Biomaterials and Biomechanics in Bioengineering

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Temperature-responsive bioactive hydrogels based on a multifunctional recombinant elastin-like polymer

  • Santo, Vitor E. (G.I.R. Bioforge, Centro I+D, Campus Miguel Delibes, University of Valladolid) ;
  • Prieto, Susana (G.I.R. Bioforge, Centro I+D, Campus Miguel Delibes, University of Valladolid) ;
  • Testera, Ana M. (G.I.R. Bioforge, Centro I+D, Campus Miguel Delibes, University of Valladolid) ;
  • Arias, Francisco J. (G.I.R. Bioforge, Centro I+D, Campus Miguel Delibes, University of Valladolid) ;
  • Alonso, Matilde (G.I.R. Bioforge, Centro I+D, Campus Miguel Delibes, University of Valladolid) ;
  • Mano, Joao F. (3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Dept. of Polymer Engineering, University of Minho) ;
  • Rodriguez-Cabello, Jose Carlos (G.I.R. Bioforge, Centro I+D, Campus Miguel Delibes, University of Valladolid)
  • Received : 2014.12.30
  • Accepted : 2015.02.20
  • Published : 2015.03.25
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Abstract

A bioactive and multifunctional elastin-like polymer (ELP) was produced by genetic engineering techniques to develop new artificial matrices with the ability to mimic the extracellular matrix (ECM). The basic composition of this ELP is a thermo- and pH-sensitive elastin pentapeptide which has been enriched with RGD-containing domains, the RGD loop of fibronectin, for recognition by integrin receptors on their sequence to promote efficient cell attachment. Hydrogels of this RGD-containing polymer were obtained by crosslinking with hexamethylene diisocyanate, a lysine-targeted crosslinker. These materials retain the "smart" nature and temperature-responsive character, and the desired mechanical behavior of the elastin-like polymer family. The influence of the degree of crosslinking on the morphology and properties of the matrices were tested by calorimetric techniques and scanning electron microscopy (SEM). Their mechanical behavior was studied by dynamical mechanical analysis (DMA). These results show the potential of these materials in biomedical applications, especially in the development of smart systems for tissue engineering.

Keywords

References

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