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Antioxidant Activities of n-Butanol and Ethyl Acetate Extracts from Yam (Dioscorea batatas DECNE.)

  • Duan, Yishan (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Gyeong-Hwuii (Department of Biomaterials Science and Engineering, Yonsei University) ;
  • Seong, Jong-Hwan (Department of Food Science and Technology, Pusan National University) ;
  • Chung, Hun-Sik (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • Received : 2015.08.23
  • Accepted : 2015.09.01
  • Published : 2015.12.30

Abstract

This study aimed at evaluating the antioxidant activity of raw yam (Dioscorea batatas $D_{ECNE.}$) extracted by n-butanol and ethyl acetate. The antioxidant activities, including ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, and ferric reducing antioxidant power as well as total phenol contents were studied in vitro. Results showed ethyl acetate extract ($111.88{\pm}0.66mg\;CAE/g$) possessed higher total phenol content than n-butanol extract ($78.68{\pm}0.58mg\;CAE/g$). Ethyl acetate extract exhibited stronger antioxidant activity compared with n-butanol extract apart from reducing power assay.

Keywords

References

  1. T. Bahorun, A. Luximon-Ramma, A. Crozier, O. I. Aruoma, Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables, J. Sci. Food Agric., 84, 1553 (2004). https://doi.org/10.1002/jsfa.1820
  2. M. S. Blois, Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199 (1958). https://doi.org/10.1038/1811199a0
  3. I. F. F. Benzie, J. J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay, Anal. Biochem., 239, 70 (1996). https://doi.org/10.1006/abio.1996.0292
  4. L. Barros, M. J. Ferreira, B. Queiros, Isabel C. F. R. Ferreira, P. Baptista, Total phenols, ascorbic acid, ${\beta}$-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities, Food Chem., 103, 413 (2007). https://doi.org/10.1016/j.foodchem.2006.07.038
  5. M. R. Bhandari, J. Kawabata, Organic acid, phenolic content and antioxidant activity of wild yam (Dioscorea spp.) tubers of Nepal, Food Chem., 88, 163 (2004). https://doi.org/10.1016/j.foodchem.2003.12.027
  6. E. C. Cadenas, K. J. A. Davies, Mitochondrial free radical generation, oxidative stress, and aging. Free Radi. Biolo. Med., 29, 222 (2000). https://doi.org/10.1016/S0891-5849(00)00317-8
  7. Chou, S. T., Chiang, B. H., Chung, Y. C., Chen, P. C., Hsu, C. K. Effects of storage temperatures on the antioxidative activity and composition of yam, Food Chem., 98, 618 (2006). https://doi.org/10.1016/j.foodchem.2005.06.039
  8. E. M. Choi, S. J. Koo, J. K. Hwang, Immune cell stimulating activity of mucopolysaccharide isolated from yam (Dioscorea batatas), J. Ethnopharmacology, 91, 1 (2004). https://doi.org/10.1016/j.jep.2003.11.006
  9. Y. S. Duan, M. A. Kim, J. H. Seong, H. S. Chung, H. S. Kim, Antioxidative activities of various solvent extracts from haw (Crataegus pinnatifida Bunge), Korean J. Food Preserv., 21, 246 (2014). https://doi.org/10.11002/kjfp.2014.21.2.246
  10. I. Gulcin, Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid), Toxicology, 217, 213 (2006). https://doi.org/10.1016/j.tox.2005.09.011
  11. G. R. Zhao, Z. J. Xiang, T. X. Ye, Y. J. Yuan, Z. X. Guo, Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng, Food Chem., 99, 767 (2006). https://doi.org/10.1016/j.foodchem.2005.09.002
  12. D. Harrison, K. K. Griendling, U. Landmesser, B. Hornig, H. Drexler, Role of oxidative stress in atherosclerosis, American J. Cardiology, 91, 7 (2003). https://doi.org/10.1016/S0002-9149(02)03144-2
