한국섬유공학회지 (Textile Science and Engineering)

  • 제52권6호
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  • Pages.373-378
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  • 2015
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  • 1225-1089(pISSN)
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  • 2288-6419(eISSN)
한국섬유공학회 (The Korean Fiber Society)
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형광 공액화 고분자전해질과 DNA 앱타머의 복합체를 사용한 단백질 센서

A Protein Sensory System Comprising a Fluorescent Conjugated Polyelectrolyte and DNA Aptamer

  • 손지혜 (충남대학교 유기소재.섬유시스템공학과) ;
  • 김충호 (충남대학교 유기소재.섬유시스템공학과) ;
  • 이택승 (충남대학교 유기소재.섬유시스템공학과)
  • Son, Jihye (Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University) ;
  • Kim, Choongho (Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University) ;
  • Lee, Taek Seung (Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University)
  • 투고 : 2015.12.02
  • 심사 : 2015.12.14
  • 발행 : 2015.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

A water-soluble anionic conjugated polyelectrolyte was successfully synthesized via the Suzuki cross-coupling reaction in the presence of a palladium catalyst. Using this fluorescent polyelectrolyte, a new concept for a rapid, label-free lysozyme-sensing method is proposed via possible naked-eye detection of the emission color change. Intermolecular exciton migration in the conjugated polyelectrolyte-based complex was adopted to enhance the selectivity and sensitivity for lysozyme sensing by the formation and dissociation of the polymer-lysozyme assay complex in the absence and presence of the anti-lysozyme aptamer, respectively. The polymer-lysozyme complex showed red emission because of cooperative aggregation of the conjugated polyelectrolyte and lysozyme. Upon exposure to the aptamer, the complex dissociated into individual molecules, resulting in a transparent blue-emitting solution. This occurred because lysozyme is released from the complex by the aptamer via the more favorable binding between the two molecules (lysozyme and aptamer).

키워드

참고문헌

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