DOI QR코드

DOI QR Code

족삼리의 선모(仙茅)약침이 Monosodium Iodoacetate로 유발한 골관절염 흰쥐에 미치는 영향

Effects of Curculiginis Rhizoma Pharmaco-Acupuncture at $ST_{36}$ on Monosodium Iodoacetate(MIA)-induced Osteoarthritic Rats

  • 지민정 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 김재수 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이현종 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구경혈학교실)
  • Ji, Min Jung (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Deagu Haany University) ;
  • Lim, Seong Chul (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Deagu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Deagu Haany University) ;
  • Lee, Hyun Jong (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Deagu Haany University) ;
  • Lee, Yun Kyu (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Deagu Haany University)
  • 투고 : 2015.02.09
  • 심사 : 2015.02.23
  • 발행 : 2015.03.20

초록

Objectives : This study was designed to investigate effects of Curculiginis Rhizoma pharmaco-acupuncture at $ST_{36}$ on monosodium iodoacetate-induced osteoarthritic rats. Methods : Twenty rats were divided into four groups consisting of 5 rats: rats receiving no injection(normal), rats injected with monosodium Iodoacetate(MIA, control), rats injected with MIA and normal saline(N-S), and rats injected with MIA and Curculiginis Rhizoma (CRPA). N-S and CRPA were administered once a day at $ST_{36}$ during 21 days. After that we examined the weight-bearing ability of hind paws, liver and kidney function, immunocell, cytokines, proteins, and gene expression of cytokines. Injury of synovial tissue was measured by H & E, Safranin O immunofluorescence. Results : The weight-bearing ability of the hind paws, Serum TNF-${\alpha}$, IL-$1{\beta}$, IL-6, PGE2, LTB4, DPD, Osteocalcin, Protein COX-2 of CRPA decreased significantly. Protein Arachidonate 5 lipoxygenase of CRPA was decreased, but not significantly. Expression of gene COX-2, TNF-${\alpha}$, IL-$1{\beta}$, IL-6, NOS2 of CRPA decreased. In histological observations, CRPA was improved, compared with other control groups. Conclusions : It can be suggested that Curculiginis Rhizoma pharmaco-acupuncture at $ST_{36}$ has anti-inflammatory and pain relief effects on monosodium iodoacetate-induced osteoarthritic rats.

