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http://dx.doi.org/10.5423/RPD.2015.21.4.346

Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea  

Cho, Kang Hee (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, In-Soo (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kil, Eui-Joon (Department of Genetic Engineering, Sungkyunkwan University)
Park, Seo Jun (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Se Hee (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Choi, In-Myung (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Dae-Hyun (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Publication Information
Research in Plant Disease / v.21, no.4, 2015 , pp. 346-350 More about this Journal
Abstract
To identify genome sequences of Apple scar skin viroid (ASSVd) isolates in Korea, the field survey was performed from 'Hongro' apple orchards located in eight sites in South Korea (Bongwha, Cheongsong, Dangjin, Gimchoen, Muju, Mungyeong, Suwon, and Yeongwol). ASSVd was detected by RT-PCR and PCR fragments were cloned into cloning vector. Full-length viral genomes of eight ASSVd isolates were sequenced and compared with 21 isolates reported previously from Korea, India, China, Japan and Greece. Eight isolates in this study showed 92.2-99.7% nucleotide sequence identities with those reported previously. Phylogenetic analysis showed that seven isolates reported in this study belong to the same group distinct from other groups.
Keywords
Apple scar skin viroid; Phylogenetic analysis; Sequencing;
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Times Cited By KSCI : 2  (Citation Analysis)
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