한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제28권1호
  • /
  • Pages.41-52
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  • 2015
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
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매괴화(玫瑰花) 에탄올추출물이 α-MSH로 유도된 과색소 형성 억제와 작용기전 연구

Inhibitory Effect of the Ethanol Extract of Rosae rugosae Flos on the Hyperpigmentation and its Action Mechanism Induced by α-MSH

  • 이진호 (원광대학교 한의학전문대학원 BK21-plus팀) ;
  • 인명희 (원광대학교 한의학전문대학원 BK21-plus팀) ;
  • 강석훈 (원광대학교 한약연구소 약학대학 한약학과) ;
  • 문연자 (원광대학교 한의학전문대학원 BK21-plus팀) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실) ;
  • 임규상 (원광대학교 한국전통의학연구소)
  • Lee, Jin-Ho (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • In, Myung-Hee (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kang, Suk-Hoon (Dept. of Oriental Pharmacy, College of pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Mun, Yeun-Ja (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Dept. of Anatomy, College of Oriental Medicine, Wonkwang University) ;
  • Lim, Kyu-Sang (Research Center of Traditional Korean Medicine, Wonkwang University)
  • 투고 : 2015.01.09
  • 심사 : 2015.02.05
  • 발행 : 2015.02.25
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초록

Objective : This study investigated the inhibitory mechanism of the hypopigmentating effects on ethanol extract of Rosae rugosae Flos (ERR) that has not yet been examined. Methods : We analyzed the anti-melanogenic effects of ethanol extracts from Rosae rugosae Flos by tyrosinase activity, melanin contents. We also examined protein expression levels of tyrosinase, TRP-1, TRP-2, MITF and ERK by western blot analysis in melanoma cells. Results : In this investigation, ERR effectively reduced ${\alpha}$-MSH-stimulated melanin synthesis by suppressing expression of tyrosinase and tyrosinase-related protein-1 (TRP-1). On the other hand, the expression of tyrosinase-related protein-2 (TRP-2) were not affected by treatment with ERR. ERR inhibited the expression of microphthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expression regulating melanogenesis. The upstream signaling pathway including cAMP response element-binding protein (CREB) and MAPKs were also inhibited by ERR. Pretreatment with PD98059, ERK inhibitor, attenuated the inhibitory effect of ERR on ${\alpha}$-MSH-induced tyrosinase activity. Conclusions : Our study suggested that the anti-melanogenic activity of ERR is correlated with the suppression of tyrosinase gene through CREB/MITF/ERK pathway.

