Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea

국내에서 발생하는 Apple scar skin viroid 분리주에 대한 계통분석

  • Cho, Kang Hee (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, In-Soo (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kil, Eui-Joon (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Park, Seo Jun (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Se Hee (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, In-Myung (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Dae-Hyun (National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 조강희 (농촌진흥청 국립원예특작과학원) ;
  • 김인수 (농촌진흥청 국립원예특작과학원) ;
  • 길의준 (성균관대학교 유전공학과) ;
  • 박서준 (농촌진흥청 국립원예특작과학원) ;
  • 김세희 (농촌진흥청 국립원예특작과학원) ;
  • 최인명 (농촌진흥청 국립원예특작과학원) ;
  • 김대현 (농촌진흥청 국립원예특작과학원)
  • Received : 2015.10.06
  • Accepted : 2015.11.12
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

To identify genome sequences of Apple scar skin viroid (ASSVd) isolates in Korea, the field survey was performed from 'Hongro' apple orchards located in eight sites in South Korea (Bongwha, Cheongsong, Dangjin, Gimchoen, Muju, Mungyeong, Suwon, and Yeongwol). ASSVd was detected by RT-PCR and PCR fragments were cloned into cloning vector. Full-length viral genomes of eight ASSVd isolates were sequenced and compared with 21 isolates reported previously from Korea, India, China, Japan and Greece. Eight isolates in this study showed 92.2-99.7% nucleotide sequence identities with those reported previously. Phylogenetic analysis showed that seven isolates reported in this study belong to the same group distinct from other groups.

국내에서 재배되는 사과 '홍로'에 발생하는 Apple scar skin viroid병의 유전자 계통분석을 하기 위하여 바이로이드 증상이 나타나는 8개 지역(봉화, 청송, 당진, 김천, 무주, 문경, 수원, 영월)의 농가에서 수집한 시료를 RT-PCR 방법을 이용하여 바이로이드 검정을 실시하였다. 검출된 바이로이드의 클로닝과 유전자 염기서열 분석을 통해 8종의 분리주들을 확인하였고 한국, 인도, 중국, 일본 및 그리스에서 보고된 21종의 분리주와 염기서열을 비교하였다. 8개의 분리주들의 핵산 서열은 기존에 보고된 분리주들과 92.2-99.7%의 상동성을 나타냈다. 계통분석을 통해 본 연구에서 보고한 7종의 분리주들은 기존의 것들과 독립된 그룹에 속하는 것으로 나타났다.

Keywords

References

  1. Cambell, A. and Sparks, T. R. 1976. Experiments with dapple apple virus. Acta Hortic. 67: 261-264.
  2. Diener, T. O. 1987. The viroids.Plenum Press, New York and London.
  3. Di Serio, D. F., Aparicio, F., Alioto, D., Ragozzino, A. and Flores, R. 1996. Identification and molecular properties of a 306-nucleotide viroid associated with apple dimple fruit disease. J. Gen. Virol. 77: 2833-2837. https://doi.org/10.1099/0022-1317-77-11-2833
  4. Gambino, G., Perrone, I. and Gribaudo, I. 2008. A rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants. Phytochem. Anal. 19: 520-525. https://doi.org/10.1002/pca.1078
  5. Hashimoto, J. and Koganezawa, H. 1987. Nucleotide sequence and secondary structure of apple scar skin viroid. Nucl. Acids Res. 15: 7045-7052. https://doi.org/10.1093/nar/15.17.7045
  6. Kwon, M. J., Hwang, S. L., Lee, S. J., Lee, D. H. and Lee, J. Y. 2002. Detection and distribution of the apple scar skin viroid-Korean strain (ASSVd-K) from apples cultivated in Korea. J. Plant Pathol. 18: 342-344. https://doi.org/10.5423/PPJ.2002.18.6.342
  7. Lee, G., Kim, J. H., Kim, H. R., Shin, I. S., Cho, K. H., Kim, S. H., Shin, J. and Kim, D. H. 2013. Production system of virus-free apple plants using heat treatment and shoot tip culture. Plant Dis. 19: 288-293. https://doi.org/10.5423/RPD.2013.19.4.288
  8. Lee, J. H., Park, J. K., Lee, D. H., Uhm, J. Y., Ghim, S. Y. and Lee, J. Y. 2001.Occurrence of apple scar skin viroid-Korean strain (ASSVd-K) in apples cultivated in Korea. Plant Pathol. J. 17: 300-304.
  9. Millikan, D. F. and Martin, W. R. 1956. An unusual fruit dimple symptom in apple. Plant Dis. Rep. 40: 229-230.
  10. Ministry of Agriculture, Food and Rural Affairs (MAFRA). 2015. MAFRA yearbook. Agriculture statistics, 2015.
  11. Ohtsuka, Y. 1938. On Manshu-sabika-byo of apple, graft transmission and symptom variation in cultivars. J. Jpn. Soc. Hortic. Sic. 9: 282-286. https://doi.org/10.2503/jjshs.9.282
  12. Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30: 2725-2729. https://doi.org/10.1093/molbev/mst197
  13. Yamaguchi, A. and Yanase, H. 1976. Possible relationship between the causal agent of dapple apple and scar skin. Acta Hort. 67: 249-254.
  14. Zhu, S. F., Hadidi, A., Hammond, R. W., Yang, X. and Hansen, A. J. 1995. Nucleotide sequence and secondary structure of pome fruit viroids from dapple apple diseased apples, pear rusty skin diseased pears, and apple scar skin symptomless pears. Acta Hort. 386: 554-559.