The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effect of Lime Sulfur on Changes of Fungal Diversity in Pear Fallen Leaves

석회유황합제가 배나무 낙엽의 진균 다양성 변화에 미치는 영향

  • Min, Kwang-Hyun (Department of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Song, Jang Hoon (Pear Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cho, Baik Ho (Department of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Yang, Kwang-Yeol (Department of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University)
  • 민광현 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 송장훈 (농촌진흥청 국립원예특작과학원 배연구소) ;
  • 조백호 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 양광열 (전남대학교 농업생명과학대학 식물생명공학부)
  • Received : 2015.11.23
  • Accepted : 2015.12.14
  • Published : 2015.12.31
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Abstract

This study was conducted to examine changes in the fungal community on fallen leaves of pear by treatment with lime sulfur. Although the lime sulfur could reduce the primary inoculum of several pathogens on spring season, the effect of lime sulfur has not been well determined scientifically. Fallen leaves infected by pear diseases in pear orchards in Naju were collected and treated with lime sulfur or water as a control. To determine the fungal diversity from each treatment, rDNA internal transcribed spacer (ITS) regions were analyzed after extraction of fungal genomic DNA from lime sulfur-treated or water-treated fallen leaves, respectively. The most common fungal species were Ascomycota and Basidiomycota in both treated leaves. However, the population dynamics of several fungal species including Alternari sp., Cladosporium sp., and Phomopsis sp., which are known as pear pathogens for skin sooty dapple disease, were quite different from each treated leaves. These results indicated that lime sulfur treatment led to changes of fungal communities on pear fallen leaves and could be applicable as a dormant spray.

석회유황합제의 배나무 동계방제 수단으로써 효과를 과학적으로 증명하기 위해 배 과수원에서 1차 전염원의 역할을 하는 낙엽에 석회유황합제를 처리한 후 낙엽에 존재하는 진균의 다양성 변화를 확인하였다. 물이 처리된 대조구와 석회유황합제 처리구의 낙엽에서 진균 genomic DNA를 추출한 다음 rDNA internal transcribed spacer (ITS) 영역의 염기서열 분석을 통해 진균들을 동정한 결과, 대조구와 석회유황합제 처리구에서 자낭균문(Ascomycota)과 담자균문(Basidiomycota)에 속하는 다양한 종류의 진균들이 동정되었으나 동정되는 진균의 수와 비율은 서로 다르게 나타났다. 특히 배 과피얼룩병을 일으키는 Alternaria속과 Cladosporium속의 진균은 대조구에 비해 석회유황합제 처리구에서 동정되는 비율이 대폭 감소되었으며, Phomopsis속의 진균은 대조구에서는 동정되었으나 석회유황합제 처리구에서는 동정되지 않았다. 이러한 결과는 석회유황합제 처리가 배 낙엽에 존재하는 진균 다양성의 변화에 영향을 미쳤으며 동계방제로서 역할을 수행할 수 있음을 시사한다.

Keywords

References

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