DOI QR코드

DOI QR Code

Effect of Standardized Boesenbergia pandurata Extract and Its Active Compound Panduratin A on Skin Hydration and Barrier Function in Human Epidermal Keratinocytes

  • Woo, Seon Wook (Department of Biotechnology, Yonsei University) ;
  • Rhim, Dong-Bin (Department of Biomaterials Science and Engineering, Yonsei University) ;
  • Kim, Changhee (Department of Biotechnology, Yonsei University) ;
  • Hwang, Jae-Kwan (Department of Biotechnology, Yonsei University)
  • 투고 : 2014.12.15
  • 심사 : 2015.01.12
  • 발행 : 2015.03.31

초록

The skin plays a key role in protecting the body from the environment and from water loss. Cornified envelope (CE) and natural moisturizing factor (NMF) are considered as the primary regulators of skin hydration and barrier function. The CE prevents loss of water from the body and is formed by cross-linking of several proteins. Among these proteins, filaggrin is an important protein because NMF is produced by the degradation of filaggrin. Proteases, including matriptase and prostasin, stimulate the generation of filaggrin from profilaggrin and caspase-14 plays a role in the degradation of filaggrin. This study elucidated the effects of an ethanol extract of Boesenbergia pandurata (Roxb.) Schltr., known as fingerroot, and its active compound panduratin A on CE formation and filaggrin processing in HaCaT, human epidermal keratinocytes. B. pandurata extract (BPE) and panduratin A significantly stimulated not only CE formation but also the expression of CE proteins, such as loricrin, involucrin, and transglutaminase, which were associated with $PPAR{\alpha}$ expression. The mRNA and protein levels of filaggrin and filaggrin-related enzymes, such as matriptase, prostasin, and caspase-14 were also up-regulated by BPE and panduratin A treatment. These results suggest that BPE and panduratin A are potential nutraceuticals which can enhance skin hydration and barrier function based on their CE formation and filaggrin processing.

