Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene

  • Wang, Ju-Hua (College of Animal Science and Technology, Anhui Agriculture University) ;
  • Xue, Xiu-Heng (College of Tea & Food Technology, Anhui Agriculture University) ;
  • Zhou, Jie (College of Animal Science and Technology, Anhui Agriculture University) ;
  • Fan, Cai-Yun (College of Animal Science and Technology, Anhui Agriculture University) ;
  • Xie, Qian-Qian (College of Animal Science and Technology, Anhui Agriculture University) ;
  • Wang, Pan (College of Animal Science and Technology, Anhui Agriculture University)
  • Received : 2014.12.24
  • Accepted : 2015.04.10
  • Published : 2015.06.30
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Abstract

Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of $Ca^{2+}$, $Mg^{2+}$, $K^+$, and $HCO_3{^-}$ in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.

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References

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