Journal of Korean Ophthalmic Optics Society (한국안광학회지)

The Korean Ophthalmic Optics Society (한국안광학회)
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Cytotoxicity of Multipurpose Contact Lens Solutions on the Cultured Corneal Epithelial Cells Evaluated by Image Analysis

이미지 분석법을 이용한 소프트 콘택트렌즈용 다목적용액의 각막상피세포 독성 평가

  • Kim, Nam-Youl (Graduate School of Public Health Science, Eulji University) ;
  • Lee, Koon-Ja (Graduate School of Public Health Science, Eulji University)
  • 김남열 (을지대학교 대학원 보건학과) ;
  • 이군자 (을지대학교 대학원 보건학과)
  • Received : 2015.02.03
  • Accepted : 2015.03.24
  • Published : 2015.03.31
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Abstract

Purpose: To determine the effect of marketed multipurpose contact lens solutions (MPSs) on human corneal epithelial cells (HCEpiCs) toxicity by using image analysis. Methods: HCEpiCs were exposed six MPSs (product A-F) at 0.05~50% for 2h, 12h, 24h, and 48h respectively. HCEpiCs were fixed and stained with Draq5 after exposure with MPSs, and the cell viability and apoptosis were evaluated by using confocal microscope and ImageXpress UltraTM. Results: Viabilities of HCEpiCs exposed to MPS A-F for a 2h were not affected, while reductions (52~75%) in cell viability over a 12h exposure of MPS B, MPS C, MPS D and MPS F, and significant more reductions (29~73%) over a 24h and 48h-exposure. Apoptosis of HCEpiC was not affect over a 12h MPS exposure, however was significantly increased (199~526%) over 24h and 48h MPS exposure. Among the products MPS D, E and F reduced viability of HCEpiCs and apoptosis increased more than MPS A (p<0.05). Conclusions: Lower concentration of MPSs have not an cytotoxic effect on HCEpiCs, however higher concentration of MPSs induce apoptosis and reduce viability of HCEpiCs. Therefore, it need to develop MPS having antimicrobial effectiveness with low cytotoxicity.

목적: 시판되고 있는 소프트콘택트렌즈용 다목적용액이 사람의 각막상피세포에 미치는 세포독성을 이미지 분석법을 이용하여 평가하고자 하였다. 방법: 각막상피세포를 6종류의 다목적용액(A~F)이 0.05~50% 포함된 배양액에서 각각 2시간, 12시간, 24시간, 48시간 동안 배양하였다. 배양 후 각막상피세포를 고정한 다음 Draq 5로 염색하고 공초점현미경과 ImageXpress $Ultra^{TM}$를 이용하여 세포형태를 관찰하고 세포생존율과 세포자살(apoptosis)을 비교하였다. 결과: 각막상피세포 생존율과 세포자살은 다목적용액을 2시간 처리한 경우에는 모든 제품에서 대조군과 차이가 없었으나, 12시간 이상 처리한 경우에는 B~F 제품에서 대조군의 52~75% 수준으로 감소하였고, 세포자살은 모든 제품에서 차이가 없었다. 24~48시간 처리한 경우에는 생존율이 29~73% 수준으로 감소하였고(p<0.05), 세포자살은 199~526% 증가하였다. 제품별로는 다목적용액 D, E, F가 A보다 각막상피세포 생존율을 감소시켰고 세포자살을 증가시켰다(p<0.05). 결론: 저농도의 다목적용액은 각막상피세포 독성에 영향을 미치지 않지만 고농도의 다목적용액은 각막상피세포의 자살을 유도하고 세포생존율을 저하시킬 수 있으므로 다목적용액의 성분은 소독기능이 우수하고 독성이 없는 성분으로 개발되어야 할 것으로 사료된다.

Keywords

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