Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Root proteome analysis of Chinese cabbage in response to Plasmodipohora brassicae Woron

배추 무사마귀병 마커 탐색을 위한 배추 뿌리 단백질체 분석

  • Jeung, Jae Yun (Department of Crop Science and Biotechnology, Dankook University) ;
  • Lim, Yong Pyo (Department of Horticulture, Chungnam National University) ;
  • Hwang, Cheol Ho (Department of Crop Science and Biotechnology, Dankook University)
  • 정재윤 (단국대학교 식량생명공학과) ;
  • 임용표 (충남대학교 원예학과) ;
  • 황철호 (단국대학교 식량생명공학과)
  • Received : 2015.11.03
  • Accepted : 2015.12.17
  • Published : 2015.12.31
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Abstract

Clubroot disease is one of the most wide-spread and devastating diseases in the cultivation of Chinese cabbage. To develop a protein marker for resistance to clubroot disease in Chinese cabbage, a comparative proteome analysis was performed between a sensitive line, 94SK, and a resistant line, CR Shinki DH. Three proteins of two fold or higher accumulation that are specific to each line were found 3 days after innoculation of the Plasmodiphora brassicae. They are glutamine synthetase, malate dehydrogenase/oxidoreductase and fructose-bisphosphate aldolase in the 94SK and actin, phosphoglycerate kinase, and Cu/Zn superoxide dismutase in the CR Shinki line. From the comparison of the synthesized proteins in the 94SK and the CR Shinki, CR Shinki was found to produce more ATP-binding protein for the ABC transporter while 94SK showed a higher level of pathogenesis-related protein 1 production. All of these proteomic variations may lead to the development of molecular markers to accelerate the breeding process.

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References

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