  13. C. S. Hariprakash, B. Nambisan, Carbohydrate metabolism during dormancy and sprouting in yam (Dioscorea) tubers: changes in carbohydrate constituents in yam (Dioscorea) tubers during dormancy and sprouting, J. Agricul. Food Chem., 44, 3066 (1996). https://doi.org/10.1021/jf950784d
  14. C. L. Hsu, W. Chen, Y. M. Weng, C. Y. Tseng, Chemical composition, physical properties, and antioxidant activities of yam flours as affected by different drying methods, Food Chem., 83, 85 (2003). https://doi.org/10.1016/S0308-8146(03)00053-0
  15. S. Y. Kim, H. J. Jwa, Y. Yanagawa, T. S. Park, Extract from Dioscorea batatas ameliorates insulin resistance in mice fed a high-fat diet, J. Med. Food, 15, 527 (2012). https://doi.org/10.1089/jmf.2011.2008
  16. M. P. Kahkonen, A. I. Hopia, H. J. Vuorela, J. P. Rauha, K. Pihlaja, T. S. Kujala, M. Heinonen, Antioxidant activity of plant extracts containing phenolic compounds, J. Agric. Food Chem., 47, 3954 (1999). https://doi.org/10.1021/jf990146l
  17. M. Laguerre, J. Lecomte, P. Villeneuve, Evaluation of the ability of antioxidants to counteract lipid oxidation: Existing methods, new trends and challenges, Progress in Lipid Research, 46, 244 (2007). https://doi.org/10.1016/j.plipres.2007.05.002
  18. J. T. Lin, D. J. Yang, Determination of steroidal saponins in different organs of yam (Dioscorea pseudojaponica Yamamoto), Food Chem., 108, 1068 (2008). https://doi.org/10.1016/j.foodchem.2007.11.041
  19. B. B. Li, B. Smith, M. M. Hossain, Extraction of phenolics from citrus peels I. solvent extraction method, Separa. Puri. Technol., 48, 182 (2006). https://doi.org/10.1016/j.seppur.2005.07.005
  20. L. S. Lai, S. T. Chou, W. W. Chao, Studies on the antioxidative activities of hsian-tsao (Mesona procumbens Hemsl) leaf gum, J Agric. Food Chem., 49, 963 (2001). https://doi.org/10.1021/jf001146k
  21. L. Migliore, F. Coppede, Genetic and environmental factors in cancer and neurodegenerative diseases, Mutation Res., 512, 135 (2002). https://doi.org/10.1016/S1383-5742(02)00046-7
  22. P. S. Oh, K. T. Lim, Plant glycoprotein modulates the expression of interleukin-1${\beta}$ via inhibition of MAP kinase in HMC-1 cells, Biosci. Biotechnol. Biochem., 72, 2133 (2008). https://doi.org/10.1271/bbb.80204
  23. P. S. Oh, K. T. Lim, Antioxidant activity of Dioscorea batatas Decne glycoprotein, European Food Res. Technol., 226, 507 (2008). https://doi.org/10.1007/s00217-007-0563-6
  24. V. E. C. Ooi, F. Liu, Immunomodulation and anti-Cancer Activity of polysaccharide-protein complexes, Current Med. Chem., 7, 715 (2000). https://doi.org/10.2174/0929867003374705
  25. R. Prior, G. H. Cao, Antioxidant phytochemicals in fruits and vegetables: diet and health implications, Hort Sci., 35, 588 (2000).
  26. R. J. Robbins, Phenolic acids in foods: an overview of analytical methodology, J. Agricul. Food Chem., 51, 2866 (2003). https://doi.org/10.1021/jf026182t
  27. V. Roginsky, E. A. Lissi, Review of methods to determine chain-breaking antioxidant activity in food, Food Chem., 92, 235 (2005). https://doi.org/10.1016/j.foodchem.2004.08.004
  28. M. Valko, D. Leibfritz, J. Moncol, M. T. D. Cronin, M. Mazur, J. Telser, Free radicals and antioxidants in normal physiological functions and human disease, Inte. J. Biochem. Cell Biol., 39, 44 (2007). https://doi.org/10.1016/j.biocel.2006.07.001
  29. Y. S. Velioglu, G. Mazza, L. Gao, B. D. Oomah, Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products, J. Agric. Food Chem., 46, 4113 (1998). https://doi.org/10.1021/jf9801973
  30. A. Wojdylo, J. Oszmianski, R. Czemerys, Antioxidant activity and phenolic compounds in 32 selected herbs, Food Chem., 105, 940 (2007). https://doi.org/10.1016/j.foodchem.2007.04.038

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