Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Optimization of Fermentation Medium for Glycyrrhizin Biotransformation to Monoglucuronyl-glycyrrhetinic Acid by Plackett-Burman and Box-Behnken Design

  • Quan, Yanling (Department of Chemistry and Engineering, University of Science and Technology of Liaoning) ;
  • Wang, Lin (Department of Chemistry and Engineering, University of Science and Technology of Liaoning) ;
  • Liu, Yisheng (Department of Chemistry and Engineering, University of Science and Technology of Liaoning) ;
  • Cong, Jingxiang (Department of Chemistry and Engineering, University of Science and Technology of Liaoning) ;
  • Xie, Shengquan (Liaoning Economic Vocational Technology Institute) ;
  • Wu, Xiuhong (Department of Chemistry and Engineering, University of Science and Technology of Liaoning)
  • Received : 2014.07.31
  • Accepted : 2014.10.13
  • Published : 2015.06.01
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Abstract

Plackett-Burman (PB) and Box-Behnken (BB) experimental designs were used to optimize fermentation variables for the biotransformation of glycyrrhizin (GL) to monoglucuronyl-glycyrrhetinic acid (MGGA). The PB design was first used to screen the important factors among the medium variables. The steepest ascent method was used to approach the optimum range for each of these factors. The BB design was finally used to analyze the response surfaces of the screened factors for further optimization. The optimized conditions for this system were 0.7 g/L $MgSO_4{\cdot}7H_2O$, 1.19 g/L GL, and cultivation for six days. The biotransformation of GL to MGGA could reach up to 35.72%, which is a good result for this kind of transformation.

