생약학회지 (Korean Journal of Pharmacognosy)

  • 제46권4호
  • /
  • Pages.295-302
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  • 2015
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

백하수오(Cynanchi Wilfordii Radix)의 Cynandione A가 RAW 264.7 세포에서 RANKL과 LPS로 유도된 파골세포형성에 대한 영향

Effect of Cynandione A of Cynanchi Wilfordii Radix in RANKL and Lipopolysaccharide-induced on Osteoclastogeneis in RAW 264.7 Cells

  • 황준호 (제주대학교 생명과학기술혁신센터) ;
  • 이미란 (제주대학교 생명과학기술혁신센터) ;
  • 강창희 (제주대학교 생명과학기술혁신센터) ;
  • 부희정 (제주대학교 생명과학기술혁신센터)
  • Hwang, Joon-Ho (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Yi, Mi-Ran (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Kang, Chang-Hee (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Bu, Hee-Jung (Biotechnology Regional Innovation Center, Jeju National University)
  • 투고 : 2015.11.25
  • 심사 : 2015.12.15
  • 발행 : 2015.12.31
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초록

Cynanchi wilfordii Radix roots have been utilized as traditional medicine for variety of diseases including diabetes mellitus, aging progression and scavenging free radicals, enhancing immunity, reducing high serum cholesterol, and anti-tumor activity. However, the mechanisms underlying this effect remain poorly understood. The principal objective of this study was to determine the effect of cynandione A on osteoclast cells. Thus, we was isolated cynandione A from Cynanchi wilfordii Radix roots and evaluated the effect of cynandione A on receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. We found that cynandione A significantly inhibited osteoclast differentiation stimulated-RANKL in RAW 264.7 cells. Cynandione A conspicuously inhibited the mRNA and protein expression of matrix metallopeptidase 9 (MMP-9), tartrate-resistant acid phosphatase (TRAP) in cynandione A treated with RANKL. Taken together, our results demonstrated that Cynanchi Wilfordii Radix may be useful treatment option of bone-related disease such as osteoporosis leads to fracture of bone and rheumatoid arthritis.

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