DOI QR코드

DOI QR Code

Asymbiotic germination and seedling growth of Calanthe striata f. sieboldii Decne. ex Regel

금새우난초(Calanthe striata f. sieboldii Decne. ex Regel)종자의 비공생 발아 및 신초증식

  • Received : 2015.05.22
  • Accepted : 2015.09.15
  • Published : 2015.09.30

Abstract

Calanthe striata f. sieboldii Decne. ex Regel is a terrestrial orchid with beautiful flowers arranged in racemose inflorescences. This species is threatened due to over-collection and loss of suitable habitats. Asymbiotic germination is useful in the conservation efforts to re-establish plants in the wild, and for commercial propagation. In this study, we investigate the effects of sodium hypochlorite (NaOCl), three types of culture media (Phytomax Orchid Maintenance - POM, Seed Germination Maintenance - SGM, and Murashige and Skoog 1962 - MS), and plant growth regulators on embryo swelling, protocorm formation, and embryo diameter of C. striata f. sieboldii. Treatment with 1% NaOCl for 30 min greatly enhanced embryo swelling (28.3%), embryo diameter ($205.8{\mu}m$), and embryo protocorm formation (54.8%) compared to seeds without NaOCl treatment (embryo swelling 8.5%, embryo diameter $14.6{\mu}m$, and protocorm formation 13.4%) on POM medium. Protocorm formation on POM medium supplemented with 1.0 mg/L N6-benzyladenine (BA) (95.6%) was better than the control (54.5%). Additionally, the effects of activated charcoal (AC) and sucrose on seedling growth in in vitro culture were examined. The protocorm converted into healthy plants with well-developed shoot primordia on the POM medium with AC and sucrose. The most suitable conditions for seedling growth after 10 weeks of culture were the POM medium with AC or sucrose. These results show effective asymbiotic seed germination and in vitro seedling growth of C. striata f. sieboldii.

본 연구는 자생 난초과식물인 금새우난초의 기내 비대배 유도, 발아 및 배직경에 미치는 몇 가지 영향(NaOCl, 배지, 식물생장조절제)을 조사하기 위해 수행되었다. 1% NaOCl을 30분간 처리한 후 POM배지에 배양할 경우, 비대배 유도는 28.3%, 발아율은 54.8%, 배직경은 $205.8{\mu}m$로 가장 높게 조사되었다. NaOCl 무처리 종자를 POM배지에 배양할 경우, 비대배 유도는 8.5%, 발아율은 13.4%, 배직경은 $14.6{\mu}m$로 조사되었다. 또한 식물생장조절물질의 처리가 발아에 미치는 영향을 조사한 결과, 1.0 mg/L의 BA 처리구(95.6%)의 발아율은 대조구(54.5%)에 비해 1.75배 높게 조사되었다. AC와 sucrose의 처리 농도가 기내 식물체 생장에 미치는 영향을 조사한 결과, 엽장은 10 g/L의 sucrose 처리구에서 6.8 cm로 가장 길었고, 대조구는 3.3 cm로 가장 짧았다. 근장은 50 g/L의 sucrose 처리구에서 5.8 cm로 가장 길었고, 대조구는 1.7 cm로 가장 짧았다. 생중량과 건중량은 AC와 sucrose 처리구가 대조구에 비해 2~5배 이상 높은 결과를 보였다. 결과적으로 본 연구는 금새우난초의 기내 발아 및 증식에 미치는 몇 가지 요인에 관해 구체적인 결과를 제시하였고, 이러한 결과는 향후 자생 난초과식물의 증식 및 보존전략 개발에 중요한 기초자료로 제공 될 것이다.

