Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb

  • Oh, Chang Seok (Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine) ;
  • Seo, Min (Department of Parasitology and Research Center for Mummy, Dankook University) ;
  • Hong, Jong Ha (Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Oh, Seung Whan (Hangang Institute of Cultural Heritage) ;
  • Park, Jun Bum (Sangmyung University) ;
  • Shin, Dong Hoon (Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine)
  • Received : 2014.12.11
  • Accepted : 2015.03.03
  • Published : 2015.04.30
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Abstract

Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.

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References

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