Journal of Acupuncture Research

  • 제32권1호
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  • Pages.1-11
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  • 2015
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
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카드뮴으로 유발된 산화적 스트레스에 대한 침 자극의 신장 보호 효과

The Protective Effects of Acupuncture on Oxidative Stress Caused by Cadmium in the Kidney

  • 신화영 (대구한의대학교 부속대구한방병원 침구의학과) ;
  • 이현종 (대구한의대학교 부속대구한방병원 침구의학과) ;
  • 김재수 (대구한의대학교 부속대구한방병원 침구의학과)
  • Shin, Hwa Young (Department of Acupuncture & Moxibustion Medicine, Daegu Oriental Hospital of Daegu Haany University) ;
  • Lee, Hyun Jong (Department of Acupuncture & Moxibustion Medicine, Daegu Oriental Hospital of Daegu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture & Moxibustion Medicine, Daegu Oriental Hospital of Daegu Haany University)
  • 투고 : 2014.12.03
  • 심사 : 2015.03.02
  • 발행 : 2015.03.20
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초록

Objectives : This study was performed to inquire into the protective effects of acupuncture on oxidative stress caused by cadmium accumulation in the kidney. Methods : Sprague-Dawley male($150{\pm}30g$) rats were stabilized for 1 week and divided into 5 groups: normal, control, $LR_3$ acupuncture, $BL_{23}$ acupuncture and sham acupuncture. For three days experimental groups received oral doses of cadmium 2 mg/kg twice a day. Acupuncture was applied bilaterally at each point 10 times for two weeks. The depth of stimulation was 1 mm at right angles and torsion of acupuncture was produced 2 times per second for 1 minute. The kidneys were extracted and weighed after two weeks, and renal function was confirmed through blood urea nitrogen(BUN). We measured reactive oxygen species of the serum and kidney, and compared expression levels of superoxide dismutase(SOD), catalase, glutathione peroxidase(Gpx), nuclear factor erythroid derived 2-related factor 2(Nrf-2), heme oxygenase-1(HO-1), nuclear factor-${\kappa}B$(NF-${\kappa}B)$, cyclooxygenase-2(COX-2), inducible nitric oxide synthase (iNOS), Bax and Cytochrome c. Results : The $LR_3$ acupuncture group and $BL_{23}$ acupuncture group experienced significantly increased kidney weight, and decreased BUN compared to control group. In terms of oxidative stress, the $LR_3$ acupuncture group and $BL_{23}$ acupuncture group experienced significantly reduced reactive oxygen species compared to the control group. Conclusions : The $LR_3$ acupuncture group and $BL_{23}$ acupuncture group experienced showed the effects of antioxidant, anti-inflammatory and apoptosis protection. The $BL_{23}$ acupuncture group was more effective than $LR_3$ acupuncture group.

키워드

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