Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effects of 1-tetradecanol and β-sitosterol Isolated from Dendropanax morbifera Lev. on Skin Whitening, Moisturizing and Preventing Hair Loss

미백, 보습 및 탈모방지에 대한 황칠나무(Dendropanax modifera Lev.)에서 분리한 1-tetradecanol, β-sitosterol의 효과

  • Lee, Sun Young (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR)) ;
  • Choi, Eun-Jin (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR)) ;
  • Bae, Dong-Hyuck (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR)) ;
  • Lee, Dong-Wook (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR)) ;
  • Kim, Sunoh (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR))
  • 이선영 ((재)전남생물산업진흥원 천연자원연구원) ;
  • 최은진 ((재)전남생물산업진흥원 천연자원연구원) ;
  • 배동혁 ((재)전남생물산업진흥원 천연자원연구원) ;
  • 이동욱 ((재)전남생물산업진흥원 천연자원연구원) ;
  • 김선오 ((재)전남생물산업진흥원 천연자원연구원)
  • Received : 2015.02.10
  • Accepted : 2015.03.13
  • Published : 2015.03.31
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Abstract

Dendropanax morbifera Leveille (Araliaceae) is an endemic species growing in the south-western part of South Korea and has been used in folk medicine. However, the effects of Dendropanax morbifera Lev. on skin biology remain to be elucidated. In this study, we isolated 1-tetradecanol and ${\beta}$-sitosterol from the n-hexane fraction of Dendropanax mobifera Lev. and To investigate the whitening effect of the fraction, we tested the inhibition of tyrosinase activity of 1-tetradecanol. The results show that the inhibitory effect of the 1-tetradecanol was higher than water extract and n-hexane fraction. And 1-tetradecanol significantly reduced melanin contents of B16F10 cells compared to more than water extract and n-haxane fraction dose-dependantly without cell cytotoxicitiy (below $100{\mu}g/mL$). We also investigated the skin moisturizing effect using HR-1 hairless mice. The transepidermal water loss (TEWL) in the 1-tetradecanol treated group was significantly smaller than that in the other groups. To investigate the effect of the preventing hair loss by ${\beta}$-sitosterol, we observed HR-1 hairless mice through periodic growth feature. The results suggest that hair loss of mice by ${\beta}$-sitosterol was delayed and it's hair density showed the highest. These data provide evidence that Dendropanax morbifera Lev. may be a potent candidate for the improvement of both skin whitening, moisturizing and alopecia from the point of cosmetic industry view.

황칠나무(Dendropanax mobifera Lev.)는 두릅나무과에 속하는 난대성 수종으로 대한민국 남쪽지방에 주로 분포하며 전통적인 민간요법 약재로 사용되어 왔으나 지금까지 피부에서의 효능이 잘 알려지지 않았다. 우리는 본 연구에서 황칠나무 잎의 열수추출물과 n-hexane 분획물에서 1-tetradecanol과 ${\beta}$-sitosterol 성분을 분리정제하여 다양한 피부개선효과와 탈모방지효과를 검증하였다. 1-tetradecanol의 타이로시나아제 활성 저해 효과는 열수추출물과 n-hexane분획물 보다 높은 저해 활성이 나타났다. 그리고 B16F10 세포를 이용한 멜라닌 양을 측정한 결과 1-tetradecanol은 세포독성 없이 농도 의존적으로 멜라닌 양이 감소됨을 확인하였다. 실험동물을 이용한 경표피수분손실량(TEWL)에서도 1-tetradecanol에서 수분 손실이 가장 낮게 나타났다. ${\beta}$-sitosterol의 탈모 방지에 대한 효과는 HR-1 hairless mice의 성장 주기별 특성을 이용하여 관찰하였다. 그 결과 ${\beta}$-sitosterol을 처리한 마우스의 탈모는 지연되었고 모발의 밀도도 가장 높게 나타났다. 그러므로 황칠 n-hexane분획물에서 분리한 1-tetradecanol과 ${\beta}$-sitosterol이 미백과 보습 용도의 화장품 기능성 원료 및 탈모 개선에 가능성이 있는 화장품소재로서 상업적 발전의 가능성을 보여주고 있다.

Keywords

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