생체재료학회지 (Biomaterials Research)

  • 제19권3호
  • /
  • Pages.134-142
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  • 2015
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
DOI QR코드

DOI QR Code

Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration

  • Kim, Beom-Su (Wonkwang Bone Regeneration Research Institute, Wonkwang University) ;
  • Kim, Jin-Seong (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Yang, Sun-Sik (Wonkwang Bone Regeneration Research Institute, Wonkwang University) ;
  • Kim, Hyung-Woo (Department of Dentistry, Oral and Maxillofacial, Wonkwang University) ;
  • Lim, Hun Jun (Department of Dentistry, Oral and Maxillofacial, Wonkwang University) ;
  • Lee, Jun (Wonkwang Bone Regeneration Research Institute, Wonkwang University)
  • 투고 : 2015.07.22
  • 심사 : 2015.08.04
  • 발행 : 2015.09.30
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Angiogenin (ANG) is a potent stimulator of angiogenesis. The aim of this study was to fabricate an ANG-loaded scaffold and to evaluate its angiogenic and osteogenic effects. In this study, we fabricated an ANG-loaded scaffold using bovine bone powder and fibrin glue. We then evaluated the structural, morphological, and mechanical properties of the scaffold and the in vitro release profile of ANG. Cell proliferation, viability, and adhesion were evaluated using endothelial cells in vitro, and angiogenesis and new bone formation were evaluated using a rabbit calvarial defect model in vivo. Results: Micro-computed tomography imaging showed that the bone powder was uniformly distributed in the scaffold, and scanning electron microscopy showed that the bone powder was bridged by polymerized fibrin. The porosity and compressive strength of the scaffolds were ~60 % and ~0.9 MPa, respectively, and were not significantly altered by ANG loading. In vitro, at 7 days, approximately $0.4{\mu}g$ and $1.3{\mu}g$ of the ANG were released from the FB/ANG 0.5 and FB/ANG 2.0, respectively and sustained slow release was observed until 25 days. The released ANG stimulated cell proliferation and adherence and was not cytotoxic. Furthermore, in vivo implantation resulted in enhanced angiogenesis, and new bone formation depended on the amount of loaded ANG. Conclusions: These studies demonstrate that a fibrin and bone powder scaffold loaded with ANG might be useful to promote bone regeneration by enhanced angiogenesis.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea (NRF)