키워드

참고문헌

  1. Kato E, Takahashi N. 2012. Improvement by sodium dl-${\alpha}$-tocopheryl-6-O-phosphate treatment of moisture-retaining ability in stratum corneum through increased ceramide levels. Bioorg Med Chem 20: 3837-3842. https://doi.org/10.1016/j.bmc.2012.04.029
  2. Wickett RR, Visscher MO. 2006. Structure and function of the epidermal barrier. Am J Infect Control 34: S98-S110. https://doi.org/10.1016/j.ajic.2006.05.295
  3. Rawlings AV, Harding CR. 2004. Moisturization and skin barrier function. Dermatol Ther 17: 43-48. https://doi.org/10.1111/j.1396-0296.2004.04S1005.x
  4. Kim HA, Lim YJ, Park JH, Cho YH. 2012. Dietary silk protein, sericin, improves epidermal hydration with increased levels of filaggrins and free amino acids in NC/Nga mice. Br J Nutr 108: 1726-1735. https://doi.org/10.1017/S0007114511007306
  5. Proksch E, Brandner JM, Jensen JM. 2008. The skin: an indispensable barrier. Exp Dermatol 17: 1063-1072. https://doi.org/10.1111/j.1600-0625.2008.00786.x
  6. Candi E, Schmidt R, Melino G. 2005. The cornified envelope: a model of cell death in the skin. Nat Rev Mol Cell Biol 6: 328-340. https://doi.org/10.1038/nrm1619
  7. Lim SW, Hong SP, Jeong SW, Kim BR, Bak HN, Ryoo HC, Lee SH, Ahn SK. 2007. Simultaneous effect of ursolic acid and oleanolic acid on epidermal permeability barrier function and epidermal keratinocyte differentiation via peroxisome proliferator-activated receptor-${\alpha}$. J Dermatol 34: 625-634. https://doi.org/10.1111/j.1346-8138.2007.00344.x
  8. Kim SH, Nam GW, Lee HK, Moon SJ, Chang IS. 2006. The effects of Musk T on peroxisome proliferator-activated receptor [PPAR]-${\alpha}$ activation, epidermal skin homeostasis and dermal hyaluronic acid synthesis. Arch Dermatol Res 298: 273-282. https://doi.org/10.1007/s00403-006-0684-y
  9. Hanley K, Jiang Y, He SS, Friedman M, Elias PM, Bikle DD, Williams ML, Feingold KR. 1998. Keratinocyte differentiation is stimulated by activators of the nuclear hormone receptor PPAR${\alpha}$. J Invest Dermatol 110: 368-375. https://doi.org/10.1046/j.1523-1747.1998.00139.x
  10. Sandilands A, Sutherland C, Irvine AD, McLean WH. 2009. Filaggrin in the frontline: role in skin barrier function and disease. J Cell Sci 122: 1285-1294. https://doi.org/10.1242/jcs.033969
  11. Brown SJ, McLean WHI. 2012. One remarkable molecule: filaggrin. J Invest Dermatol 132: 751-762. https://doi.org/10.1038/jid.2011.393
  12. McAleer MA, Irvine AD. 2013. The multifunctional role of filaggrin in allergic skin disease. J Allergy Clin Immunol 131: 280-291. https://doi.org/10.1016/j.jaci.2012.12.668
  13. Ovaere P, Lippens S, Vandenabeele P, Declercq W. 2009. The emerging roles of serine protease cascades in the epidermis. Trends Biochem Sci 34: 453-463. https://doi.org/10.1016/j.tibs.2009.08.001
  14. Nicotera P, Melino G. 2007. Caspase-14 and epidermis maturation. Nat Cell Biol 9: 621-622. https://doi.org/10.1038/ncb0607-621
  15. Kim MS, Pyun HB, Hwang JK. 2013. Effects of orally administered fingerroot (Boesenbergia pandurata) extract on oxazolone-induced atopic dermatitis-like skin lesions in hairless mice. Food Sci Biotechnol 22: 257-264.
  16. Shim JS, Kwon YY, Han YS, Hwang JK. 2008. Inhibitory effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) in dermal fibroblast cells. Planta Medica 72: 1446-1450.
  17. Kim SK, Oh HI, Hwang JK. 2012. Oral administration of fingerroot (Boesenbergia pandurata) extract reduces ultraviolet B-induced skin aging in hairless mice. Food Sci Biotechnol 21: 1753-1760. https://doi.org/10.1007/s10068-012-0233-8
  18. Hasegawa T, Shimada H, Uchiyama T, Ueda O, Nakashima M, Matsuoka Y. 2011. Dietary glucosylceramide enhances cornified envelope formation via transglutaminase expression and involucrin production. Lipids 46: 529-535. https://doi.org/10.1007/s11745-011-3546-0
  19. Zhou LL, Lin ZX, Fung KP, Che CT, Zhao M, Cheng CH, Zuo Z. 2012. Ethyl acetate fraction of Radix rubiae inhibits cell growth and promotes terminal differentiation in cultured human keratinocytes. J Ethnopharmacol 142: 241-247. https://doi.org/10.1016/j.jep.2012.04.051
  20. Kim MS, Oh GH, Kim MJ, Hwang JK. 2013. Fucosterol inhibits matrix metalloproteinase expression and promotes type-1 procollagen production in UVB-induced HaCaT cells. Photochem Photobiol 89: 911-918. https://doi.org/10.1111/php.12061
  21. Hou M, Man M, Man W, Zhu W, Hupe M, Park K, Crumrine D, Elias PM, Man MQ. 2012. Topical hesperidin improves epidermal permeability barrier function and epidermal differentiation in normal murine skin. Exp Dermatol 21: 337-340. https://doi.org/10.1111/j.1600-0625.2012.01455.x
  22. Kim D, Lee MS, Jo K, Lee KE, Hwang JK. 2011. Therapeutic potential of panduratin A, LKB1-dependent AMP-activated protein kinase stimulator, with activation of PPAR${\alpha}/{\delta}$ for the treatment of obesity. Diabetes Obes Metab 13: 584-593. https://doi.org/10.1111/j.1463-1326.2011.01379.x
  23. Kim MS, Pyun HB, Hwang JK. 2014. Panduratin A, an activator of PPAR-${\alpha}/{\delta}$, suppresses the development of oxazolone-induced atopic dermatitis-like symptoms in hairless mice. Life Sci 100: 45-54. https://doi.org/10.1016/j.lfs.2014.01.076

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