Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Study of the enzymatic action of the chymopapain using pluronic based nano-carrier system on the cadaveric nucleus pulposus tissue

플루로닉(pluronic) 기반의 나노운반체(nano-carrier)에 충진된 카이모파파인(chymopapain)의 척추 추간판 조직내 작용성에 관한 연구

  • Choi, Won Il (Life Science Research Institute, Daewoong Pharm) ;
  • Tae, Gi Yoong (School of Materials Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Hong, Young Ki (Department of Sports Medicine, Cheongju University)
  • 최원일 (대웅제약 생명과학연구소) ;
  • 태기융 (광주과학기술원 신소재공학부) ;
  • 홍영기 (청주대학교 스포츠의학과)
  • Received : 2014.10.29
  • Accepted : 2015.01.08
  • Published : 2015.01.31
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Abstract

The objective of this study is to determine if when chymopapain is loaded onto a nano-carrier, an injection of it reduces the spreading range of the drug within the discs. The materials for the experiment, which were conducted for three weeks, included fifteen intervertebral discs taken from two cadavers, which were divided according to the types of injected chymopapain solutions as follows: ordinary chymopapain group and nano-carrier system group. The nano-carrier system group was again divided into two subgroups according to the types of pluronics, the basic material for the nano-carriers: Pluronic F 127(DA-PF 127) in the nano-carrier group and Pluronic F 68(DA-PF 68) in the nano-carrier group. The results showed that the action of chymopapain using a pluronic-based nano-carrier system was localized around the center of the injection site instead of broad spreading, compared to that of the ordinary chymopapain group (p<0.01). This characteristic suggests a possible application to effective agents for minimally invasive spinal treatment through which disc lesions were removed selectively.

이 연구의 목적은 척추 추간판 융해제인 카이모파파인을 나노캐리어 시스템에 충진시켜 주입시, 추간판내 약제의 확산범위가 감소하는지 평가하기 위한 것이다. 총 3주간 시행된 실험의 재료로는 두 개의 카데바에서 채취된 열다섯 개의 척추 추간판이 쓰였고, 이들은 주입되는 카이모파파인의 종류에 따라 일반 카이모파파인 그룹과 나노캐리어 시스템 그룹으로 나뉘었다. 나노캐리어 시스템 그룹은 다시 그 기반재료가 되는 플루로닉의 종류에 따라 플루로닉 F 127(DA-PF 127) 나노캐리어 그룹과 플루로닉 F 68(DA-PF 68) 나노캐리어 그룹으로 나뉘었다. 실험결과 나노캐리어 시스템을 사용한 그룹은 일반 카이모파파인 그룹에 비해, 척추 추간판 내부로 주입되었을 때, 주입지점에서 멀리 확산되지 않고 국소적으로 작용하는 특성을 보였다(p<0.01). 이러한 특성은 향후 추간판 내 병소만을 선택적으로 제거하는 최소침습적 척추시술의 기반약제로 응용될 가능성을 제시해준다.

Keywords

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