2016-2021. https://doi.org/10.1248/cpb.37.2016
  42. Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. 2002. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 13: 8-17. https://doi.org/10.1002/pca.611
  43. Lee YA, Kim HY, Cho EJ. 2005. Comparison of methanol extracts from vegetables on antioxidant effect under in vitro and cell system. J Korean Soc Food Sci Nutr 34: 1151-1156. https://doi.org/10.3746/jkfn.2005.34.8.1151
  44. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  45. Okuda M, Tsuda K, Masaki K, Hashimoto Y, Inui K. 1999. Cisplatin-induced toxicity in LLC-PK1 kidney epithelial cells: role of basolateral membrane transport. Toxicol Lett 106: 229-235. https://doi.org/10.1016/S0378-4274(99)00071-5
  46. Yokozawa T, Satoh A, Cho EJ, Kashiwada Y, Ikeshiro Y. 2005. Protective role of Coptidis Rhizoma alkaloids against peroxynitrite-induced damage to renal tubular epithelial cells. J Pharm Pbarmacol 57: 367-374. https://doi.org/10.1211/0022357055470
  47. Cui FJ, Li TZ, Lee SJ, Park SJ, Lim Y, Kim KA, Chang BJ, Lee JH, Lee MH, Choe NH. 2006. The effects of airborne particulate matters on the alveolar macrophages for the iNOS expression and nitric oxide with nitrotyrosilatedproteins formation. Tuberc Respir Dis 60: 426-436. https://doi.org/10.4046/trd.2006.60.4.426
  48. Radi R, Beckman JS, Bush KM, Freeman BA. 1991. Peroxynitrite- induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch Biochem Biophys 288: 481-487. https://doi.org/10.1016/0003-9861(91)90224-7

Cited by

  1. Effect of roasting time on physicochemical characteristics and antioxidant activity of hot water extracts of dried Liriope platyphylla vol.25, pp.2, 2018, https://doi.org/10.11002/kjfp.2018.25.2.246
  2. Perception of Use of Environment-friendly Agricultural Products during School Foodservice of Mothers of Elementary School Students in Gyeonggi vol.23, pp.3, 2018, https://doi.org/10.5720/kjcn.2018.23.3.234
  3. Annual Analysis on Quality Attributes and Customer Satisfaction in School Foodservice vol.42, pp.8, 2009, https://doi.org/10.4163/kjn.2009.42.8.770
  4. Effects of Cynanchi Wilfordii Radix and Polygoni Multiflori Radix liquors on lipid peroxidation and antioxidant activity in rat serum amd brain tissue vol.26, pp.3, 2015, https://doi.org/10.11002/kjfp.2019.26.3.350
  5. Comparison of Active Compounds for Each Supplement after Processing Methods of KIOM Polygonum multiflorum Thunberg Using Various Ingredient(s) vol.54, pp.5, 2015, https://doi.org/10.14397/jals.2020.54.5.37
  6. Protective Effect of Processed Polygoni multiflori Radix and Its Major Substance during Scopolamine-Induced Cognitive Dysfunction vol.9, pp.2, 2015, https://doi.org/10.3390/pr9020342