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초임계 이산화탄소를 이용한 Nannochloropsis sp. 미세조류로부터 바이오디젤 생산용 지질의 추출

Lipid Extraction from Nannochloropsis sp. Microalgae for Biodiesel Production Using Supercritical Carbon Dioxide

  • 최경석 ((주)한울엔지니어링 기술연구소) ;
  • 류재훈 (한양대학교 화학공학과) ;
  • 박동준 ((주)한울엔지니어링 기술연구소) ;
  • 오세천 (공주대학교 환경공학과) ;
  • 곽현 ((주)한울엔지니어링 기술연구소)
  • 투고 : 2014.06.24
  • 심사 : 2014.08.17
  • 발행 : 2015.04.01

초록

본 연구에서는 미세조류인 Nannochloropsis sp.로부터 바이오디젤 생산용 지질을 얻기 위하여 유기용매 및 초임계 이산화탄소(SC-$CO_2$)를 이용하여 추출을 수행하였다. SC-$CO_2$ 추출법으로 얻은 지질의 지방산메틸에스테르 함량은 58.31%로 높았으며, Bligh-Dyer 추출법은 18.0 wt.%의 가장 높은 조지방 수율을 나타내었다. SC-$CO_2$ 추출법에 극성을 높이기 위해 공용매로서 methanol을 사용한 결과, 조지방 수율 12.5 wt.%, 지방산메틸에스테르 함량 56.32%, 지방산메틸에스테르 수율 7.04 wt.%였으며, SC-$CO_2$ 만을 이용하는 추출 방법에 비하여 추출 시간을 2시간에서 30분으로 단축시킬 수 있었다. 따라서 미세조류에서 지질을 추출하는데 기존의 유기용매 추출법과 비교하여 SC-$CO_2$ 추출법이 친환경적이며, 효율적인 방법임을 확인하였다.

In this paper, microalgae lipid extractions were performed using conventional organic solvent and supercritical carbon dioxide (SC-$CO_2$) for biodiesel-convertible lipid fractions. The highest levels (58.31%) of fatty acid methyl ester (FAME) content in the lipid extracted by SC-$CO_2$ was obtained, and 18.0 wt.% crude lipid yield was achieved for Bligh-Dyer method. In the SC-$CO_2$ extraction, methanol as a co-solvent was applied to increase the polarity of extract. The experimental results indicated that crude lipid yield, FAME content and yield extracted by combination of SC-$CO_2$ with methanol were 12.5 wt.%, 56.32% and 7.04 wt.%, respectively, and this method could reduce the extraction time from 2 hour to 30 min when compared to SC-$CO_2$ extraction. Therefore, SC-$CO_2$ extraction is proven to be an environmentally-friendly and an effective method for lipid extraction from microalgae.

키워드

참고문헌

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피인용 문헌

  1. 유기발광소자를 위한 해양 미세조류 유래 물질 및 광 발광 탐색 vol.29, pp.4, 2015, https://doi.org/10.13047/kjee.2015.29.4.564
  2. 탈지미세조류의 무효소 당화를 위한 마이크로파 전처리 조건 최적화 vol.56, pp.2, 2015, https://doi.org/10.9713/kcer.2018.56.2.229