생체재료학회지 (Biomaterials Research)

  • 제19권2호
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  • Pages.106-116
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  • 2015
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
DOI QR코드

DOI QR Code

Synthesis and in vitro characterizations of porous carboxymethyl cellulose-poly(ethylene oxide) hydrogel film

  • Lee, Su Yeon (Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology) ;
  • Bang, Sumi (Convergence Institute of Biomedical Engineering and Biomaterials, Seoul National University of Science and Technology) ;
  • Kim, Sumi (Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology) ;
  • Jo, Seong Yeon (Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology) ;
  • Kim, Bum-Chul (Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology) ;
  • Hwang, Yunjae (Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology) ;
  • Noh, Insup (Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology)
  • 투고 : 2015.02.09
  • 심사 : 2015.04.03
  • 발행 : 2015.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Cellulose and its derivatives such as carboxymethyl cellulose (CMC) have been employed as a biomaterial for their diverse applications such as tissue engineering, drug delivery and other medical materials. Porosity of the scaffolds has advantages in their applications to tissue engineering such as more cell adhesion and migration leading to better tissue regeneration. After synthesis of CMC-poly(ethylene oxide) (PEO) hydrogel by mixing the solutions of both CMC-acrylate and PEO-hexa-thiols, fabrication and evaluation of a CMC-PEO gel and its film in porous form have been made for its possible applications to tissue regeneration. Physicochemical and biological properties of both CMC-PEO hydrogel and porous films have been evaluated by using physicochemical assays by SEM, FTIR and swelling behaviors as well as in vitro assays of MTT, Neutral red, BrdU, gel covering and tissue ingrowth into the pores of the CMC-PEO gel films. Degradation of CMC-PEO hydrogel was also evaluated by treating with esterase over time. Results: Chemical grafting of acrylate to CMC was verified by analyses of both FTIR and NMR. CMC-PEO hydrogel was obtained by mixing two precursor polymer solutions of CMC-acrylate and PEO-hexa-thiols and by transforming into a porous CMC-PEO gel film by gas forming of ammonium bicarbonate particles. The fabricated hydrogel has swollen in buffer to more than 6 times and degraded by esterase. The results of in vitro assays of live and dead, MTT, BrdU, Neutral red and gel covering on the cells showed excellent cell compatibility of CMC-PEO hydrogel and porous gel films. Furthermore the porous films showed excellent in vitro adhesion and migration of cells into their pore channels as observed by H&E and MT stains. Conclusions: Both CMC-PEO hydrogel and porous gel films showed excellent biocompatibility and were expected to be a good candidate scaffold for tissue engineering.

