Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
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Inhibition of odontogenic differentiation of human dental pulp cells by dental resin monomers

  • Kwon, Ji Hyun (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Park, Hee Chul (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Zhu, Tingting (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Yang, Hyeong-Cheol (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University)
  • Received : 2015.02.02
  • Accepted : 2015.03.26
  • Published : 2015.06.30
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Abstract

Background: Dental resin monomers that are leached from the resin matrix due to incomplete polymerization can affect the viability and various functions of oral tissues and cells. In this study, the effects of triethylene glycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA) on odontogenic differentiation of human dental pulp cells (HDPCs) were examined. To mimic clinical situations, dental pulp cells were treated with resin monomers for 24 h prior to the analysis of alkaline phosphatase (ALP) activity and mRNA expression of genes related to pulp cell differentiation. To elucidate the underlying signaling pathways, regulation of mitogen-activated protein (MAP) kinases by resin monomers was also investigated. Results: The ALP activity of HDPCs was reduced by TEGDMA and HEMA at noncytotoxic concentrations. The mRNA expression of dentin sialophosphoprotein (DSPP), osteocalcin (OCN), and osteopontin (OPN) was also downregulated by resin monomers. However, DSPP expression was not affected by hydrogen peroxide ($H_2O_2$). Among the MAP kinases examined, ERK activation (ERK phosphorylation) was not affected by either resin monomers or $H_2O_2$, whereas JNK was phosphorylated by TEGDMA and HEMA. Phospho-p38 was upregulated by HEMA, while TEGDMA and $H_2O_2$ suppressed p38 phosphorylation. Conclusions: Exposure to TEGDMA and HEMA for a limited period suppresses differentiation of HDPCs via different signaling pathways.

Keywords

Acknowledgement

Supported by : Ministry of Health & Welfare

References

  1. Cramer NB, Stansbury JW, Bowman CN. Recent advances and developments in composite dental restorative materials. J Dent Res. 2011;90:402-16. https://doi.org/10.1177/0022034510381263
  2. Peutzfeldt A. Resin composites in dentistry: the monomer systems. Eur J Oral Sci. 1997;105:97-116. https://doi.org/10.1111/j.1600-0722.1997.tb00188.x
  3. Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A, et al. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials. 2007;28:3757-85. https://doi.org/10.1016/j.biomaterials.2007.04.044
  4. Geurtsen W, Spahl W, Leyhausen G. Residual monomer/additive release and variability in cytotoxicity of light-curing glass-ionomer cements and compomers. J Dent Res. 1998;77:2012-9. https://doi.org/10.1177/00220345980770121001
  5. Michelsen VB, Lygre H, Skalevik R, Tveit AB, Solheim E. Identification of organic eluates from four polymer-based dental filling materials. Eur J Oral Sci. 2003;111:263-71. https://doi.org/10.1034/j.1600-0722.2003.00033.x
  6. Janke V, von Neuhoff N, Schlegelberger B, Leyhausen G, Geurtsen W. TEGDMA causes apoptosis in primary human gingival fibroblasts. J Dent Res. 2003;82:814-8. https://doi.org/10.1177/154405910308201010
  7. Spagnuolo G, D'Anto V, Cosentino C, Schmalz G, Schweikl H, Rengo S. Effect of N-acetyl-L-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts. Biomaterials. 2006;27:1803-9. https://doi.org/10.1016/j.biomaterials.2005.10.022
  8. Bakopoulou A, Leyhausen G, Volk J, Koidis P, Geurtsen W. Effects of resinous monomers on the odontogenic differentiation and mineralization potential of highly proliferative and clonogenic cultured apical papilla stem cells. Dent Mater. 2012;28:327-39. https://doi.org/10.1016/j.dental.2012.01.002
  9. Kim NR, Lim B-S, Park HC, Son KM, Yang H-C. Effects of N-acetylcysteine on TEGDMA- and HEMA-induced suppression of osteogenic differentiation of human osteosarcoma MG63 cells. J Biomed Mater Res B Appl Biomater. 2011;98B:300-7. https://doi.org/10.1002/jbm.b.31852
  10. Schweikl H, Hiller KA, Eckhardt A, Bolay C, Spaqnuolo G, Stempfl T, et al. Differential gene expression involved in oxidative stress response caused by triethylene glycol dimethacrylate. Biomaterials. 2008;29:1377-87. https://doi.org/10.1016/j.biomaterials.2007.11.049
  11. Lewis TS, Shapiro PS, Ahn NG. Signal transduction through MAP kinase cascades. Adv Cancer Res. 1998;74:49-139. https://doi.org/10.1016/S0065-230X(08)60765-4
  12. Zhang W, Liu HT. MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res. 2002;12:9-18. https://doi.org/10.1038/sj.cr.7290105
  13. Tibbles LA, Woodgett JR. The stress-activated protein kinase pathways. Cell Mol Life Sci. 1999;55:1230-54. https://doi.org/10.1007/s000180050369
  14. MacFarlane M, Cohen GM, Dickens M. JNK(c-Jun N-terminal kinase) and p38 activation in receptor-mediated and chemically induced apoptosis of T-cells; differential requirements for caspase activation. Biochem J. 2000;348(Pt1):93-101. https://doi.org/10.1042/bj3480093
  15. Obata T, Brown GE, Yaffe MB. MAP kinase pathways activated by stress: the p38 MAPK pathway. Crit Care Med. 2000;28:N67-77. https://doi.org/10.1097/00003246-200004001-00008
  16. Silva GA, Lanza LD, Lopes-Junior N, Moreira A, Alves JB. Direct pulp capping with a dentin bonding system in human teeth: a clinical and histological evaluation. Oper Dent. 2006;31:297-307. https://doi.org/10.2341/05-65
  17. Horsted-Bindslev P, Vilkinis V, Sidlauskas A. Direct capping of human pulps with a dentin bonding system or with calcium hydroxide cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96:591-600. https://doi.org/10.1016/S1079-2104(03)00155-0
  18. Lee DH, Kim NR, Lim B-S, Lee YK, Hwang KK, Yang H-C. Effects of root canal sealers on lipopolysaccharide-induced expression of cyclooxygenase-2 mRNA in murine macrophage cells. J Endod. 2007;33:1329-33. https://doi.org/10.1016/j.joen.2007.07.042
  19. Kim NR, Lee DH, Ahn SJ, Lee IS, Yang H-C. The differentiation-inducing effect of conditioned media obtained from dental pulp cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:54-9.
  20. Geurtsen W, Spahl W, Muller K, Leyhausen G. Aqueous extracts from dentin adhesives contain cytotoxic chemicals. J Biomed Mat Res. 1999;48:772-7. https://doi.org/10.1002/(SICI)1097-4636(1999)48:6<772::AID-JBM2>3.0.CO;2-X
  21. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res. 1995;74:1602-6. https://doi.org/10.1177/00220345950740091601
  22. Yoshii E. Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J Biomed Mat Res. 1997;37:517-24. https://doi.org/10.1002/(SICI)1097-4636(19971215)37:4<517::AID-JBM10>3.0.CO;2-5
  23. Geurten W. Substances released from dental resin composites and glassionomer cements. Eur J Oral Sci. 1998;106:687-95. https://doi.org/10.1046/j.0909-8836.1998.eos10602ii04.x
  24. Wang H, Joseph JA. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med. 1999;27:612-6. https://doi.org/10.1016/S0891-5849(99)00107-0

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