The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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An Experimental Study on the Effect of Angelica acutiloba Ethanol Extract on Hyaluronic Acid Synthesis

일당귀 에탄올 추출물의 Hyaluronic Acid 합성 효과에 대한 실험적 연구

  • Kang, Min-Seo (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Semyung University) ;
  • Ha, Hun-Yong (Dept. of Natural Medicine Resources, Semyung University) ;
  • Kim, Hee-Taek (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Semyung University)
  • 강민서 (세명대학교 한의과대학 안이비인후피부과학 교실) ;
  • 하헌용 (세명대학교 자연약재과학과) ;
  • 김희택 (세명대학교 한의과대학 안이비인후피부과학 교실)
  • Received : 2015.01.15
  • Accepted : 2015.02.09
  • Published : 2015.02.25
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Abstract

Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and anti-aging. The purpose of this study is to examine the effects of Angelica acutiloba ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There was no cytotoxicity in $50{\mu}g/ml$ concentration Angelica acutiloba extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration Angelica acutiloba extract. Hyaluronic acid production was higher in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration Angelica acutiloba extract than control group. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin.

Keywords

References

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