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참고문헌

  1. Kang TC, Go JS, Kwon OY, Gi YG, Kim KY. Human histology. Seoul. Ko Moon Sa. 2012;7:263.
  2. Tsukamoto K, Jackson IJ, Urabe K, Montague PM, Hearing VJ. A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. The Journal of the European Molecular Biology Organization. 1992;11(2): 519-26.
  3. Costin GE, Hearing VJ. Human skin pigmentation. melanocytes modulate skin color in response to stree. The Journal of the Federation of American Societies for Experimental Biology. 2007;21:976-94. https://doi.org/10.1096/fj.06-6649rev
  4. Kim, DS, Hwang ES, Lee JE, Kim SY, Kwon SB, Park KC. Sphingosine-1-phosphate decreases melanin synthesis via sustained ERK activation and subsequent MITF degradation. Journal of Cell Science. 2003;116:1699-706. https://doi.org/10.1242/jcs.00366
  5. Hong WS. Jing Xiao Huang Di Ne Jjing Su Wen. Seoul. Oriental Medicine Institute Press. 1981:179.
  6. Lu ying. Yi Xue Gang Mu. Seoul. Bu Bin Publishers Company. 2010:681-2, 509, 510.
  7. Hua S. Zhong Hua Yi Shu Ji Cheng. Nan Jing Ben Yi. Beijing. Traditional Chinese Medicine Ancient Publishing Company 1999;1(3):12.
  8. Zhou ZH. Dan Xi Yi Ji. Beijing. People's Health publisher. 1995:1(2):511, 513.
  9. Zhao Ji. Sheng Ji Zong Lu. Beijing. People's Health publisher. 1987:1763.
  10. Zhou MS. Yi Xue Bao Jian. Seoul. Haeng Rim Seo Won. 1975:186-7.
  11. Heo J. Dong Ui Bo Gam. Seoul. Han Mi Medical Publishing Co. 2001:268-9, 271.
  12. Qi K. Wai Ke Da Cheng. Munkwang library Limited. 1968:218.
  13. Chen SG. Wai Ke Zheng Zong. Beijing. Traditional Chinese medicine Ancient publishing company.1999;1:248.
  14. Gu SC. Yang Yi Da Quan. Beijing. People's Health publisher. 1987:1:479-82.
  15. Gynaecology Oriental Medicine Compilation Committee. Korean Medicine Gynecology & Obstetrics. Seoul. Jeong Dam. 2002:2(2):431.
  16. Ju YS. Ungok Herbology. Jeonju. Woosuk Press. 2013;2(2):846-7.
  17. Kim DS, No ST, Lee JC, Lim KS, Shin MR, Woo WH, Mun YJ. Inhibitory effect of the ethanol extract of fagopyrum escuentum on melanin synthesis. The Journal of Tradition Korean Medicne. 2006;15(1):70-6
  18. Lee JS, Kim JA, Cho SH, Son AR, Jang TS, So MS, Chung SR, Lee SH. Tyrosinase Inhibitors isolated from the Roots of Glycyrrhiza glabra L. Korean Journal of Pharmacognosy. 2003;34(1):33-9.
  19. Lim NY, Kwon KJ, Kim YS, Baek SG, Lim JR, Mun YJ, Woo WH. Inhibitory Effect of Methanolic Extract from Adenophorae Radix on Melanogenesis. Korean Journal of Oriental Physiology & Pathology. 2004;18(3):747-53.
  20. Yu HS, Mun YJ, Woo WH, Song JH. Anti-Melanogenic Effects of Ethanol Extracts from Rosa rugosa Thunb. Department of Beauty Desing Graduate School of Wonkwang University. 2014;20(1):39-41.
  21. Hwang JG, Yun JK, kim SK, Lee SH, Han KH. Mass Production of Chaff-vinegar and Its Effect of Anti-Aging and Whitening. Journal of Microbiology and Biotechnology. 2012;40 (3):208-14.
  22. Kim DS, Sung BG, Lee JC, Lee BK, Woo WH, Lim KS. Inhibitory Effect of Belamcandae Rhizoma on the Melanogenesis in MSH-stimulated B16F10 cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2011;24(1):25-35.
  23. Provance DW, Jr, Wei M, Ipe V, Mercer JA. Cultured melanocytes from dilute mutant mice exhibit dendritic morphology and altered melanosome distribution. Proceedings of the National Academy Sciences of the United States of America. 1996;93:14554-8. https://doi.org/10.1073/pnas.93.25.14554
  24. Bertolotto C, Busca' R, Abbe P, Bille K, Aberdam E, Ortonne JP, Ballotti R. Different cis-acting element are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP: pivotal role of M boxes (GTCATGTGCT) and of microphthalmia. Molecular and Cellular Biology. 1998;18: 694-702. https://doi.org/10.1128/MCB.18.2.694
  25. Hunt G, Todd C, Cresswell JE, Thody AJ. Alpha-melanocyte stimulating hormone and its analogue Nle4DPhe7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes. Journal of Cell Science. 1994;107 (Pt 1):205-11.
  26. Rodrigez-Lo'pez JN, Tudela J, Var'on R, Garci'a-Carmona F, Garcia-Ca'novas F. Analysis of a kinetic model for melanin biosynthesis pathway. The Journal of Biological Chemistry. 1992;267:3801-10.
  27. Jang JY, Kim HN, Kim YR, Kim BY, Choi YH. Studies of Inhibitory Mechanism on Melanogenesis by Partially Purified Asiasari radix in α-MSH Stimulated B16F10 Melanoma Cells. Journal of Life Science. 2010;20(11);1617-24. https://doi.org/10.5352/JLS.2010.20.11.1617

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