The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants

  • Lee, Siwon (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Kim, Jin-Ho (Department of Chemistry, College of Natural Science, Dankook University) ;
  • Choi, Ji-Young (Department of Chemistry, College of Natural Science, Dankook University) ;
  • Jang, Won-Cheoul (Department of Chemistry, College of Natural Science, Dankook University)
  • Received : 2015.06.12
  • Accepted : 2015.08.12
  • Published : 2015.12.01
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Abstract

We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.

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References

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