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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antioxidant Activity of the Halophyte Ligustrum japonicum

염생식물 광나무(Ligustrum japonicum)의 항산화 활성

  • Baek, Seung Oh (Division of Marine Bioscience, Korea Maritime & Ocean University) ;
  • Kim, Hojun (Division of Marine Bioscience, Korea Maritime & Ocean University) ;
  • Jeong, Heejeong (Ocean Science & Technology School, Korea Maritime & Ocean University) ;
  • Ju, Eunsin (Division of Marine Bioscience, Korea Maritime & Ocean University) ;
  • Kong, Chang-Suk (Department of Food and Nutrition, College of Medical & Life Sciences, Silla University) ;
  • Seo, Youngwan (Division of Marine Bioscience, Korea Maritime & Ocean University)
  • 백승오 (한국해양대학교 해양생명과학부) ;
  • 김호준 (한국해양대학교 해양생명과학부) ;
  • 정희정 (한국해양대학교 해양과학기술전문대학원) ;
  • 주은신 (한국해양대학교 해양생명과학부) ;
  • 공창숙 (신라대학교 식품영양학과) ;
  • 서영완 (한국해양대학교 해양생명과학부)
  • Received : 2015.10.17
  • Accepted : 2015.12.07
  • Published : 2015.12.27
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Abstract

Dried samples of Ligustrum japonicum were extracted twice: with methylene chloride and with methanol (MeOH), respectively. The combined crude extracts were successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. Antioxidant activities of crude extract and its solvent fractions were evaluated by measuring authentic $ONOO^-$, and $ONOO^-$ generated from 3-morpholinsydnonimine (SIN-1) as well as degree of occurrence of intracellular ROS in HT 1080 cells, and genomic DNA oxidation. The 85% aq.MeOH and n-BuOH fractions exhibited the good antioxidant activity. Further purification of the 85% aq.MeOH fracition led to the isolation of Oleanolic acid (1), Maslinic acid (2), and Ursolic acid (3). All compounds showed the significant antioxidant effects in all assay systems.

Keywords

References

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