Keywords

References

  1. Bae KH, Kim CH, Sun BY, Choi YE (2010) Structural changes of seed coats and stimulation of in vitro germination of fully mature seeds of Cypripedium macranthos Swartz by NaOCl pre-treatment. Prog Ornament Plant 10:107-113
  2. Bae KH, Choi YE (2013) Factors affecting fruit baring in natural habitate and in vitro culture of zygotic embryos of Cypripedium japonicum. Prog Ornament Plant 11:146-152
  3. Bae KH, Kwon HK, Choi YE (2009) In vitro germination and plantlet conversion from the culture of fully mature seeds of Cypripedium guttatum Swartz. Prog Ornament Plant 9:160-165
  4. Bae KH, Ko MS, Choi SA, Lee HB, Kim NY, Song JM, Song G (2012) In vitro germination of Gastrodia verrucosa Blume and Hetaeria sikokiana Tuyama treated by NaOCl. J Plant Biotechnol 39:163-168 https://doi.org/10.5010/JPB.2012.39.3.163
  5. Bae KH, Oh KH, Kim SY (2014) Sodium hypochlorite treatment and light-emitting diode (LED) irradiation effect on in vitro germination of Oreorchis patens (Lindl.) Lindl. J Plant Biotechnol 41:44-49 https://doi.org/10.5010/JPB.2014.41.1.44
  6. Chung MY, Chung JD, Jee SO (1998) Effect of culture media on asymbiotic seed germination and those seedling growth of Calanthe discolor and Habenaria radiata. Kor J Plant Tiss Cult 25:189-194
  7. Dumas E, Monteuuis O (1995) In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants influence of activated charcoal. Plant Cell Tiss Org Cult 40:231-235 https://doi.org/10.1007/BF00048128
  8. Eymar E, Alegre J, Toribio M, Lopez-vela D (2000) Effect of activated charcoal and 6-benzyladenine on in vitro nitrogen uptake by Lagerstroemia indica. Plant Cell Tiss Org Cult 63:57-65 https://doi.org/10.1023/A:1006471519609
  9. Gabriela F, Carlos T, Carlos O, Yves D, Jorge MS (2005) Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets. In Vitro Cell Devel Biol 41:69-76 https://doi.org/10.1079/IVP2004597
  10. Gale S, Drinkell C (2007) Calanthe arisanensis Orchidaceae. Curtis's Bot. Mag. 24:206-210 https://doi.org/10.1111/j.1467-8748.2007.00595.x
  11. Godo T, Komori M, Nakaoki E, Yukawa T, Miyoshi K (2010) Germination of mature seeds of Calanthe tricarinata Lindl., an endangered terrestrial orchid, by asymbiotic culture in vitro. In Vitro Cell Dev Biol Plant 46:323-332 https://doi.org/10.1007/s11627-009-9271-1
  12. Hyun MR, Choi JY, Suh JN, So IS, Lee JS (1999a) Studies on distributions and morphological characteristics of Calanthe discolor, C. sieboldii, and C. bicolor native to Cheju Province. Kor J Hort Sci Technol 17:498-500
  13. Hyun MR, Choi JY, Suh JN, So IS, Lee JS (1999b) Isozyme and randomly amplified polymorphic DNA (RAPD) analysis for genetic relationship among Calanthe discolor, C. sieboldii, and C. bicolor native to Cheju Island. Kor J Hort Sci Technol 17:141-143
  14. Kano K (1972) The orchids. Seibundo-shinkosha, Tokyo
  15. Kim BC, Kim MH, Oh MY (1990) A taxonomic study on Calanthe in Cheju island - A comparative study on isozyme by electrophoresis. Kor J Plant Tax 20:53-64 https://doi.org/10.11110/kjpt.1990.20.1.053
  16. Kim CH, Chung JD (1997) Asymbiotic germination of Korean native Calanthe species. Agric Res Bull Kyungpook Natl Univ 15:47-52
  17. Kim KS, Kim JS, Park JH (2008) Aseptic germination of F1 hybrid seed by inter-species pollination of Calanthe discolor Lindl, and C. discolor for. Sieboldii (Decne.) Ohwi. Kor J Plant Res 21:341-345
  18. Kim YS, Kim SH (1989) A taxonomic study on Calanthe in Korea. Kor J Plant Tax 19:273-287 https://doi.org/10.11110/kjpt.1989.19.4.273
  19. Lee, EJ, Koh JC (2002) Improvement of seed germination in native Iris sanguinea donn ex horn. Kor J Hort Sci Technol. 20:345-351
  20. Lee GJ, Kim KS, Kim JS, Park JH, Kim JB, Kim DS, Kang SY (2008) A wide array of phenotypic components and their contributions to variations in Calanthe orchid land races. Hort Environ Biotechnol 49:418-426
  21. Lee JS, Bae JH, Yoo SO (1993) Useful Plant Resources in Mt. Moak Sited Jeonlabuk-do, Korea. J Kor Flower Res Soc 2:55-72
  22. Lee JS, Choi BH (2006) Distribution and red data of wild orchids in the Korean Peninsula. Kor J Plant Taxon 36:335-360 https://doi.org/10.11110/kjpt.2006.36.4.335
  23. Lee K (1980) The wild orchids of Korea. Jungsung publisher. Seoul. Korea. p. 193
  24. Lee NS (2011) Illustrated flora of Korean Orchids. Ewha Womans University publisher. Seoul. Korea. p. 208
  25. Lee YN (2006) Flora of Korea. Gyohaksa, Seoul
  26. Magdalena K, Anna B (2005) Morphogenesis of Lilium martagon L. explants in callus culture. Acta Biol Craco Series Bot 47:65-73
  27. Mathews VH, Rao PS (1980) In vitro multiplications of Vanda hybrids through tissue culture technique. Plant Sci Lett 17:383-389 https://doi.org/10.1016/0304-4211(80)90171-6
  28. Miyoshi K, Mii M (1988) Ultrasonic treatment for enhancing seed germination of terrestrial orchid, Calanthe discolor, in asymbiotic culture. Scientia Horticulturae 35:127-130 https://doi.org/10.1016/0304-4238(88)90044-1
  29. Miyoshi K, Mii M (1995a) Phytohormone pre-treatment for the enhancement of seed germination and protocorm formation by the terrestrial orchid, Calanthe discolor (Orchidaceae), in asymbiotic culture. Scientia Horticulture 63:263-267 https://doi.org/10.1016/0304-4238(95)00813-9
  30. Miyoshi K, Mii M (1995b) Enhancement of seed germination and protocorm formation in Calanthe discolor (Orchidaceae) by NaOCl and poly phenol absorbent treatments. Plant Tiss Cult Lett 12:267-272 https://doi.org/10.5511/plantbiotechnology1984.12.267
  31. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  32. Pan MJ, van Staden J (1998) The use of charcoal in in vitro culture-a review. Plant Growth Regul 26:155-163 https://doi.org/10.1023/A:1006119015972
  33. Park SY, Murthy NH, Paek KY (2000) In vitro seed germination of Calanthe sieboldii, an endangered orchid species. J Plant Biology 43:158-161 https://doi.org/10.1007/BF03030493
  34. Takayama S, Misawa M (1979) Differentiation in Lilium bulbscales grown in vitro: Effect of various cultural conditions. Physi Planta 46:184-190 https://doi.org/10.1111/j.1399-3054.1979.tb06555.x