Journal of Plant Biotechnology

  • 제42권3호
  • /
  • Pages.235-238
  • /
  • 2015
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  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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셍온(Paraserianthes falcataria (L.) Nielsen)의 기내 부정줄기 유도 및 식물체 재분화

In vitro introduction adventitious shoots and plant regeneration of sengon (Paraserianthes falcataria (L.) Nielsen)

  • 김지아 (국립산림과학원 산림생명공학과) ;
  • 문흥규 (국립산림과학원 산림생명공학과) ;
  • 김용욱 (국립산림과학원 산림생명공학과) ;
  • 배은경 (국립산림과학원 임목육종과)
  • Kim, Ji Ah (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Moon, Heung Kyu (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Kim, Yong Wook (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Bae, Eun Kyung (Division of Tree Improvement, Korea Forest Research Institute)
  • 투고 : 2015.09.02
  • 심사 : 2015.09.23
  • 발행 : 2015.09.30
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초록

최대의 부정아유도수는 MS배지에 0.2 mg/L BA가 첨가된 배지에서 유도되었다. 엽병근처 자엽 절편과 자엽 끝 절편을 MS배지에 0.2 mg/L BA가 첨가된 배지에 배양하였다. 부정아 유도 빈도는 절편형태에 따라 차이를 보였다. 엽병근처 자엽 절편은 자엽 끝 절편 보다 부정아 유도율이 높았다. 호르몬 무처리 1/2MS배지에서 줄기의 신장과 뿌리가 유도되었다. 이 결과는 클론번식을 가능하게 할 효과적인 방법을 제공한다.

Adventitious buds were obtained from isolated cotyledons cultured on MS medium with various concentrations of 6-benzylamino purine (BA) and thidiazuron (TDZ). The highest numbers of adventitious buds were obtained on MS medium supplemented with 0.2 mg/L BA. Experimental culturing with half the petiole portion and half with the terminal segments were grown on MS medium contained with 0.2 mg/L BA. Frequency of the adventitious bud induction was variable accordingly to the type of cultured explants. Explants with the half petiole showed the highest adventitious bud induction rate (80%) compared to explants of half with terminal segment (20%). An elongated shoot from the buds and growth of advent roots were both possible on the 1/2 MS medium without a plant growth regulator. These results offer an effective way in which clonal propagation can be accomplished.

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참고문헌

  1. Bon MC, Bonal D, Goh DK, Monteuuis O (1998) Influence of different macronutrient solutions and growth regulators on micropropagation of juvenile shape Acacia mangium and shape Paraserianthes falcataria explants. Plant Cell Tiss Org Cult 53:171-177 https://doi.org/10.1023/A:1006068505548
  2. Ghosh N, Smith DW, Das AB, Chatterjee A (2010) Rapid in vitro regeneration and clonal propagation of the fastest growing leguminous tree Albizia falcataria (L.) Fosberg using leaflet explant. Plant Tissue Cult and Biotech 20:63-72
  3. Ichihashi Y, Kawade K, Usami T, Horiguchi G, Takahashi T, Tsukaya H (2011) Key proliferative activity in the junction between the leaf blade and leaf petiole of Arabidopsis. Plant Physiol 157:1151-1162 https://doi.org/10.1104/pp.111.185066
  4. Kumar RS, Mallick R (1993) Regeneration and multiplication of shoot in Albizia falcataria. Plant Cell Tissue Organ Cult 32:259-261 https://doi.org/10.1007/BF00029851
  5. Kumar S, Sarkar AK, Kunhikannan C (1998) Regeneration of plants from leaflet explants of tissue culture raised safed siris (Albizia procera). Plant Cell Tissue Organ Cult 54:137-143 https://doi.org/10.1023/A:1006179611034
  6. Lloyd G and B McCown (1981) Commercially feasible micropropagation of mountain lavrel, kalmia latifolia, by use of shoot-tip culture. Comb Proc Intl Plant Prop Soc 30:421-427
  7. Murashige T and Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  8. Sasmitamihardja D, Hadisutanto Johannes S. and Widiyanto Sri N (2010) In Vitro Regeneration of Paraserianthes falcataria (L.) Nielsen. Proc. of the II International Symposium on Biotechnology of Tropical and Subtropical Species Taiwan. Taipei. pp167-170
  9. Sinha RK, Majumdar K, Sinha S (2000) In vitro differentiation and plant regeneration of Albizia chinesis (Osb.) Merr. In Vitro Cell Dev Biol Plant 36:370-373 https://doi.org/10.1007/s11627-000-0066-7
  10. Varis E (2011) Stand growth and management scenarios for Paraserianthes falcataria smallholder plantations in Indonesia. University of Helsinki. M. Sc. Degree. pp3-17