참고문헌

  1. Kuboki Y, Jin Q, Takita H. Geometry of carriers controlling phenotypic expression in BMP-induced osteogenesis and chondrogenesis. J Bone Joint Surg Am. 2001;83-A Suppl 1:S105-15.
  2. Murata M, Sato D, Hino J, Akazawa T, Tazaki J, Ito K, et al. Acid-insoluble human dentin as carrier material for recombinant human BMP-2. J Biomed Mater Res A. 2012;100:571-7.
  3. Hankenson KD, Dishowitz M, Gray C, Schenker M. Angiogenesis in bone regeneration. Injury. 2011;42:556-61. https://doi.org/10.1016/j.injury.2011.03.035
  4. Ozturk BY, Inci I, Egri S, Ozturk AM, Yetkin H, Goktas G, et al. The treatment of segmental bone defects in rabbit tibiae with vascular endothelial growth factor (VEGF)-loaded gelatin/hydroxyapatite "cryogel" scaffold. J Pediatr Orthop B. 2013;23:767-74.
  5. Devescovi V, Leonardi E, Ciapetti G, Cenni E. Growth factors in bone repair. Chir Organi Mov. 2008;92:161-8. https://doi.org/10.1007/s12306-008-0064-1
  6. Fett JW, Strydom DJ, Lobb RR, Alderman EM, Bethune JL, Riordan JF, et al. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells. Biochemistry (Mosc). 1985;24:5480-6. https://doi.org/10.1021/bi00341a030
  7. Kishimoto K, Liu S, Tsuji T, Olson KA, Hu GF. Endogenous angiogenin in endothelial cells is a general requirement for cell proliferation and angiogenesis. Oncogene. 2005;24:445-56. https://doi.org/10.1038/sj.onc.1208223
  8. Hatano N, Shimizu Y, Ooya K. A clinical long-term radiographic evaluation of graft height changes after maxillary sinus floor augmentation with a 2:1 autogenous bone/xenograft mixture and simultaneous placement of dental implants. Clin Oral Implants Res. 2004;15:339-45. https://doi.org/10.1111/j.1600-0501.2004.00996.x
  9. Yao PP, Sung CY. Securinine metabolism. Zhonghua yi xue za zhi. 1973;4:229-35.
  10. Thevis M, Sigmund G, Gougoulidis V, Beuck S, Schlorer N, Thomas A, et al. Screening for benfluorex and its major urinary metabolites in routine doping controls. Anal Bioanal Chem. 2011;401:543-51. https://doi.org/10.1007/s00216-010-4455-4
  11. Portha B, Serradas P, Bailbe D, Blondel O, Picarel F. Effect of benfluorex on insulin secretion and insulin action in streptozotocin-diabetic rats. Diabetes Metab Rev. 1993;9 Suppl 1:57S-63. https://doi.org/10.1002/dmr.5610090510
  12. IIa B. Pharmacology of alkaloid securinine. Farmakol Toksikol. 1956;19:3-5.
  13. Clark RA, Lanigan JM, DellaPelle P, Manseau E, Dvorak HF, Colvin RB. Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelialization. J Invest Dermatol. 1982;79:264-9. https://doi.org/10.1111/1523-1747.ep12500075
  14. Boudes A, Lavoute C, Avierinos JF, Le Dolley Y, Villacampa C, Salem A, et al. Valvular heart disease associated with benfluorex therapy: high prevalence in patients with unexplained restrictive valvular heart disease. Eur J Echocardiogr. 2011;12:688-95. https://doi.org/10.1093/ejechocard/jer116
  15. Bondon-Guitton E, Prevot G, Didier A, Montastruc JL. Pulmonary arterial hypertension and benfluorex: 5 case reports. Therapie. 2011;66:135-8. https://doi.org/10.2515/therapie/2011018
  16. Breen A, O'Brien T, Pandit A. Fibrin as a delivery system for therapeutic drugs and biomolecules. Tissue Eng Part B. 2009;15:201-14.
  17. Borden M, El-Amin SF, Attawia M, Laurencin CT. Structural and human cellular assessment of a novel microsphere-based tissue engineered scaffold for bone repair. Biomaterials. 2003;24:597-609. https://doi.org/10.1016/S0142-9612(02)00374-5
  18. Stephan SJ, Tholpady SS, Gross B, Petrie-Aronin CE, Botchway EA, Nair LS, et al. Injectable tissue-engineered bone repair of a rat calvarial defect. Laryngoscope. 2010;120:895-901.
  19. Hing KA. Bone repair in the twenty-first century: biology, chemistry or engineering? Philos Transact A Math Phys Eng Sci. 2004;362:2821-50. https://doi.org/10.1098/rsta.2004.1466
  20. Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005;26:5474-91. https://doi.org/10.1016/j.biomaterials.2005.02.002
  21. Su J, Xu H, Sun J, Gong X, Zhao H. Dual Delivery of BMP-2 and bFGF from a New Nano-Composite Scaffold, Loaded with Vascular Stents for Large-Size Mandibular Defect Regeneration. Int J Mol Sci. 2013;14:12714-28. https://doi.org/10.3390/ijms140612714
  22. Luo T, Zhang W, Shi B, Cheng X, Zhang Y. Enhanced bone regeneration around dental implant with bone morphogenetic protein 2 gene and vascular endothelial growth factor protein delivery. Clin Oral Implants Res. 2012;23:467-73. https://doi.org/10.1111/j.1600-0501.2011.02164.x
  23. Gomez G, Korkiakoski S, Gonzalez MM, Lansman S, Ella V, Salo T, et al. Effect of FGF and polylactide scaffolds on calvarial bone healing with growth factor on biodegradable polymer scaffolds. J Craniofac Surg. 2006;17:935-42. https://doi.org/10.1097/01.scs.0000231624.87640.55
  24. Kim BS, Sung HM, You HK, Lee J. Effects of fibrinogen concentration on fibrin glue and bone powder scaffolds in bone regeneration. J Biosci Bioeng. 2014;118:469-75. https://doi.org/10.1016/j.jbiosc.2014.03.014
  25. Rowe SL, Stegemann JP. Interpenetrating collagen-fibrin composite matrices with varying protein contents and ratios. Biomacromolecules. 2006;7:2942-8. https://doi.org/10.1021/bm0602233
  26. Leach JK, Kaigler D, Wang Z, Krebsbach PH, Mooney DJ. Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration. Biomaterials. 2006;27:3249-55. https://doi.org/10.1016/j.biomaterials.2006.01.033
  27. Jeon O, Ryu SH, Chung JH, Kim BS. Control of basic fibroblast growth factor release from fibrin gel with heparin and concentrations of fibrinogen and thrombin. J Control Release. 2005;105:249-59. https://doi.org/10.1016/j.jconrel.2005.03.023
  28. Liu S, Yu D, Xu ZP, Riordan JF, Hu GF. Angiogenin activates Erk1/2 in human umbilical vein endothelial cells. Biochem Biophys Res Commun. 2001;287:305-10. https://doi.org/10.1006/bbrc.2001.5568
  29. Soncin F. Angiogenin supports endothelial and fibroblast cell adhesion. Proc Natl Acad Sci U S A. 1992;89:2232-6. https://doi.org/10.1073/pnas.89.6.2232
  30. Kaigler D, Wang Z, Horger K, Mooney DJ, Krebsbach PH. VEGF scaffolds enhance angiogenesis and bone regeneration in irradiated osseous defects. J Bone Miner Res. 2006;21:735-44. https://doi.org/10.1359/jbmr.060120
  31. [Peculiarities of hepatic experimental wound healing in rats treated with angiogenin]. Morfologiia. 2005;128:98-100.
  32. King TV, Vallee BL. Neovascularisation of the meniscus with angiogenin. An experimental study in rabbits. J Bone Joint Surg Br. 1991;73:587-90. https://doi.org/10.2106/00004623-199173040-00016
  33. Shi H, Han C, Mao Z, Ma L, Gao C. Enhanced angiogenesis in porous collagen-chitosan scaffolds loaded with angiogenin. Tissue Eng Part A. 2008;14:1775-85. https://doi.org/10.1089/ten.tea.2007.0007

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