Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Comparison of transport media for the isolation and detection of Brachyspira hyodysenteriae

돈적리 균의 분리, 검출을 위한 수송배지의 비교

  • Cho, Se-Ji (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Kim, Jong Wan (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Young (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Oh, Sang-Ik (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Jeong, So Jeong (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Jung, Ji-A (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Cho, Ara (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Lee, Myoung-Heon (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Cho, Ho-Seong (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Byun, Jae-Won (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
  • Received : 2014.08.29
  • Accepted : 2015.01.19
  • Published : 2015.03.31
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Abstract

Brachyspira (B.) hyodysenteriae is a causative agent of swine dysentery that is responsible for death and economic losses in the pig industry. It is imperative that clinical samples be delivered fresh for accurate diagnosis. The viability and DNA detection of B. hyodysenteriae using lab-made (phosphate buffered saline and modified tryptic soy broth) or commercial transport media (C, D, and E) were compared by culturing and real-time PCR at $4^{\circ}C$ or room temperature (RT), respectively. B. hyodysenteriae grown in D (Anaerobe Systems, USA) and E (Starplex Scientific, Canada) media was viable for 4 days at $4^{\circ}C$ and RT. However, B. hyodysenteriae in A, B, and C (culture swab; BD Biosciences, USA) media were not recovered after 2 days at RT. Ct values for real-time PCR at $4^{\circ}C$ and RT ranged from $27.2{\pm}2.1$ (C) to $29.6{\pm}0.5$ (B), and $28.0{\pm}0.9$ (E) to $30.2{\pm}1.5$ (B), respectively. Considering the field conditions, it is important that transport media is used for specimen isolation and PCR to obtain an accurate diagnosis of swine dysentery.

Keywords

References

  1. Achacha M, Messier S. Comparison of six different culture media for isolation of Treponema hyodysenteriae. J Clin Microbiol 1992, 30, 249-251.
  2. de Barcellos DESN, Mathiesen M, Duhamel G. Survival of pathogenic intestinal spirochetes kept in pure cultures and in pig feces held at four different temperatures. Acta Sci Vet 2002, 30, 151-157.
  3. Boye M, Baloda SB, Leser TD, Moller K. Survival of Brachyspira hyodysenteriae and B. pilosicoli in terrestrial microcosms. Vet Microbiol 2001, 81, 33-40. https://doi.org/10.1016/S0378-1135(01)00328-5
  4. Chia SP, Taylor DJ. Factors affecting the survival of Treponema hyodysenteriae in dysenteric pig faeces. Vet Rec 1978, 103, 68-70. https://doi.org/10.1136/vr.103.4.68
  5. Cocolin L, Rantsiou K. Quantitative polymerase chain reaction in food microbiology. In: Filion M (ed.). Quantitative Real-time PCR in Applied Microbiology. pp. 149-160. Caister Academic Press, Norfolk, 2012.
  6. Duhamel GE, Bernard RJ, Mathiesen MR, Eskridge KM. Comparison of six commercially available transport media for maintenance of Serpulina (Treponema) hyodysenteriae. J Vet Diagn Invest 1992, 4, 285-292. https://doi.org/10.1177/104063879200400310
  7. Gibb AP, Wong S. Inhibition of PCR by agar from bacteriological transport media. J Clin Microbiol 1998, 36, 275-276.
  8. Hampson DJ. Brachyspiral colitis. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW (ed.). Diseases of swine. 10th ed. pp. 680-689. Wiley-Blackwell, Chichester, 2012.
  9. Hampson DJ, Atyeo RF, Combs BG. Swine dysentery. In: Hampson DJ, Stanton TB (eds.). Intestinal Spirochaetes in Domestic Animals and Humans. pp. 680-689. CAB international, Wallingford, 1997.
  10. Hindiyeh M, Acevedo V, Carroll KC. Comparison of three transport systems (Starplex StarSwab II, the new Copan Vi- Pak Amies Agar Gel collection and transport swabs, and BBL Port-A-Cul) for maintenance of anaerobic and fastidious aerobic organisms. J Clin Microbiol 2001, 39, 377-380. https://doi.org/10.1128/JCM.39.1.377-380.2001
  11. Karni M, Zidon D, Polak P, Zalevsky Z, Shefi O. Thermal degradation of DNA. DNA Cell Biol 2013, 32, 298-301. https://doi.org/10.1089/dna.2013.2056
  12. Kinyon JM, Harris DL, Glock RD. Enteropathogenicity of various isolates of Treponema hyodysenteriae. Infect Immun 1977, 15, 638-646.
  13. Patterson AH, Rubin JE, Fernando C, Costa MO, Harding JCS, Hill JE. Fecal shedding of Brachyspira spp. on a farrow-to-finish swine farm with a clinical history of "Brachyspira hampsonii"-associated colitis. BMC Vet Res 2013, 9, 137. https://doi.org/10.1186/1746-6148-9-137
  14. Rubin JE, Costa MO, Hill JE, Kittrell HE, Fernando C, Huang Y, O'Connor B, Harding JCS. Reproduction of mucohaemorrhagic diarrhea and colitis indistinguishable from swine dysentery following experimental inoculation with "Brachyspira hampsonii" strain 30446. PLoS One 2013, 8, e57146. https://doi.org/10.1371/journal.pone.0057146
  15. Stanton TB, Rosey EL, Kennedy MJ, Jensen NS, Bosworth BT. Isolation, oxygen sensitivity, and virulence of NADH oxidase mutants of the anaerobic spirochete Brachyspira (Serpulina) hyodysenteriae, etiologic agent of swine dysentery. Appl Environ Microbiol 1999, 65, 5028-5034.
  16. Suh DK, Do YJ, Ha JS, Lee KH, Cho YJ, Song DJ, Lee CS, Bae YC, Choi WP, Lee KW, Song JC. Prevalence of Brachyspira hyodesenteriae on selected swine farms in Gyeongbuk province by PCR. Korean J Vet Serv 2001, 24, 331-334.
  17. Suh DK, Do YJ, Ha JS, Lee KH, Song DJ, Lee CS, Bae YC, Jung SC, Choi WP, Lee KW, Song JC. Rapid detection of Brachyspira hyodysenteriae in swine intestinal specimens by PCR. Korean J Vet Serv 2001, 24, 335-341.