The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Identification of Yeasts from Jeju Island Soils

제주도 토양에서 효모의 분리 및 동정

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Bae, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Han, Jae-Won (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ji-Yoon (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 배상민 (배재대학교 바이오.의생명공학과) ;
  • 한재원 (배재대학교 바이오.의생명공학과) ;
  • 김지윤 (배재대학교 바이오.의생명공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과) ;
  • 김하근 (배재대학교 바이오.의생명공학과)
  • Received : 2015.12.05
  • Accepted : 2015.12.10
  • Published : 2015.12.31
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Abstract

Significant differences in annual precipitates on Jeju island have been reported depending on the location. We collected soil samples from east and west areas of Jeju Island to identify yeasts by plating on yeast peptone dextrose plates and subsequent analysis for the polymerase chain reaction amplified D1/D2 region of 26S rDNA of colonies. As a result, 20 yeast strains of 12 species were isolated from 7 different sampling sites in east area and 13 yeast strains of 6 species from 5 different sampling sites in west area. Some differences in yeast flora were observed depending on the sample collection sites having different annual precipitates.

제주도 토양에 서식하는 야생효모들의 분포 특성을 조사하기 위해, 동부지역 7곳, 서부지역 5곳에서 토양을 채취하여 효모를 분리한 후, 콜로니 PCR에 의해 증폭된 26S rDNA D1/D2 지역의 염기서열을 결정하여 BLAST를 이용하여 효모를 동정하였다. 동부지역에서 채집한 토양에서 12종에 속하는 20균주를, 서부지역에서 6종에 속하는 13균주를 분리하였다. 분리되는 효모 종은 강수량 차이를 보이는 지역에 따라서 현저한 차이가 있는 것을 알 수 있었다.

Keywords

References

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