Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Potential Association between Insulin-like Growth Factor-1 Receptor Activity and Surf Clam Spisula sachalinensis Larvae Survival

  • Choi, Youn Hee (Institute of Fisheries Sciences, Pukyong National University) ;
  • Nam, Taek Jeong (Institute of Fisheries Sciences, Pukyong National University)
  • Received : 2015.09.21
  • Accepted : 2015.11.08
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the relationship between viability and IGF-1 receptor (IGF-1R) activity in D-shaped and umbo larvae of the surf clam Spisula sachalinensis after treatment with vitrification solution (VS) or freezing. In a toxicity assay, VS1, containing 5 M dimethyl sulfoxide (DMSO), was very harmful to D-shaped and umbo larvae. However, VS2, containing 5 M ethylene glycol (EG), was not harmful to either larval stage. Although VS2 had a promising toxicity test outcome, none of the larvae survived vitrification. After immersion into VSs and freezing, IGF-1R ${\beta}$-subunits were detected in all larvae; however, tyrosine phosphorylation of intracellular ${\beta}$-subunits was detected only in the control and live groups. These results suggest that activation of IGF-1R may influence surf clam larvae viability.

Keywords

References

  1. Adams TE, Epa VC, Garrett TPJ and Ward CW. 2000. Structure and function of the type 1 insulin-like growth factor receptor. Cell Mol Life Sci 57, 1050-1093. https://doi.org/10.1007/PL00000744
  2. Chao NH, Lin TT, Chen YJ, Hsu HW and Liao IC. 1997. Cryopreservation of late embryos and early larvae in the oyster and hard clam. Aquaculture 155, 31-44. https://doi.org/10.1016/S0044-8486(97)00107-5
  3. Chen SL and Tian YS. 2005. Cryopreservation of flounder (Paralichthys olivaceus) embryos by vitrification. Theriogenology 63, 1207-1219., https://doi.org/10.1016/j.theriogenology.2004.06.007
  4. Choi YH, Nam TJ and Kuwano K. 2013. Cryopreservation of gametophytic thalli of Porphyra yezoensis (Rhodophyceae) by one-step fast cooling. J Appl Phycol 25, 531-535. https://doi.org/10.1007/s10811-012-9887-0
  5. Codina M, de la serrana DG, Sanchez-Gurmaches J, Montserrat N, Chistyakova O, Navarro I and Gutierrez J. 2008. Metabolic and mitogenic effects of IGF-II in rainbow trout (Oncorhynchus mykiss) myocytes in culture and the role of IGF-II in the PI3K/Akt and MAPK signalling pathways. Gen Comp Endocrinol 157, 116-124. https://doi.org/10.1016/j.ygcen.2008.04.009
  6. Cuevas-Uribe R, Leibo SP, Daly J and Tiersch TR. 2011. Production of channel catfish with sperm cryopreserved by rapid non-equilibrium cooling. Cryobiology 63, 186-197. https://doi.org/10.1016/j.cryobiol.2011.06.004
  7. Czech MP. 1989. Signal transmission by the insulin-like growth factors. Cell 59, 235-238. https://doi.org/10.1016/0092-8674(89)90281-X
  8. Duan C. 1997. The insulin-like growth factor system and its biological actions in fish. Ame Zool 37, 491-503. https://doi.org/10.1093/icb/37.6.491
  9. Duan C and Xu Q. 2005. Roles of insuli-like growth factor (IGF) binding proteins in regulating IGF actions. Gen Comp Endocrinol 142, 44-52. https://doi.org/10.1016/j.ygcen.2004.12.022
  10. Gwo JC 1995. Cryopreservation of oyster (Crassostrea gigas) embryos. Theriogenology 43, 1163-1174. https://doi.org/10.1016/0093-691X(95)00088-P
  11. Hagedorn M and Kleinhans FW. 2011. Problems and prospects in cryopreservation of fish embryos, in: T.R. Tiersch, C.C. Green (Eds.), Cryopreservation in Aquatic Species, second ed., World Aquaculture Society, Baton Rouge, Louisiana, USA, 483-502.
  12. Paredes E, Bellas J and Adams S. 2013. Comparative cryopreservation study of trochophore larvae from two species of bivalves: Pacific oyster (Crassostrea gigas) and blue mussel (Mytilus galloprovincialis). Cryobiology 67, 274-279. https://doi.org/10.1016/j.cryobiol.2013.08.007
  13. Robles V, Cabrita E, de Paz P, Cunado, Anel L and Herraez MP. 2004. Effect of a vitrification protocol on the lactate dehydrogenase and glucose-6-phosphate dehydrogenase activities and the hatching rates of zebrafish (Danio rerio) and turbot (Scophthalmus maximus) embryos. Theriogenology 61, 1367-1379. https://doi.org/10.1016/j.theriogenology.2003.08.010
  14. Robles V, Cabrita E, Acker JP and Herraez P. 2008. Embryo cryopreservation: what we know until now. CRC Press, Taylor & Francis Group, London.
  15. Tian YS, Chen SL, Yan AS, Ji XS and Yu GC. 2003. Studies on vitrification method of sea perch (Lateolabrax japonicus) embryos. Acta Zool Sinica 49, 843-850.
  16. Zhang T and Rawson DM. 1996. Feasibility studies on vitrification of intact zebrafish (Brachydanio rerio). Cryobiology 33, 1-13. https://doi.org/10.1006/cryo.1996.0001