키워드

참고문헌

  1. Ramli NA, Wong TW. Sodium carboxymethyl cellulose scaffolds and their physicochemical effects on partial thickness wound healing. Intl J Pharm. 2011;403(1-2):73-82. https://doi.org/10.1016/j.ijpharm.2010.10.023
  2. Dinarvand R, Khodaverdi E, Atyabi F, Erfan M. Thermoresponsive drug delivery using liquid crystal-embedded cellulose nitrate membranes. Drug Deliv. 2006;13(5):345-50. https://doi.org/10.1080/10717540500394729
  3. Nouran El Badawi NE, Ramadan AR, Esawi AM, El-Morsi M. Novel carbon nanotube-cellulose acetate nanocomposite membranes for water filtration applications. Desalination. 2014;344:79-85. https://doi.org/10.1016/j.desal.2014.03.005
  4. Kang H, Liu R, Huang Y. Cellulose derivatives and graft copolymers as blocks for functional materials. Polym Intl. 2033;62(3):338-44.
  5. Vlierberghe SV, Dubruel P, Schacht E. Biopolymer-based hydrogels as scaffolds for tissue engineering applications: a review. Biomacromolecules. 2011;12:1387-408. https://doi.org/10.1021/bm200083n
  6. Gariepy ER, Leroux JC. In situ-forming hydrogels-review of temperaturesensitive systems. Eur J Pharm Biopharm. 2004;58:409-26. https://doi.org/10.1016/j.ejpb.2004.03.019
  7. Ramanan RM, Chellamuthu P, Tang L, Nguyen KT. Development of a temperature-sensitive composite hydrogel for drug delivery applications. Biotechnol Prog. 2006;22:118-25. https://doi.org/10.1021/bp0501367
  8. Kim AR, Park HS, Kim SS, Noh I. Biological evaluation of cellulose hydrogel with temperature-responsive particles. Biomater Res. 2013;17(4):181-6.
  9. Yang MB, Vacanti JP, Ingber DE. Hollow fibers for hepatocyte encapsulation and transplantation-studies of survival and function in rats. Cell Transplant. 1994;3(5):373-85. https://doi.org/10.1177/096368979400300504
  10. Risbud MV, Bhonde RR. Suitability of cellulose molecular dialysis membrane for bioartificial pancreas: in vitro biocompatibility studies. J Biomed Mater Res. 2001;54(3):436-44. https://doi.org/10.1002/1097-4636(20010305)54:3<436::AID-JBM180>3.0.CO;2-8
  11. Cullen B, Watt PW, Lundqvist C, Silcock D, Schmidt RJ, Bogan D, et al. The role of oxidized regenerated cellulose/collagen in chronic wound repair and its potential mechanism of action. Int J Biochem Cell Biol. 2002;34(12):1544-56. https://doi.org/10.1016/S1357-2725(02)00054-7
  12. Ovington LG. Overview of matrix metalloprotease modulation and growth factor protection in wound healing. Part 1. Ostomy Wound Manage. 2002;48(6):3-7.
  13. Miyamoto T, Takahashi S, Ito H, Inagaki H, Noishiki Y. Tissue biocompatibility of cellulose and its derivatives. J Biomed Mater Res. 1989;23(1):125-33. https://doi.org/10.1002/jbm.820230110
  14. Entcheva E, Bien H, Yin L, Chung CY, Farrell M, Kostov Y. Functional cardiac cell constructs on cellulose-based scaffolding. Biomaterials. 2004;25:5753-62. https://doi.org/10.1016/j.biomaterials.2004.01.024
  15. Xing Q, Zhao F, Chen S, McNamara J, DeCoster MA, Lvov YM. Porous biocompatible three-dimensional scaffolds of cellulose microfiber/gelatin composites for cell culture. Acta Biomater. 2010;6:2132-9. https://doi.org/10.1016/j.actbio.2009.12.036
  16. Fukuya MN, Senoo K, Kotera M, Yoshimoto M, Sakata O. Enhanced oxygen barrier property of poly(ethylene oxide) films crystallite-oriented by adding cellulose single nanofibers. Polymer. 2014;55(25):5843-6. https://doi.org/10.1016/j.polymer.2014.09.003
  17. Niyas AMI, Sankar S, Mohammed KP, Hayath BSK, Sastry TP. Evaluation of biomaterial containing regenerated cellulose and chitosan incorporated with silver nanoparticles. Int J Biol Macromol. 2015;72:680-6. https://doi.org/10.1016/j.ijbiomac.2014.08.055
  18. Gabriel MO, Marcio LS, Paula BD, Pierre B, Gildasio dCD, Antonio CG. Bacterial cellulose/chondroitin sulfate for dental materials scaffolds. J Biomater Tissue Eng. 2014;4(2):150-4. https://doi.org/10.1166/jbt.2014.1155
  19. Mikos AG, Sarakinos G, Leite SM, Vacanti JP, Langer R. Laminated three-dimensional biodegradable foams for use in tissue engineering. Biomaterials. 1993;14(5):323-30. https://doi.org/10.1016/0142-9612(93)90049-8
  20. Sevillano G, Rodriguez-Puyol M, Martos R, Duque I, Lamas S, Diez-Marques ML, et al. Cellulose acetate membrane improves some aspects of red blood cell function in haemodialysis patients. Nephrol Dial Transplant. 1990;5(7):497-9. https://doi.org/10.1093/ndt/5.7.497
  21. Chang Q, Murtaza Z, Lakowicz JR, Rao G. A: Fluorescence lifetime-based solid sensor for water. Anal Chim Acta. 1997;350(1-2):97-104. https://doi.org/10.1016/S0003-2670(97)00298-5
  22. Doheny JG, Jervis EJ, Guarna MM, Humphries RK, Warren RAJ, Kilburn DG. Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein. Biochem J. 1999;339:429-34.
  23. Ko IK, Iwata H. An approach to constructing three-dimensional tissue Bioartif Org Iii: Tissue Sourcing, Immunoisolation. Clin Trials. 2001;944:443-55.
  24. Takata T, Wang HL, Miyauchi M. Migration of osteoblastic cells on various guided bone regeneration membranes. Clin Oral Impl Res. 2001;12(4):332-8. https://doi.org/10.1034/j.1600-0501.2001.012004332.x
  25. De Bartolo L, Morelli S, Baer A, Drioli E. Evaluation of cell behavior related to physic-chemical properties of polymeric membranes to be used in bioartificial organs. Biomaterials. 2002;23(12):2485-97. https://doi.org/10.1016/S0142-9612(01)00383-0
  26. Kino Y, Sawa M, Kasai S, Mito M. Multiporous cellulose microcarrier for the development of a hybrid artificial liver using isolated hepatocytes. J Surg Res. 1998;79(1):71-6. https://doi.org/10.1006/jsre.1998.5389
  27. Martson M, Viljanto J, Hurme T, Saukko P. Biocompatibility of cellulose sponge with bone. Eur Surg Res. 1998;30(6):426-32. https://doi.org/10.1159/000008609
  28. Martson M, Viljanto J, Hurme T, Laippala P, Saukko P. Is cellulose sponge degradable or stable as implantation material? An in vivo subcutaneous study in the rat. Biomaterials. 1999;20(21):1989-95. https://doi.org/10.1016/S0142-9612(99)00094-0
  29. Kim MS, Choi YJ, Noh I, Tae G. Synthesis and characterization of in situ chitosan-based hydrogel via grafting of carboxyethyl acrylate. J Biomed Mater Res Part A. 2007;83(3):674-81.
  30. Falcone SJ, Doerfler AM, Berg RA. Novel synthetic dermal fillers based on sodium carboxymethyl cellulose: comparison with crosslinked hyaluronic acid-based dermal fillers. Dermatol Surg. 2007;33:136-43.
  31. Hwang CM, Sant S, Masaeli M, Kachouie NN, Zamanian B, Lee SH, et al. Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering. Biofabrication. 2010;2:035003 (12 pp). https://doi.org/10.1088/1758-5082/2/3/035003
  32. Kim GW, Choi YJ, Kim MS, Park Y, Lee KB, Kim IS, et al. Synthesis and evaluation of hyaluronic acid-poly(ethylene oxide) hydrogel via Michael-type addition reaction. Curr Appl Phy. 2007;7(1):28-32. https://doi.org/10.1016/j.cap.2005.06.009

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