한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제60권1호
  • /
  • Pages.29-32
  • /
  • 2015
  • /
  • 0252-9777(pISSN)
  • /
  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
권호 표지
DOI QR코드

DOI QR Code

Proteome Approach as a Tool for the Efficient Separation of Seed Storage Proteins from Buckwheat

  • 투고 : 2014.12.26
  • 심사 : 2015.02.09
  • 발행 : 2015.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.

키워드

참고문헌

  1. Dolinsek, B. 1980. Studies of polymorphism of electrophoretic proteins in buckwheat. Proceeding of 1st Buckwheat Symp., Ljubljana : 61-68.
  2. Gorg et al. 1992. Two-dimensional polyacrylamide gel electrophoresis, with immobilized pH gradients in the first dimension, of barley seed proteins: Discrimination of cultivars with different malting grades. Electrophoresis 13 : 192. https://doi.org/10.1002/elps.1150130141
  3. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680-685. https://doi.org/10.1038/227680a0
  4. Nair, A. and T. Adachi. 1999. Immunodetection and characterization of allergenic proteins in common buckwheat (Fagopyrum esculentum). Plant Biotech. 16 : 219-224. https://doi.org/10.5511/plantbiotechnology.16.219
  5. Nishiyama, K., S. Lachmann, and M. Miura. 1991. Electrophoretic property of buckwheat seed proteins. Proceed. of ICOBB, Miyazaki : 215-222.
  6. O' Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250 : 4007-4021.
  7. Ohnishi, O. 1998. Search for the wild ancestor of buckwheat. I. Description of new Fagopyrum (Polygonaceae) species and their distribution in China and Himalayan hills. Fagopyrum 15 : 18-28.
  8. Pandy, A. and M. Mann. 2000. Proteomics to study genes and genomes. Nature 405 : 837-846. https://doi.org/10.1038/35015709
  9. Shevchuk, T. E. 1986. The polymorphism of buckwheat 13s globulin. Fagopyrum 6 : 9.
  10. Svetek, S. 1994. Electrophoretic analysis of buckwheat (Fagopyrum esculentum Moench) seed proteins. Porch. of IPBA, Rogla : 161-170.
  11. Svetlana, R. R., V. R. Maksimovic, and E. I. Varkonji-Gasic. 1996. Characterization of Buckwheat Seed Storage Proteins. J. Agric. Food. Chem. 44 : 972-974. https://doi.org/10.1021/jf950655x
  12. Tahir, I. and T. Farooq. 1988. Review article on buckwheat. Fagopyrum 8 : 33-53.
  13. Urisu, A., Y. Kondo, Y. Morita, E. Wada, M. Tsuruta, T. Yasaki, K. Yamada, H. Kuzaya, M. Suzuki, K. Titani, and K. Kurosawa. 1994. Identification of a major allergen of buckwheat seeds by immunoblotting methods. Allerg. Clin. Immunol. 6 : 151-155.
  14. Woo, S. H., M. Fukuda, N. Islam, M. Takaoka, H. Kawasaki, and H. Hirano. 2002. Efficient peptide mapping and its application to identification of embryo proteins in the rice proteome analysis. Electrophoresis, 23 : 647-654. https://doi.org/10.1002/1522-2683(200202)23:4<647::AID-ELPS647>3.0.CO;2-O