키워드

참고문헌

  1. Korean Acupuncture & Moxibustion Medicine Society Textbook Compilation Committe. Acupuncture & Moxibustion Medicine. Gyeonggi : Jipmoondang. 2012 : 230, 549-64, 577-84.
  2. The Korean Orthopaedic Association. Orthopedixs. The 7th Edition. Seoul : Newest Medicine Company. 2013 : 49-50, 99-100, 317-26, 1012-4.
  3. Kang MK, Nam SS, Lee YH. The study on acupuncture for osteoarthritis. The Acupuncture. 2003 ; 20(2) : 18-28.
  4. Korean Pharmacopuncture Institute, Pharmacopuncture Medical Mnstitute. Pharmacopuncturology. Seoul : Elsevierkorea. 2011 : 3-8.
  5. Kim WY, Choi JB. Effects of bee venom and cervi cornu parvum pharmacoacupuncture in monosodium iodoacetate(MIA)-induced osteoarthritis rat. JKMR. 2010 ; 20(1) : 61-77.
  6. Lee OJ, Lee DG, Lee JH et al. Effects of Curcuma Longa linne pharmacopuncture at $ST_{36}$ on adjuvant induced arthritis in rats. The Acupuncture. 2013 ; 30(4) : 95-105. https://doi.org/10.13045/acupunct.2013026
  7. Koo MS, Yoon JH, Kim KH, Jang JH, Lee SD, Kim KS. The effect of Bupleuri Radix herbal-acupuncture solution on immune responses to adjuvant induced arthritis in mice. The Acupuncture. 2002 ; 19(3) : 51-63.
  8. Bae CW, Kim KH, Hwang HS, Kim YS. Effect of Strychni Semen aqua-acupuncture in rats with arthritis induced by freund's complete adjuvant. The Acupuncture. 2001 ; 18(1) : 217-25.
  9. Korean Medicine Teaching Meterial Editing Committee. Herbology. Seoul : Youngrimsa. 2011 : 601
  10. Ku SK, Kim JS, Seo YB et al. Effect of Curculigo Orchioides on reflux esophagitis by suppressing proinflammatory cytokines. The American Journal of Chinese Medicine. 2012 ; 4(6) : 1241-55.
  11. Roh SS. Effects of Curculiginis Rhizoma on antithrombotic activity. Korean Journal Herbology. 2011 ; 26(4) : 124-32.
  12. Wang ZQ, Li JL, Sun YL et al. Chinese herbal medicine for osteoporosis: a systematic review of randomized controlled trails. E-CAM. 2013. Available from : http://dx.doi.org/10.1155/2013/356260.
  13. An JU, Yu GY. Statistical analysis of biomedical data. Seoul : Hannarae. 2007 : 312-78.
  14. Kim HR. Prevalence and risk factors of osteoarthritis among women and men elderly people in Korea. Korea Gerontological Society. 2013 ; 33(4) : 805-20.
  15. Yoon CH. Current clinical practice: osteoarthritis update. Korean Journal of Medicine. 2012 ; 82(2) : 170-4. https://doi.org/10.3904/kjm.2012.82.2.170
  16. Kim MJ, Yoon JH, Kim KH, Lee SD, Kim KS. Acupuncture therapy literature study on the knee rheumatoid arthritis. The Acupuncture. 2005 ; 22(1) : 191-201.
  17. Janusz MJ, Hookfin EB, Heitmeyer SA et al. Moderation of iodoacetate-induced experimental osteoarthritis in rats by matrix metalloproteinase inhibitors. Osteoarthritis Cartilage. 2001 ; 9(8) : 751-60. https://doi.org/10.1053/joca.2001.0472
  18. Wang XD, Kou XX, He DQ et al. Progression of cartilage degradation, bone resorption and pain in rat temporomandibular joint osteoarthritis induced by injection of iodoacetate. PLoS One. 2012 ; 7(9) : e45036. Published online Sep 11, 2012. Available from : http://www.ncbi.nlm.nih.gov/ pmc/articles/PMC3439407/pdf/pone.0045036.pdf. https://doi.org/10.1371/journal.pone.0045036
  19. Neugebauer V, Han JS, Adwanikar H, Fu Y, Ji G. Techniques for assessing knee joint pain in arthritis. Molecular Pain. 2007 ; 3 : 8. Available from : http://www.molecularpain.com/content/3/ 1/8. https://doi.org/10.1186/1744-8069-3-8
  20. Lee HJ. Introduction and clinic of pharmacoacupuncture. Seoul : Iljungsa. 1999 : 16-8.
  21. Baik SI, Ahn JC, Kim YJ, Kim HS, Kwon KR. Type analysis of pharmacopuncture papers published in the journal of Korean acupuncture and moxibustion. The Acupuncture. 2006 ; 23(6) : 19-27.
  22. An YG. Gyuonghulhakchongseo. Seoul : Seongbosa. 2002 : 196-7.
  23. Kim JC, Lee EY, Seo IB, Koo ST, Choi SM, Kim EW. Effects of low frequency electro-acupuncuture( ST36) on the adjuvant-induced arthritis in rats. The Acupuncture. 2006 ; 23(6) : 207-19.
  24. Lee YS. Behavioral and histological changes of monosodium iodoacetate(MIA) induced osteoarthritis in rats. Seoul : Korea Univ. 2008. Korean.
  25. Stannus O, Jones G, Cicuttini F et al. Circulating levels of IL-6 and TNF-${\alpha}$ are associated with knee radiographic osteoarthritis and knee cartilage loss in older adults. Osteoarthritis and Cartilage. 2010 ; 18(11) : 1441-7. https://doi.org/10.1016/j.joca.2010.08.016
  26. Wong PK, Quinn JM, Sims NA, van Nieuwenhuijze A, Campbell IK, Wicks IP. Interleukin-6 modulates production of T lymphocyte-derived cytokines in antigen-induced arthritis and drives inflammation-induced osteoclastogenesis. Arthritis & Rheumatism. 2006 ; 54(1) : 158-68. https://doi.org/10.1002/art.21537
  27. Haseeb A, Haqqi TM. Immunopathogenesis of osteoarthritis. clinical immunology. 2013 ; 146(3) : 185–96. https://doi.org/10.1016/j.clim.2012.12.011
  28. Chen SH, Fahmi H, Shi Q, Benderdour M. Regulation of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase-activating protein/5-lipoxygenase by 4-hydroxynonenal in human osteoarthritic chondrocytes. Arthritis Research & Therapy. 2010 ; 12 : R21. Available from : http://arthr itis-research.com/content/12/1/R21. https://doi.org/10.1186/ar2926
  29. Park JH. Pathophysiology of articular cartilage injury. J Korean Orthop Sports Med. 2005 ; 4(1) : 6-11.
  30. Caroline Belanger, Hanan Elimam, Julie Lefebvre, Pierre Borgeat, Sylvie Marleaul. Involvement of endogenous leukotriene B4 and plateletactivating factor in polymorphonuclear leucocyte recruitment to dermal inflammatory sites in rats. Immunology. 2008 ; 124(3) : 295-303. https://doi.org/10.1111/j.1365-2567.2007.02767.x
  31. M Lotz, J Martel-Pelletier, C Christiansen et al. Value of biomarkers in osteoarthritis: current status and perspectives. Annals of the Rheumatic Diseases. 2013 ; 72(11) : 1756-63. https://doi.org/10.1136/annrheumdis-2013-203726
  32. M Takahashi, K Kushida, H Hoshino et al. Concentrations of pyridinoline and deoxypyridinoline in joint tissues from patients with osteoarthritis or rheumatoid arthritis. Annals of the Rheumatic Diseases. 1996 ; 55(5) : 324-37. https://doi.org/10.1136/ard.55.5.324
  33. Lian JB, Gundberg CM. Osteocalcin. Biochemical considerations and clinical applications. Clinical Orthopaedics and Related Research. 1988 ; 226(1) : 267-91.
  34. A Fairney, K V Patel, N P Hollings, MH Seifert. Abnormal osteocalcin binding in rheumatoid arthritis. Annals of the Rheumatic Diseases. 1990 ; 49(4) : 229-30. https://doi.org/10.1136/ard.49.4.229
  35. Mary KC, Shawn OF. Biochemistry. The fifth edition. Seoul : Life Science Publishing Company. 2007 : 451.

피인용 문헌

  1. 선모(仙茅) 열수(熱水) 추출물의 Collagen 유발 관절염에 대한 약리 효능 연구 vol.31, pp.4, 2016, https://doi.org/10.6116/kjh.2016.31.4.1.
  2. 강황(薑黃) 약침이 Monosodium Iodoacetate 유도 골관절염 흰쥐에 미치는 영향 vol.29, pp.2, 2015, https://doi.org/10.18325/jkmr.2019.29.2.115