Keywords

References

  1. Yun, T. K. and Choi, S. Y., "Preventive Effect of Ginseng Intake Against Various Human Cancers: A Case-Control Study on 1987 Pairs," Cancer. Epidem. Biomar., 4(4), 401-408(1995).
  2. Rudakewich, M., Ba, F. and Benishin, C. G., "Neurotrophic and Neuroprotective Actions of Ginsenosides Rb1and Rg1," Planta. Med., 67, 533-537(2001). https://doi.org/10.1055/s-2001-16488
  3. Peng, L., Sun, S., Xie, L. H., Wicks, S. M. and Xie, J. T., "Ginsenoside Re: Pharmacological Effects on Cardiovascular System," Cardiovasc. Ther., 30(4), 183-188(2002).
  4. Wang, Z. X., Song, J. L. and Yang, G. Z., "Current status of Study on Glycyrrhizin," Chinese. J. Integr. Tradit. West. Med., 22, 796-798(2002).
  5. Cong, J. X. and Lin, B. C., "Separation of Liquiritin by Simulated Moving Bed Chromatography," J. Chromatogr. A., 1145, 190-194 (2007). https://doi.org/10.1016/j.chroma.2007.01.088
  6. Wang, X. and Zhang, H. I., "Advances in Studies on Pharmacological Activities of Ginkgolides," Chinese. Tradit. Herbal. Drugs. 36(11), 1741-1744(2005).
  7. Xia, S. H. and Fang, D. C., "Pharmacological Action and Mechanisms of Ginkgolides B," Chinese. Med. J., 120(10), 922-928 (2007).
  8. Clarke, K., Lee, F. T., Brechbiel, M. W., Smyth, F. E., Old, L. J., and Scott, A. M., "Therapeutic Efficacy of Anti-Lewisy Humanized 3S193 Radioimmunotherapy in a Breast Cancer Model: Enhanced Activity When Combined with Taxol Chemotherapy," Clin. Cancer. Res., 6, 3621-3628(2000).
  9. Hou, J. G., Xue, J. J., Wang, C. Y., Liu, L., Zhang, D. L., Wang, Z., Li, W., Zheng, Y. N. and Sung, C. K., "Microbial Transformation of Ginsenoside Rg3 to Ginsenoside Rh2 by Esteya Vermicola CNU 120806," World. J. Microbiol. Biotechnol., 28, 1807-1811(2012). https://doi.org/10.1007/s11274-011-0946-5
  10. Kim, H. S., Lee, E. H., Ko, S. R., Choi, K. J., Park, J. H. and Im, D. S., "Effects of Ginsenosides Rg3 and Rh2 on the Proliferation of Prostate Cancer Cells," Arch. Pharrn.Res. 27(4), 429-435(2004). https://doi.org/10.1007/BF02980085
  11. Sirikantaramas, S., Asano, T., Sudo, H., Yamazaki, M. and Saito, K., "Camptothecin: Therapeutic Potential and Biotechnology," Curr. Pharm. Biotechno., 8, 196-202(2007). https://doi.org/10.2174/138920107781387447
  12. Liu, Y. C., Chen, G. Y., Ge, F. L., Li, W., Zeng, L. H. and Cao, W. G., "Efficient Biotransformation of Cholesterol to Androsta- 1,4-diene-3,17-dione by a Newly Isolated Actinomycete Gordonia Neofelifaecis," World. J. Microbiol. Biotechnol. 27, 759-765 (2011). https://doi.org/10.1007/s11274-010-0513-5
  13. Gouiric, S. C., Feresin, G. E., Tapia, A. A., Rossomando, P. C., Schmeda-Hirschmann, G. and Bustos, D. A., "7b-Dihydroxydehydroabietic acid, a New Biotransformation Product of Dehydroabietic Acid by Aspergillus Niger," World. J. Microbiol. Biotechnol. 20, 281-284(2004). https://doi.org/10.1023/B:WIBI.0000023834.60165.79
  14. Hu, S. C., Hong, K., Song, Y. C., Liu, J. Y. Tan, R. X., "Biotransformation of Soybean Isoflavones by a Marine Streptomyces sp. 060524 and Cytotoxicity of the Products," World. J. Microbiol. Biotechnol., 25, 115-121(2009). https://doi.org/10.1007/s11274-008-9872-6
  15. Zou, S. P., Zhou, J. J., Kaleem, I., Xie, L. P., Liu, G. Y., Li, C., "Preparative Enrichment and Separation of Glycyrrhetinic Acid Monoglucuronide from Fermentation Broths with Macroporous Resins," Sep. Sci Technol., 47, 1055-1062(2012). https://doi.org/10.1080/01496395.2011.637280
  16. Li, Y., Liu, Z. Q., Cui, F. J., Liu, Z. S. and Zhao, H., "Application of Plackett-Burman Experimental Design and Doehlert Design to Evaluate Nutritional Requirements for Xylanase Production by Alter Naria Mali ND-16," Appl. Microbiol. Biotechnol. 77, 285-291(2007). https://doi.org/10.1007/s00253-007-1167-6
  17. Palvannan, T. and Sathishkumar, P., "Production of Laccase from Pleurotus florida NCIM 1243using Plackett-Burman Design and Response Surface Methodology," J. Basic. Microb. 50, 325-335 (2010). https://doi.org/10.1002/jobm.200900333
  18. Plackett, R. L. and Burman, J. P., "The Design of Optimum Multifactorial Experiments," Biometrika, 37, 305-325(1946).
  19. Jacques, P., Hbid, C., Destain, J., Razafindralambo, H., Paquot, M., Pauw, E. D. and Thonart, P., "Optimization of Biosurfactant Lipopeptide Production from Bacillus subtilis S499 by Plackett-Burman Design," Appl. Biochem. Biotechnol., 77-79, 223-233 (1999).
  20. Yue, P. F., Zheng, Q., Wu, B., Hu, P. Y., Wu, Z. F. and Yang, M., "Application of Plackett-Burman Design and Box-Behnken Design to Achieve Process Optimization for Geniposide Submicron Emulsion," J. Dispersion Sci. Technol., 33, 213-222(2012). https://doi.org/10.1080/01932691.2011.561162
  21. Mahdavi, V. and Monajem, A., "Statistical Optimization for Oxidation of Ethyl Benzene over Co- Mn/SBA-15 catalyst by Box-Behnken Design," Korean J. Chem. Eng., 33, 2178-2185(2013).
  22. Wu, S. J., Yong, Z. J., Zhu, L. H. and Jin, F. X., "Study on Biotransformation of Glycyrrhizin," Chinese. Tradit. Herbal. Drugs. 34, 516-518(2003).

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