Parasites, Hosts and Diseases

  • 제53권5호
  • /
  • Pages.597-603
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  • 2015
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Activation of MAPK Is Required for ROS Generation and Exocytosis in HMC-1 Cells Induced by Trichomonas vaginalis-Derived Secretory Products

  • Narantsogt, Giimaa (Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Min, Arim (Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Nam, Young Hee (Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Lee, Young Ah (Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Kim, Kyeong Ah (Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Agvaandaram, Gurbadam (Department of Basic Science, School of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences) ;
  • Dorjsuren, Temuulen (Department of Basic Science, School of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences) ;
  • El-Benna, Jamel (DR-CNRS Centre de Recherche Biomedical, INSERM U773, Universite Paris & Denis Diderot) ;
  • Shin, Myeong Heon (Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine)
  • 투고 : 2015.05.31
  • 심사 : 2015.08.14
  • 발행 : 2015.10.31
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초록

Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen-activated protein kinase (MAPK), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. In this study, we investigated whether MAPK is involved in ROS generation and exocytotic degranulation in HMC-1 cells induced by T. vaginalis-derived secretory products (TvSP). We found that TvSP induces the activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and $p47^{phox}$ in HMC-1 cells. Stimulation with TvSP also induced up-regulation of CD63, a marker for exocytosis, along the surfaces of human mast cells. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation. Finally, our results suggest that TvSP induces intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling.

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